chaotization of human systems by technical electromagnetic fields
Tài liệu Báo cáo Y học: Study of substrate specificity of human aromatase by site directed mutagenesis pdf
Ngày tải lên :
21/02/2014, 15:20
... affinity of the substrates, particularly testosterone, and of the inhibitors by disfavoring the interaction of the pyridyl group with the heme This could be explained by the impact of the position of ... report of three isoforms of porcine aromatase, encoded by distinct genes [26] and the study of their catalytic differences [27,28] also highlight the need to understand which residues of the ... that an increase of the hydrophobic character of this part of the human active site modified the aromatization efficiency of androstenedione but increased the inhibition potency of MR 20814 and...
- 13
- 506
- 0
Báo cáo Y học: Concentration-dependent reversible activation-inhibition of human butyrylcholinesterase by tetraethylammonium ion pptx
Ngày tải lên :
24/03/2014, 03:21
... hydrolysis of BTC by this enzyme obeyed Michaelis–Menten kinetics In order to investigate the kinetics of this mutant more closely, we measured the hydrolysis of BTC catalyzed by the W82A mutant, by ... concentration of the product was formed The stock solutions of the two enzymes were prepared by dilution of the aliquots with the same amount of buffer The mixture was prepared by mixing together ... built model of human BuChE (CODE P06276) from Swiss-Model, an automated protein modeling Fig pS curves for the hydrolysis of butyrylthiocholine catalyzed by the wild-type, by various human butyrylcholinesterase...
- 8
- 321
- 0
Báo cáo khoa học: Catalytic activation of human glucokinase by substrate binding – residue contacts involved in the binding of D-glucose to the super-open form and conformational transitions ppt
Ngày tải lên :
30/03/2014, 04:20
... wild-type hGK ( ) by increasing concentrations of Glc The solid lines represent the fit of the data to two hyperbola as obtained by nonlinear regression analyses, giving Kd values of 4.8 ± 0.1 mM ... purification of recombinant hGK The wild-type and mutant forms of hGK isoform were expressed as GST fusion proteins Expression in Escherichia coli (BL21) cells at 28 °C and purification of proteins by glutathione ... cleavage for h at °C by factor Xa at a protease to substrate ratio of : 25 (by mass) Glutathione and salts were removed by size exclusion chromatography (Sephadex G-25), followed by glutathione Sepharose...
- 15
- 522
- 0
báo cáo hóa học:" Research Article Real-Time Multiview Recognition of Human Gestures by Distributed Image Processing" pdf
Ngày tải lên :
21/06/2014, 20:20
... computed by dividing “the rate of correct answers” by “the rate of correct answers” plus “the rate of wrong answers.” “The rate of correct answers” refers to the ratio of the number of correct ... frame rate of gesture recognition systems depends on (1) duration of each gesture, (2) number of gesture classes, and (3) performance of the implemented system In addition, recognition systems must ... human gestures with more flexibility, protocol learning is conducted The purpose of protocol learning is to let the system focus on visual features of greater significance by using a sequence of...
- 13
- 257
- 0
Báo cáo hóa học: " Research Article Nonexpansive Matrices with Applications to Solutions of Linear Systems by Fixed Point Iterations Teck-Cheong Lim" docx
Ngày tải lên :
21/06/2014, 20:20
... eigenvalue of B Proof As in the proof of Theorem 2, a and b are equivalent For < μ < 1, denote μI − μ B by B μ Suppose now that a holds Let λ be an eigenvalue of B Then μ 1− μ λ is an eigenvalue of ... The index of the eigenvalue of Q−1 A is the R Q−1 A −1 index of eigenvalue of B I − Q A Thus by Lemma 5, Cn Q−1 R A ⊕ N A For every vector v ∈ Cn , let v r and v n denote the component of v in ... eigenvalue of B μ By Theorem |μ − μ λ| ≤ for every < μ < and hence |λ| ≤ If is an eigenvalue of B, then it is also an eigenvalue of B μ By Theorem 2, the index of 1, as an eigenvalue of B μ , is Since...
- 13
- 291
- 0
The use of english idioms related to parts of human body by the third year students at faculty of english, hanoi open university
Ngày tải lên :
24/06/2016, 21:21
... lower part of human body The tables 4, and below show the results of the questions of three groups respectively 24 Table 4: the results of the questions related to the upper part of human body ... idioms related to parts of human body PART DEVELOPMENT Chapter Literature review of idioms 1.1 Definition of idiom English conversation in human daily speech is full of expressions or phrases ... presented the results of the questions of the three groups respectively Table 1: the results of the questions related to the upper part of human body Question number Number of students 36 15 21...
