chondrocytes and mesenchymal stem cells in cartilage tissue engineering and in the regenerative therapy of joint diseases

Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity

Ngày tải lên : 11/09/2015, 09:16

... 2001;Function and interactions of integrins Cell Tissue Res (3):285-298 41 Mould AP, Askari JA, Aota SI, et al 1997;Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory ... 2007 ;In vitro enhancement of collagen matrix formation and crosslinking for applications in tissue engineering: a preliminary study Tissue Eng (2):385391 37 Qian L, Saltzman WM 2004;Improving the ... isolation and expansion Cytotherapy 75 Du F, Zhou Z, Mo ZY, et al 2006;Mixed macromolecular crowding accelerates the refolding of rabbit muscle creatine kinase: implications for protein folding in physiological...

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Differentiation and derivation of lineage committed chondroprogenitors and chondrogenic cells from human embryonic stem cells for cartilage tissue engineering and regeneration

Ngày tải lên : 11/09/2015, 09:57

... characterized by the breakdown of the joint s cartilage Cartilage is the part of the joint that cushions the ends of the bones and allows easy movement of joints The breakdown of cartilage causes the bones ... differentiation of ESCs and for cartilage tissue engineering Some of the biomaterials and their associated outcomes in ESC-based cartilage tissue engineering and regenerative medicine are summarized in Table ... 2.3.4.1 Cartilage tissue engineering using hydrogels Cartilage tissue engineering using hydrogels enables the delivery of cells and bioactive agents, including growth factors, peptides and ligands...

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Báo cáo y học: "Therapeutic potential of human umbilical cord mesenchymal stem cells in the treatment of rheumatoid arthritis" pps

Ngày tải lên : 12/08/2014, 15:22

... cells of synovial joints, involved in pannus formation, and are key players in the destruction of cartilage and bone in RA joint [10] The ability of FLSs to stimulate both inflammation and tissue ... cytokine for Tregs, and plays a key role in the control of self-antigen-reactive T cells in vivo [38] The upregulation of IL-10 is in line with the induction of Tregs in vitro and in vivo in our ... both inflammation and tissue damage One of the critical mediators of inflammation in RA is the proinflammatory cytokine IL-6 Interestingly, the UC-MSCs could downregulate the IL-6 production in the...

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Báo cáo khoa học: "Transplantation of canine umbilical cord blood-derived mesenchymal stem cells in experimentally induced spinal cord injured dogs" pot

Ngày tải lên : 07/08/2014, 23:22

... ytisrevinU lanoitaN luoeS fo ’slaminA yrotarobaL fo esU dna eraC eht rof ediuG‘ eht fo senilediug eht ot gnidrocca demrofrep saw noitagitsevni sihT yduts yrujni droc lanips latnemirepxe eht rof desu ... erew sgod ehT ICS eht retfa keew a demrofrep saw sCSM-BCU eninac fo noitatnalpsnarT :FSCGhmr fo noitcejni dna sCSM-BCU eninac fo noitatnalpsnarT noitcejni eht rof noitulos enilas fo lµ 051 ni 601 ... ilumits cinegotim cificepsnon ro ralullec yb decudni noitarefilorp etycohpmyl-T sserppus sllec lamorts worram enob namuH MA innaiG ,S itnasirG ,P iccuettaM ,DP inognoL ,M isenaliM ,M ingaM ,C...

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Báo cáo khoa học: "Functional recovery and neural differentiation after transplantation of allogenic adipose-derived stem cells in a canine model of acute spinal cord injury" potx

Ngày tải lên : 07/08/2014, 23:22

... during in vitro culture [4] These findings suggested the presence of mesenchymal stem cell-like cells according to the standard criteria for MSCs from the International Society for Cellular Therapy ... hematoxylin and eosin, and then with combined Luxol fast blue and cresyl violet to identify myelin and nerve cells [10] Percentages of myelinated areas in damaged spinal cords were calculated using the ... effective in canine SCI model, there was little histological evidence of spinal cord tissue regeneration [22] In this study, we examined whether canine ASCs could survive and integrate into neural cells...

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Báo cáo y học: "Mesenchymal stem cells in arthritic disease" pot

Ngày tải lên : 09/08/2014, 13:22

... property of MSCs, make MSCs the cell type of choice for articular cartilage tissue engineering that aims to replace and regenerate the diseased structure in joint diseases In addition, their immunomodulatory ... detected in joints of treated mice, suggesting that the injected MSCs did not restore tissue integrity by mechanisms of direct tissue repair At the end of the experiment, cells were not evident in ... burden of degenerative joint diseases through their application in the form of replacement tissue as well as local or systemic cell therapy Competing interests The authors declare that they have...

