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Báo cáo khoa học: Characterization of mutations in crucial residues around the Qo binding site of the cytochrome bc1 complex from Paracoccus denitrificans pdf
Ngày tải lên :
23/03/2014, 10:20
... of the D278 mutant indicates the partial
contribution of this side chain to the signals of the
protonated acidic residues for the oxidized form in the
absence of the inhibitor. The shifts of the ... respectively, from the Q
o
binding site
(see Fig. 1), as measured from the chromone head-
group of the inhibitor [2]. Their involvement in a water
chain leading up to the heme b
L
edge, and their parti-
cipation ... observed for the Y302F and the Y297F
mutant enzymes. These shifts are rather small as com-
pared to previously published absorption coefficients
for these modes [23,25,28,29]. In the spectra of the
Y302F...
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Tài liệu Báo cáo khoa học: An investigation of the substrate specificity of the xyloglucanase Cel74A from Hypocrea jecorina pdf
Ngày tải lên :
19/02/2014, 05:20
... proteins,
including other GHs (clans E, F and J) [3] and hemo-
pexin [20]. The CD spectrum of the latter protein [21]
is very similar to the one observed here; therefore, the
unusual spectrum ... Avicel were obtained from Sigma-
Aldrich, and the cello-oligosaccharides were obtained from
Merck (Darmstadt, Germany). The oligosaccharides
derived from b-glucan were a gift from A Planas (Universi-
tat ... incubation with Cel74A, and the proposed cleavage pattern (C) with the
theoretical m ⁄ z value (z in superscript) of the most important fragments (the position of the Gal residues is arbitrary)...
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TYING ODYSSEUS TO THE MAST: EVIDENCE FROM A COMMITMENT SAVINGS PRODUCT IN THE PHILIPPINES* pot
Ngày tải lên :
06/03/2014, 10:20
... ODYSSEUS TO THE MAST
the product itself, rather than from simply being offered the
product. These effects support the point estimates discussed
above.
In order to calculate the Treatment on the Treated ... effects estimated in the pre-
vious specifications, and suggests that the impact of the commit-
ment product came from the product itself, and not from the
door-to-door marketing.
Further supporting ... period.
Comparing the treatment group to the control group, the
largest treatment effects—in both the six-month and one-year
periods—are for the very bottom of the distribution, the lowest
decile, and for the...
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Báo cáo Y học: The Fe-only nitrogenase and the Mo nitrogenase from Rhodobacter capsulatus A comparative study on the redox properties of the metal clusters present in the dinitrogenase components doc
Ngày tải lên :
18/03/2014, 01:20
... stems from t he P
3+
cluster, then the
g ẳ 2.27 feature derives from the FeFe c ofactor. On
the other hand, if the broad signal, d espite its unusual
properties, represents the P
1+
cluster, the ... absent.
The profile of the entire signal, without interference of the
rhombic s ignal, was obtained by subtracting the pH 6.4-
spectrum from the pH 8.4-spectrum (see the inset of Fig. 3).
The axial ... of these novel EPR signals to
either the cofactor or the P-cluster has proven elusive due to
the fact that both of these metal clusters present in the
Fe-only n itrogenase are d iamagnetic in the...
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Báo cáo khoa học: The effect of pH on the initial rate kinetics of the dimeric biliverdin-IXa reductase from the cyanobacterium Synechocystis PCC6803 pptx
Ngày tải lên :
23/03/2014, 05:22
... saturat-
ing) and the variation of initial rate with the concen-
tration of the second substrate is then used to estimate
k
cat
and the K
m
for the variable substrate. However,
the assumption that ... bound at the active site. The
Y102A mutant therefore accomodates a variant config-
uration of biliverdin to the wild-type enzyme but
retains the ability to catalyse the transfer of hydride
from both ... and
varying the concentration of NADPH from 5–100 lm
or NADH from 50–1000 lm. The ‘fixed’ concentra-
tions of biliverdin were also varied from 0.5–10 lm to
yield the plot for NADPH as the variable...
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Báo cáo Y học: Probing the rotor subunit interface of the ATP synthase from Ilyobacter tartaricus pptx
Ngày tải lên :
24/03/2014, 00:21
... changes in the HSQC spectrum of the cÂe complex by
mutating the cE204 residue to Gln. Inset in (B) indicates the
changes in the selected areas of the HSQC spectrum of the
cÂe complex imposed by the ... with the catalytic sites for ATP
synthesis, and the membrane-embedded F
o
domain
(bacterial subunits ab
2
c
10–15
), with the sites for the
translocation of the ions. In ATP synthesis mode, the
F
o
motor ... empty chip), allowing the elimination
of the latter by baseline correction (see below). As a result
of the location of the His tag on the very top of the c sub-
unit, the immobilized cÂe complexes...
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Tài liệu Báo cáo khoa học: Modulation of the enzymatic efficiency of ferredoxin-NADP(H) reductase by the amino acid volume around the catalytic site pptx
Ngày tải lên :
18/02/2014, 17:20
... in the protein stability of our mutants. There-
fore, these conclusions strengthen our hypothesis that
the catalytic efficiencies of the recombinant enzymes
used in our study were related to the ... transfer
from the two-electron substrate NADPH to Fd pro-
ceeds in an ordered pathway, in which the observed
K
m
value results from the sum of the K
m
values for the
successive interaction of the ... by DNA sequencing. The
design of the mutants was intended to preserve the
amino acid character and to modify only the relative
volume of their R groups.
The expression of the FNR mutants as soluble...
