comparative proteomic analysis of mesenchymal stem cells derived from human bone marrow umbilical cord and placenta implication in the migration

A multiplex comparative proteomic analysis of hypoxia influence in the presence and absence of p53 in HCT116 cells

Ngày tải lên : 16/09/2015, 15:43

... who wrote “Proteomics: the analysis of complete complements of proteins Proteomics includes not only the identification and quantification of proteins, but also the determination of their localization, ... hypoxia in the presence and absence of p53 4.6 A protein expression and interaction network of proteins, involved 80 in cellular growth, proliferation and cell cycle, in the absence of p53 under ... treatment and the presence of p53 An ontology analysis of these 54 proteins revealed that they were mainly involved in the regulation of cellular growth and proliferation Downstream validation analysis...

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báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx

Ngày tải lên : 18/06/2014, 15:20

... sorting), we obtained the expanded cells as a mixture of ECs and MCs derived from human ES cells (hESECs+MCs) The cell mixture was composed of almost the same number of ECs and MCs We resorted the ... characterization of transplanted cells derived from human ES cells We induced differentiation of human ES cells in an in vitro two-dimensional culture on OP9 stromal cell line and examined the expression of ... Characterization of the transplanted vascular cells derived from human ES cells (HES-3) Characterization of the transplanted vascular cells derived from human ES cells (HES-3) A, Flow cytometric analysis of...

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báo cáo hóa học:" Targeting of mesenchymal stem cells to ovarian tumors via an artificial receptor" docx

Ngày tải lên : 20/06/2014, 07:20

... and bioluminescence color images were superimposed using the Adobe Photoshop 7.0 software Statistics on bioluminescent signal intensity was obtained using WinView software according to the software ... measuring luc expression in tumors and other major organs of the peritoneal cavity (Fig and 6) At the indicated time points we also performed whole body bioluminescent imaging, imaging of individual ... validate this initial observation and to more accurately establish the timing of MSC homing, we investigated the kinetics of MSC tumor targeting The speed and pattern of cell vehicles homing to target...

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Báo cáo khoa học: "In vitro neuronal and osteogenic differentiation of mesenchymal stem cells from human umbilical cord blood" ppt

Ngày tải lên : 07/08/2014, 18:21

... llec gnimrofrep dna esu lacinilc rof SBF gnisu tuohtiw oviv xe sCSM dnapxe ot sdohtem dnif ot deen ew dna ,hcraeser ruo rof SBF desu ew ,yduts tneserp ni ,revewoH noitarefilorp sCSM rof noitidnoc ... 001-X notirT %4.0 neht dna ,nim rof enoteca %001 ,nim 01 rof lonahtem %001 dloc eci htiw detaert neht erew yehT erutarepmet moor ta nim 01 rof SBP ni edyhedlamrof %7.3 htiw dexif neht dna SBP ... noitcudni cinegoetso na desu ew ,sCSM eht morf noitaitnereffid cinegoetso ecudni oT laitnetop cinegoetso tibihxe sCSM doolb droC )A2 giF( nitnemiv dna 2HS snegitna detaler -myhcnesem rof ytivitisop...

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Báo cáo y học: "An in vitro study investigating the survival and phenotype of mesenchymal stem cells following injection into nucleus pulposus tissue" pot

Ngày tải lên : 09/08/2014, 01:22

... and 3,3-diaminobenzidine disclosure) for aggrecan and the staining intensity increased with time post injection (Figures and 5b) Aggrecan matrix staining within the vicinity of the injected cells ... performed the type X collagen and Alizarin red staining, and participated in interpretation of the data AJF participated in interpretation of the data and contributed to the preparation of the final ... following injection, and the intensity of the matrix staining increased with time in culture (Figures and 5b) Weak staining for type II collagen was observed within a small number of cells in monolayer...

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Báo cáo y học: "Immunomodulatory properties of mesenchymal stem cells: a review based on an interdisciplinary meeting held at the Kennedy Institute" potx

Ngày tải lên : 09/08/2014, 08:23

... MSCs inhibit the division of stimulated T cells by preventing their entry into the S phase of the cell cycle and by mediating an irreversible G0/G1 phase arrest [2] MSCs also induced arrest of ... systemic administration [53] They contribute to myofibres and to long-term persisting functional satellite cells; furthermore, they restore sarcolemmal dystrophin when they are injected into the ... immunonogenicity of MSCs in nonimmunocompromised animals, on the optimal source, on the timing and number of cells to be infused, and on the location and determinants of cell homing are needed It would...