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báo cáo khoa học: " Efficacy of Mesenchymal Stem Cells in Suppression of Hepatocarcinorigenesis in Rats: Possible Role of Wnt Signaling" doc

Ngày tải lên : 10/08/2014, 10:21

... mimicking the one of resident cells in the host tissue Insults trigger the release of chemokines from the endothelium inducing adhesion and migration of circulation BMDC into the liver parenchyma The ... signaling in tumor tissues by assessing the gene expression profile of some of the Wnt signaling target genes:cyclin D, PCNA, survivin, b-catenin Methods Ninety albino female rats inbred strain (Cux1: ... development of HCC seems to be the abnormal regulation of the transcription factor b-catenin, a key component of the Wnt signaling pathway [58] In the normal state, the binding of members of a family of...

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Báo cáo y học: " Mesenchymal stem cells in autoimmune diseases: hype or hope" ppt

Ngày tải lên : 12/08/2014, 14:21

... incomplete understanding of the mechanisms underlying these divergent results, and inspired by positive reports on the use of bone marrow-derived MSCs in the outcome of GVHD and transplantation ... that the endeavors will eventually pay off, but more experience with the use of MSCs in the setting of GVHD can help guide their use for rheumatic diseases, thereby certifying or revoking their therapeutic ... Concluding remarks The mechanisms underlying the possible in vivo immunomodulatory effects of MSCs remain a critical and unresolved question By comparing side by side the effects of MSCs in vitro and...

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Báo cáo y học: " Curcumin mediated suppression of nuclear factor-κB promotes chondrogenic differentiation of mesenchymal stem cells in a high-density co-culture microenvironment" pdf

Ngày tải lên : 12/08/2014, 14:22

... combination of both (1) lead to the production and accumulation of high levels of pro-inflammatory cytokines (2) in the joint These trigger the production of additional pro-inflammatory cytokines ... this inflammatory and catabolic cascade and demonstrated that curcumin (6) has the capacity to block the action of pro-inflammatory cytokines in the joint thus disrupting the inflammatory cycle inhibit ... curcumin action [35,36] As β1-integrins are highly expressed in developing and adult cartilage, it is possible that the mechanism of action of curcumin in MSCs and chondrocytes might involve the...

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Báo cáo khoa học: " An immunohistochemical study of chromogranin A and Sp-1 immunoreactive cells in the gastrointestinal tract of ovariectomized rats" pdf

Ngày tải lên : 07/08/2014, 18:21

... htiw demrofrep saw noitcaer cificepsnon fo gnikcolB ]13[ dohtem )PAP( esadixorep itna-esadixorep eht htiw deniatsonummi dna detardyher ,deziniffaraped saw noitces evitatneserper hcaE gniniats lacimehcotsihonummI ... etanobracylop rep detacolla erew slaminA syad rof noitazitamilcca retfa desu erew )napaJ ,reviR selrahC ;tpiecer nopu dlo kw-6( tar elamef )DS( yelwaD-eugarpS ytnewT o slamina latnemirepxE sdohteM dna ... lanoitubirtsid eht ,hguohtlA ]8,3[ sllec enircodneoruen lla fo ”srekram“ nommoc yltnacifingis a ,stneitap aitreni cinoloc nI slamina fo sutats evitsegid ot detaler ylraelc erew sllec RI-GC fo segnahc eht...

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Báo cáo y học: "Stem cells in the genomic age" docx

Ngày tải lên : 14/08/2014, 16:21

... developed in his group This, he told us, is just the beginning of an ambitious and far-reaching transcriptional profiling project As other labs around the world develop reporter lines for their favorite ... into an oocyte or fused with an ES cell, the underlying mechanisms remain obscure Using the ES fusion system, Smith has found an unexpected role for the homeodomain-containing protein Nanog in ... process By boosting levels of Nanog it is possible to increase the efficiency of nuclear reprogramming up to 100-fold in cell fusions between ES cells and neural stem cells, and further experiments...

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Báo cáo y học: "Enhanced effector function of cytotoxic cells in the induced sputum of COPD patients" doc

Ngày tải lên : 12/08/2014, 11:22

... function within the airways Therefore, in this study we have extended the findings of our previous study to investigate, within induced sputum, the number and cytotoxic function of the three main classes ... shown) There was no difference in the proportions of CD56dim CD16+ NK cells and CD56 brightCD16- NK cells expressing only perforin and no granzyme B Examining the proportion of cells expressing ... peripheral cells does not necessarily reflect changes in the airway The first step of assessing the potential importance of these cells in the inflammatory process would be to assess their numbers and...

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Báo cáo y học: " In situ detection of Gag-specific CD8+ cells in the GI tract of SIV infected Rhesus macaques" pptx

Ngày tải lên : 12/08/2014, 23:23

... localization of virus specific CD8+ T cells may prove useful in the study of the pathogenesis of SIV infection and the role vaccines and immunotherapy may play in altering the CD8+ T cell response in the ... positive cells were seen in the junction between the follicle and the extra follicular area, and in the extra follicular area (Fig 5D and 5E), indicating that some of these cells are able to enter the ... specific cells in frozen tissue The use of SIV specific APC tetramers for in situ staining traditionally requires a two-step enhancement methodology and the use of fresh tissue, as staining in frozen...