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Cambridge.University.Press.The.Crisis.of.Literature.in.the.1790s.Print.Culture.and.the.Public.Sphere.Nov.1999.pdf
Ngày tải lên :
21/09/2012, 11:00
... private rather than public terms, relating them
to the play of the imagination rather than the exercise of reason.
But these shifts cannot erase the important continuities that
existed between the ... elites it was intent on displacing on the one
hand and from the various popular and plebeian strata it aspired to rule
on the other.
19
The first of these shifts, from a focus on a cultural product
(literature) ... prefaced with a statement of the editor’s commitment to the
Enlightenment30
Literature has reconciled the whole thinking world respecting the great
principles of the system of the universe, and extirpated...
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Tài liệu Báo cáo khóa học: Mutations in the hydrophobic core and in the protein–RNA interface affect the packing and stability of icosahedral viruses doc
Ngày tải lên :
19/02/2014, 12:20
... Figure 5C shows that the spectra of the
sample subjected to compression and decompression are
similar to the non-treated sample, even in the case of the
M88V mutant. The elution of the samples after ... as the major virus structural protein,
forming the shell in which the RNA genome is
contained [11,12].
The tertiary structure and topology of the MS2 coat
protein is different from those of other ... interacts with residues on the adjacent b-strand (F) and
with amino acids in the alpha-helical regions as they pass
over the b-sheet. Thus, the M88V mutant substitution
affects the dimer interface,...
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Performance Audit of the Department of Corrections for the Legislative Service Bureau of the Oklahoma Legislature ppt
Ngày tải lên :
16/03/2014, 00:20
... prisoners, the nature of their offenses
or the lengths of their sentences in a timely manner. Since the demographics and criminal history
of these offenders are unknown, they cannot be factored into the ... in
most other jurisdictions, the growth of the corrections system has had a profound effect on the
state’s fiscal resources. The cost of the Oklahoma corrections system has tripled over the last ... open access to their facilities over the course of this
project. Without the assistance of the many line staff and managers who took time from there
assigned duties to explain their operations...
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The challenge of obesity in the WHO European Region and the strategies for response pptx
Ngày tải lên :
16/03/2014, 14:20
... children, but they should also consider adults, as their competence to
make healthy choices or their capacity to resist the marketing of unhealthy food may not fully protect them from
the damage ... things.
The dynamics of the food system, particularly in the expanding markets of eastern Europe, needs further ex-
ploration and the evolution of the food supply, particularly the price and availability ... increased over the past decade (Fig. 10).
4. The European Region has the highest alcohol consumption in the world, particularly among men (69).
Wine is generally preferred in southern Europe,...
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The Bible in its Making The most Wonderful Book in the World docx
Ngày tải lên :
16/03/2014, 15:20
... exactly the Egypt of ancient days is described in the Bible!
The dress the people wore, the food they ate, the way they spoke to their kings, the description of their
funerals, the very name of their ... through the walls of the Holy City. His ears rang with the cries of rage and despair which
broke from the Jews within, as one by one their defences fell, and the end drew near!
Then food failed in the ... BEHIND THE CLERK'S EAR]
So the questioners settled the matter in their own minds; but God had the answer to their questions all ready
for them.
He put into the hearts of some brave men the...
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Báo cáo khoa học: The starch-binding capacity of the noncatalytic SBD2 region and the interaction between the N- and C-terminal domains are involved in the modulation of the activity of starch synthase III fromArabidopsis thaliana pdf
Ngày tải lên :
22/03/2014, 21:20
... properties of the enzyme [19]. To extend
the information about the role of the noncatalytic
SBD regions and their effect on the C-terminal CD,
we further explored the amino acids of the N-terminal
region ... Whereas the production of ADPGlc via ADPGlc
PPase is the first committed step in starch biosynthe-
sis, SS catalyzes the elongation of a-1,4-glucans by
the transfer of the glucosyl moiety from the ... confirmed the results obtained in the
pull-down assays, showing that there is a physical
interaction between the N- and C-terminal domains of
SSIII in vitro.
Mapping of the CD-binding region in the
N-terminal...
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Báo cáo khoa học: Revisiting the 13C-label distribution of the non-oxidative branch of the pentose phosphate pathway based upon kinetic and genetic evidence doc
Ngày tải lên :
23/03/2014, 15:21
... Consequently, the
top two carbon atoms of s7p synthesized in the half-
reaction model can originate from either f6p or p5p,
while in the traditional model they can only originate
from p5p. In ... procedures
Metabolic network model
Apart from the variations in the stoichiometric model of
the PPP discussed in this work, the other parts of the stoi-
chiometric model used for fitting the fluxes of S. cerevisiae
were ... the
metabolites formed from these) can differ from the
13
C
labeling of the single C
2
and C
3
fragment pools gener-
ated by the half-reactions.
Genetic organization of the nonoxidative branch
of the PPP
In...
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Concentration in the Belgian brewing Industry and the Breakthrough of Lager in the interwar years pptx
Ngày tải lên :
31/03/2014, 22:20
... and acquisitions easier because it replaced most of the existing laws
concerning these activities. The law furthermore exempted taxes on the surplus capital by liquidation of
companies in case ... Market: The Brewing Case’ The Journal
of Industrial Economics, 13-2 (1965), p.129-153.; BROUWER, M., The European Beer industry;
Concentration and competition’ in: DE JONG, H.W.(ed.), The Structure ... and Concentration in the Brewing Industry’, The Journal of
Industrial Economics, 19-3 (1971), p. 206.; PELES, Y., ‘Economies of scale in advertising beer and
cigarettes’, The Journal of Business,...
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- khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản
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