- 41
- 389
- 0
Control of Membrane Fouling by Coagulant and Coagulant Aid Addition in Membrane Bioreactor Systems
Ngày tải lên :
05/09/2013, 10:15
... substances (EPS) of the mixed liquor were extracted by steam treatment at 105 oC for 30 minutes (Nakajima and Mishima, 2005) For the SMP
- 11
- 575
- 0
Tài liệu Báo cáo khoa học: Autolytic activity of human calpain 7 is enhanced by ESCRT-III-related protein IST1 through MIT–MIM interaction pptx
Ngày tải lên :
18/02/2014, 04:20
... they were probably derived by limited digestion with other cellular proteases, and not by autolysis of mGFP–calpain Enhancement of autolysis of mGFP– calpain 7–Strep by GST–MIM in vitro As we ... analyzed by WB with mAb against GFP to detect proteolysed fragments of mGFP–calpain 7–Strep Bands of full-length WT and C290S are indicated by the arrow, and those of 30 K are indicated by the ... Enhancement of calpain autolysis by IST1 Y Osako et al membranes [42] ALIX is recruited to plasma membranes by Gag proteins of HIV-1 and equine infectious anemia virus through binding of the V domain of...
- 15
- 505
- 0
Tài liệu Báo cáo khoa học: Reversible tetramerization of human TK1 to the high catalytic efficient form is induced by pyrophosphate, in addition to tripolyphosphates, or high enzyme concentration ppt
Ngày tải lên :
18/02/2014, 12:20
... Discussion Identification of the group inducing tetramerization of human TK1 Human TK1 has 234 amino acids and a subunit size of 25.5 kDa [18] Several reports have shown by gel filtration that native ... regulation of human TK1 Fig Effect of nucleotides and polyphosphates on oligomerization of native hTK1 Approximately 10 ng native TK1 purified from human lymphocytes in a total volume of 200 lL ... Enzymatic regulation of human TK1 Impact of enzyme concentration on the oligomerization of hTK1 Fig Effect of orthophosphate and di- and tri-polyphosphates on oligomerization of native hTK1 hTK1...
- 10
- 647
- 0
Tài liệu Báo cáo khoa học: Impact of the native-state stability of human lysozyme variants on protein secretion by Pichia pastoris doc
Ngày tải lên :
19/02/2014, 08:20
... five years, two novel variants of human lysozyme, F57I and W64R, have been identified by the detection of heterozygous, single-base mutations in the lysozyme gene of patients suffering from hereditary ... lysozyme yielded 8.3 mgÆL)1 The yields of the F57I and W64R variants were therefore lower, by factors of 200 and 30, respectively, relative to that of WT human lysozyme in these experiments Under ... similar extent Of particular interest from the point of view of amyloid disease is the characterization of the two new mutational variants associated with clinical disease Analysis of both the F57I...
- 10
- 577
- 0
Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp docx
Ngày tải lên :
20/02/2014, 02:21
... produced by agmatine hydrolysis, in studying the inhibitory effect of agmatine on arginine hydrolysis, reactions were followed by measuring the formation of ornithine, determined by the method of Chinard ... release of Mn2+B by EDTA during preparation of a substrate complex of Bacillus caldovelox arginase for crystallographic analysis [32] According to our present results, the catalytic activity of the ... resistant to inhibition by the substrate analog As an example, only about 25% inhibition of the wild-type and mutant enzymes was pro- Fig Catalytic activity of the Asn149Asp variant of human arginase...
- 9
- 651
- 0
Tài liệu Báo cáo Y học: Progressive apoptosis in chorion laeve trophoblast cells of human fetal membrane tissues during in vitro incubation is suppressed by antioxidative reagents pptx
Ngày tải lên :
22/02/2014, 07:20
... glucocorticoid, those of NF-kB and TNFa were at the same level as in the absence of glucocorticoid, those of IL-1b and IL-6 were higher than in the absence of glucocorticoid, and those of Cu/Zn-SOD, ... presence of a lower concentration of glucocorticoid during incubation suppressed the progression of trophoblast cell apoptosis in the tissue, and that apoptosis of primary cultured human chorion ... trophoblast cells induced by TNFa was also suppressed by this lower concentration of glucocorticoid [1] These previous results suggested that the expression of mRNA of inflammatory cytokines and/or...
- 8
- 477
- 0
Mobility of Human Resources and Systems of Innovation pdf
Ngày tải lên :
06/03/2014, 05:22
... of networks of innovation.9 This section has discussed five types of mobility: that of national systems, regional systems, sectoral systems, technological systems and socio-economic systems It ... urban, sectoral and technological systems of innovation may be distinguished, the systems -of- innovation approach originated in an examination of national systems of innovation (NSIs), as Lundvall ... mobility of highly skilled personnel In so doing, it has raised some issues of particular importance for analysing mobility of skilled South African human resources By way of conclusion, a few of the...
- 82
- 359
- 0
Báo cáo khoa học: Catalytic digestion of human tumor necrosis factor-a by antibody heavy chain pot
Ngày tải lên :
06/03/2014, 22:21
... with human TNF-b at all Sequences and steric conformation of ETNF-6 mAb Sequencing of the cDNA of the variable region of ETNF-6 mAb was performed, and this was followed by molecular modeling of ... Catalytic digestion of TNF-a by the antibody subunit E Hifumi et al Results Immunological features of the antibodies By normal cell fusion [21] after the immunization of human TNF-a (hTNF-a) ... After a reaction time of 47.2 h, the amount of TP41-1 at a retention time of 11 decreased, because of fragmentation The fragment was observed at a retention time of 13 (indicated by an arrow) as a...