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english professionl titles in the management system of joint-stock companies and their vietnamese equivalents = từ chỉ chức danh thuộc hệ thống quản lý của công ty cổ phần trong tiếng anh

Ngày tải lên : 02/03/2015, 14:29

... American and Vietnamese Joint- Stock Companies, including the definition of a joint- stock company and the illustration of common company structures 2.1 Definition of American and Vietnamese joint- stock ... various forms of financial reporting Accounting Manager/ Chief Accounting Officer: An accounting manager may work under the guidance of the CFO and is in charge of directing and coordinating the daily ... primary purpose of the study is to look for the intrinsic meanings of the words denoting common professional titles in management system of American joint- stock companies and their Vietnamese...

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Báo cáo y học: "An in vitro study investigating the survival and phenotype of mesenchymal stem cells following injection into nucleus pulposus tissue" pot

Ngày tải lên : 09/08/2014, 01:22

... and 3,3-diaminobenzidine disclosure) for aggrecan and the staining intensity increased with time post injection (Figures and 5b) Aggrecan matrix staining within the vicinity of the injected cells ... performed the type X collagen and Alizarin red staining, and participated in interpretation of the data AJF participated in interpretation of the data and contributed to the preparation of the final ... following injection, and the intensity of the matrix staining increased with time in culture (Figures and 5b) Weak staining for type II collagen was observed within a small number of cells in monolayer...

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Báo cáo y học: "Notochordal conditioned media from tissue increases proteoglycan accumulation and promotes a healthy nucleus pulposus phenotype in human mesenchymal stem cells" pot

Ngày tải lên : 12/08/2014, 15:23

... number of in vitro and in vivo studies [10-19] The interaction between MSCs and cells of the native IVD, including the adaptation of MSCs to the IVD microenvironment, enhanced MSC metabolism and ... In a number of species, including humans, during growth and aging, the NCs populating the NP disappear and are replaced by chondrocytic NP cells [33] The NP of some species retain notochord cells ... the disc, the fibrochondrocytes that populate and maintain the annulus fibrosus (AF) and the more chondrocytic cells in the nucleus pulposus (NP) The NP cells are often described as being “chondrocyte-like”...

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Vascularised bone tissue engineering endothelial progenitor cells and human mesenchymal stem cells coculture in 3d honeycomb scaffolds and the effect of bi rotational bioreactor and hypoxic microenvironment 1

Ngày tải lên : 09/09/2015, 10:16

... SH Teoh Cellular interactions of endothelial progenitor cells and mesenchymal stem cells for vascularised bone tissue engineering Tissue Engineering and Regenerative Medicine International Society ... Limitations in Bone Tissue Engineering Since the introduction of BTE over the past two decades, there has been an exponential increase in the number of investigations in the area of BTE, with ... continuous hypoxic exposure for the culture of human fetal mesenchymal stem cells in bone tissue engineering applications 3rd Tissue Engineering and Regenerative Medicine International Society (TERMIS)...

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Báo cáo khoa học: A comparative analysis of the transcriptome and signal pathways in hepatic differentiation of human adipose mesenchymal stem cells doc

Ngày tải lên : 30/03/2014, 04:20

... pivotal factor in determining stem cell transdifferentiation Discussion AT-MSCs may be good candidates as stem cells for cell transplantation and tissue engineering in regenerative medicine, as a ... distinct narrow time interval of transcription initiation [25], and regulate transactivation of several endodermand hepatocyte-speciﬁc factors, including transthyretin, albumin and tyrosine aminotransferase ... expressed in the human liver [20], and its product is involved in the metabolism of 45–60% of the drugs metabolized by cytochrome P450 proteins [21,22] Our data indicate that the amount of CYP3A4...

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Báo cáo hóa học: " Adipose-derived mesenchymal stem cells markedly attenuate brain infarct size and improve neurological function in rats" pptx

Ngày tải lên : 18/06/2014, 16:20

... transplanted cells into the host tissue [3] Interestingly, while stem cell therapy, including bone marrow-derived mesenchymal stem cells [15-17], embryonic stem cells [14] and endothelial progenitor cells ... value of n = 3) on day 14 after cell culturing showed the CD29 + and CD90+ cells were the highest population of stem cells Spindle-shaped morphological feature of the stem cells were shown in the ... by administration of ADMSCs may be partially through reinforcing SDF-1α chemokine expression in the BIA Beside the findings of upregulated expressions of CXCR4 and SDF-1α in BIA, ADMSC therapy...

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Báo cáo hóa học: "Early combined treatment with sildenafil and adipose-derived mesenchymal stem cells preserves heart function in rat dilated cardiomyopathy" doc

Ngày tải lên : 18/06/2014, 16:20

... preservation of heart function in DCM rats Accordingly, our finding strengthens the finding of previous study [20] Another important finding in the current study is that combined therapy with ADMSCs and ... groups and These findings indicate that the expression of cytochrome C, an index of energy supply and storage in mitochondria, was notably lower in group than in groups and The increase in cytosolic ... superior to either therapy alone in abrogating the progression of DCM Synergic Action of Early Combined Therapy with Sildenafil and ADMSCs in Preservation of LV Function in Rat DCM Lack of Evidence...

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