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Báo cáo y học: "Microenvironmental changes during differentiation of mesenchymal stem cells towards chondrocytes" ppt

Ngày tải lên : 09/08/2014, 10:20

... members of the integrin family The integrin family of CAMs are transmembrane glycoproteins, composed of α and β subunits and their combination determines ligand specificity The patterns of integrin ... in the analysis of the data PL-P helped in the analysis of the data FC procured the samples for the isolation of MSCs PC participated in the analysis of the data CJ participated in the design of ... integrins α1, α2, α3, α5, α6, α10, αv, β1, β3 and β5 [52] Integrin α5β1 is the most prominently expressed integrin in chondrocytes and integrin α10β1 is the dominant collagenbinding integrin during...

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s Membrane proteomic analysis of pancreatic cancer cells docx

Ngày tải lên : 10/08/2014, 05:21

... patterns of membrane proteins from the two cell lines (Figure 1) The proteins showing differential levels include cadherins, catenin, integrins, galectins, annexins, collagens and many others, ... multiple steps and interactions between tumor cells and the host These include detachment of tumor cells from the primary tumor, intravasation into lymph and blood vessels, survival in the circulation, ... result from numerous mutations of the gene, hindering the matching of peptides based on molecular weight Some of the proteins identified from the current study may be further verified in clinical...

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báo cáo khoa học: " The HPB-AML-I cell line possesses the properties of mesenchymal stem cells" ppt

Ngày tải lên : 10/08/2014, 10:20

... hydrocortisone, and indomethacin, but not troglitazone, an increase in the number of lipid droplets was observed in these cells [12] In view of these features, we further investigated the possibility of HPB-AML-I ... Cytogenetic analysis was performed according to the standard protocols The karyotype was determined by G-banding using trypsin and Giemsa (GTG) [16] to examine 50 cells The best metaphase was then photographed ... cytochemical staining for this protein (Figure 4K) On the other hand, cytochemical staining resulted in positive staining for alkaline phosphatase in the cytoplasm of differentiated HPBAML-I cells (Figure...

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báo cáo khoa học: " Efficacy of Mesenchymal Stem Cells in Suppression of Hepatocarcinorigenesis in Rats: Possible Role of Wnt Signaling" doc

Ngày tải lên : 10/08/2014, 10:21

... mimicking the one of resident cells in the host tissue Insults trigger the release of chemokines from the endothelium inducing adhesion and migration of circulation BMDC into the liver parenchyma The ... colleagues[66], investigated the malignant potential of hepatic stem cells derived from the bone marrow in a mouse model of chemical hepatocarcinogenesis, their results suggested that hepatic stem cells derived ... development of HCC seems to be the abnormal regulation of the transcription factor b-catenin, a key component of the Wnt signaling pathway [58] In the normal state, the binding of members of a family of...

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báo cáo khoa học: " Transcriptomic analysis of pluripotent stem cells: insights into health and disease" potx

Ngày tải lên : 11/08/2014, 12:21

... chromatin-binding proteins and modifiers, proteinmodifying factors, and chromatin assembly proteins Importantly, knockdown of Oct4 protein levels is known to cause the loss of co-binding activity of ... identification of long non-coding RNA (lncRNA) [12] and long intergenic non-coding RNA (lincRNA) [13,14] Sequence-based transcriptomic analysis on the other hand involves direct sequencing of the cDNA Initially, ... determine the gene targets of these repro gramming miRNAs Expression profiling of ESCs and iPSCs The question of whether pluripotent iPSCs truly resemble ESCs is an actively debated and evolving...

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Báo cáo y học: "Obstructive apneas induce early activation of mesenchymal stem cells and enhancement of endothelial wound healing" ppsx

Ngày tải lên : 12/08/2014, 11:22

... initial and final limits of the wound The closure index was computed as the increase in the wound's endothelial area, normalized to the mean increase in the case of the control serum Statistical analysis ... to the obstructive stimulus would be sensed by the MSC in the bone marrow (and in other tissues which are also reservoirs of these cells) and would induce their mobilization from their original ... healing test showing the wound at the beginning of the experiment (left) and 24 h after the culture medium was replaced by apneic rat serum (right) Red and yellow lines indicate the initial and...