- 10
- 491
- 0
Báo cáo khoa học: Mechanisms and kinetics of human arylamine N-acetyltransferase 1 inhibition by disulfiram potx
Ngày tải lên :
07/03/2014, 02:20
... of NAT1 could be reversed by reducing agents As shown in Table 1, after inhibition by DS, NAT1 activity could not be restored by physiological concentrations of reduced glutathione (GSH) or by ... constant for the inhibition of NAT1 by DS (ki) was deduced from the slope of kobs as a function of the DS concentration [DS], and was · 104 m)1Æmin)1 The order of the reaction of DS with NAT1 (n) can ... reaction of cisplatin with the enzyme, thus supporting the suggestion that NAT1 could be an in vivo target of DS The human NAT2 isoform shares a similar structure and mechanism of action to human...
- 9
- 456
- 0
Báo cáo khoa học: ` Inhibition of human ether a go-go potassium channels by 2+ Ca ⁄calmodulin binding to the cytosolic N- and C-termini potx
Ngày tải lên :
07/03/2014, 12:20
... 1–206 in steps of 3) and C-terminal (amino-acids 480–962 in steps of and amino-acids 645–895 in steps of 2) cytosolic domains of hEAG1 Binding of BODIPY FL-labeled hCaM in the presence of 25 lm free ... mechanism of CaM-mediated closure of hEAG1 channels is far from understood mainly because of the lack of structural information on the cytosolic channel domains in complex with CaM Initiated by the ... with the C-terminal domain of Ca2+-free CaM, i.e apoCaM The activation of these channels is mediated by conformational changes in the CaM ⁄ SK2 complex induced by binding of at least one Ca2+ ion...
- 13
- 500
- 0
Báo cáo khoa học: Functional significance of five noncanonical Ca2+-binding sites of human transglutaminase 2 characterized by site-directed mutagenesis potx
Ngày tải lên :
16/03/2014, 03:20
... adhesive functions of cells [11] The actual enzymatic activity of TG2 is determined by its structural state induced by the types and amounts of bound ligands [12] The X-ray structure of TG2 in its ... presence of mM Ca2+ (D) Inhibition of residual transglutaminase activity of recombinant TG2s by GTP, using the microtiter plate method Activity is shown as a percentage of the activity of wild-type ... binding of celiac autoantibodies to TG2 is influenced by the presence or absence of Ca2+ in the case of guinea pig TG2 [27] Therefore, we also examined the effect of Ca2+ and GDP on the binding of...
- 14
- 378
- 0
Mobility of Human Resources and Systems of Innovation pptx
Ngày tải lên :
16/03/2014, 11:20
... of networks of innovation.9 This section has discussed five types of mobility: that of national systems, regional systems, sectoral systems, technological systems and socio-economic systems It ... urban, sectoral and technological systems of innovation may be distinguished, the systems -of- innovation approach originated in an examination of national systems of innovation (NSIs), as Lundvall ... mobility of highly skilled personnel In so doing, it has raised some issues of particular importance for analysing mobility of skilled South African human resources By way of conclusion, a few of the...
- 82
- 356
- 0
Báo cáo khoa học: Mutational analysis of substrate recognition by human arginase type I ) agmatinase activity of the N130D variant pot
Ngày tải lên :
16/03/2014, 12:20
... inhibitor of agmatine and arginine hydrolysis by the N130D variant, with Ki values of 1.3 ± 0.2 mm and 1.5 ± 0.1 mm, respectively The Table Kinetic properties of the wild-type and N130D variants of human ... indicated by the numbers on the right side The migration of the protein markers is indicated by pencil marks on the membrane The arrow indicates the position of the wild-type and N130D variants of human ... efficiency of human arginase I are markedly altered by replacement of Asn130 with aspartate In addition to supporting the importance of Asn130 in substrate binding and discrimination by arginase...
- 7
- 303
- 0
Báo cáo khoa học: Targeting mechanism of the retinoblastoma tumor suppressor by a prototypical viral oncoprotein Structural modularity, intrinsic disorder and phosphorylation of human papillomavirus E7 doc
Ngày tải lên :
22/03/2014, 21:20
... thermodynamic understanding of the mechanisms of action of the Rb protein is far behind that of p53, the keeper of the genome, mutated in most cancers and targeted by the same DNA tumor viruses ... purified by RP-HPLC The phosphorylated E7(16-40) peptide was obtained by incorporation of phosphoserine, instead of serine, in the synthesis The relative molecular mass of each peptide was confirmed by ... in retinoblastoma tumors, 50 of which are missense mutations [2,3] The tumor suppressor function of the Rb protein is underscored by its mutation in a broad range of human tumors [4] The most extensively...
- 16
- 404
- 0