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Báo cáo y học: " Curcumin mediated suppression of nuclear factor-κB promotes chondrogenic differentiation of mesenchymal stem cells in a high-density co-culture microenvironment" pdf

Ngày tải lên : 12/08/2014, 14:22

... combination of both (1) lead to the production and accumulation of high levels of pro-inflammatory cytokines (2) in the joint These trigger the production of additional pro-inflammatory cytokines ... this inflammatory and catabolic cascade and demonstrated that curcumin (6) has the capacity to block the action of pro-inflammatory cytokines in the joint thus disrupting the inflammatory cycle inhibit ... positive chondrogenic signaling However, interaction of curcumin and TGF-β signaling in chondrocytes is an interesting point and will require further detailed investigations In the present study we...

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Báo cáo y học: " Influence of hypoxia on the domiciliation of Mesenchymal Stem Cells after infusion into rats: possibilities of targeting pulmonary artery remodeling via cells therapies " doc

Ngày tải lên : 12/08/2014, 18:20

... harvested from 6-weeks-old Wistar rats and the adherent soft tissue was removed The proximal and distal ends of the femur were excised at a level just into the beginning of the marrow cavity Whole marrow ... Isotopic labeling and Indium-111 labeled MSCs intravenous infusion The cells were incubated with 11 1In- oxine (37 MBq/106 cells) and incubated for 60 minutes as previously described [11] The radiolabeled ... 8B) confirming the presence of GFP -cells into the lungs To extract and culture the engrafted GFP-labeled cells from lungs following the same protocol of three-week cell injection and hypoxic...

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Characterization of the secretion of mesenchymal stem cells and its relevance to cardioprotection

Ngày tải lên : 10/09/2015, 08:42

... that the mortality rate of AMI in Germany reduced from 16.2% in 1994 to 9.9% in 2002 in tandem with the increasing use of reperfusion therapy6 The efficacy of reperfusion therapy has led to increasing ... third of the publications in the regenerative medicine field29 The rationale for the use of stem cells to repair cardiac tissues was based on the hypothesis that these cells could differentiate into ... support the stem cell crypt in the intestine65; protect against ischemic renal59,60 and limb tissue injury62; support and maintain hematopoiesis63; support the formation of megakaryocytes and proplatelets66;...

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Transplantation of mesenchymal stem cells for the treatment of parkinsons disease in a mouse model

Ngày tải lên : 11/09/2015, 09:05

... microglial cells in the SNc of MPTP-treated mice, real-time RT-PCR did not find mRNA expression of MBL in the brain parenchyma, suggesting the infiltration of MBL from the circulation into the brain ... stain positively for ubiquitin and α-synuclein.Ubiquitin is a protein involved in the degradation of cytoplasmic proteins The function of α-synuclein is still not clear In the healthy brain, ... also be involved in the pathogenesis of PD (Qin et al., 2007) The cytokines freely diffuse from the blood into the brain parenchyma where they may then interact with microglia In addition to the...

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Recruitment of mesenchymal stem cells to injury sites

Ngày tải lên : 13/10/2015, 15:54

... adhesion with the endothelium via integrins The final step of the process involves the leukocyte transmigrating across the endothelium into the interstitium 1.2.2 Current understanding of MSC recruitment ... their counter ligands expressed on endothelial cells The final step involves the extravasation of the leukocytes across endothelial tight junctions into the interstitium During the onset of inflammation ... They showed that co-culturing of MSC with embryonic stem (ES) cell -derived endothelial cells resulted in the MSC integrating into the endothelial monolayer, which was presumed to be part of the...

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Lipidomics of mesenchymal stem cells undergoing adipogenesis

Ngày tải lên : 16/10/2015, 15:35

... leaflet of the plasma membrane of cells (Vance, 2008) and is a precursor to the biosynthesis of PE During apoptosis, PS moves from the inner leaflet to the outer leaflet of the cell The presence of ... been the most well-studied As tissue specimen from these areas are easily available and the techniques of isolating MSC from these tissues and in vitro expansion and maintenance of these cells ... al., in their review on prostaglandins and leukotrienes, have illustrated the actions of prostaglandins in eliciting inflammatory, algesic and pyrogenic response, depending on the location of action;...

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báo cáo hóa học:" Revision of failed hip resurfacing to total hip arthroplasty rapidly relieves pain and improves function in the early post operative period" pdf

Ngày tải lên : 20/06/2014, 04:20

... components in the bearing couple when revising for this indication While it is too early to comment on the clinical outcomes of these prostheses, the main determinants of success in these patients ... absence of infection Cystic lesions were noted behind the acetabular component in cases but the cup was grossly loose in only one of these There was evidence of gross collapse of a segment of the ... femoral head (evidenced by softening of the bone at the margin of the prosthesis) in one patient Pre operative Oxford, Harris and WOMAC hip scores were 39.1, 36.4 and 52.2 respectively Post operative...

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