cell based assays for regulators of tau biology
Development of human stem cell based model for developmental toxicity testing
Ngày tải lên :
09/09/2015, 08:18
... epithelial cells lose their cell polarity and cell- cell adhesion and become migratory and invasive mesenchymal cells It is important for early cell differentiation and collective cell migration ... (a, b) (c) Quantification of ppMLC expression at the apical, lateral and basal cellular domains of T+ cells at the periphery of µ P-hPSC colonies Data are average ± s.e.m of images *p
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Báo cáo hóa học: " Carbon composite micro- and nano-tubes-based electrodes for detection of nucleic acids" pot
Ngày tải lên :
21/06/2014, 03:20
... suitable tool for detection of nucleic acids We show the successful application of modern nano-technologies not only for detecting of nucleic acids but also for distinguishing of all bases signals ... Therefore, the versatility of the electrode was tested Cyclic voltammetry was used for the detection of two nucleic acids’ samples mentioned above and the basic electrochemical behaviour of nucleic ... http://www.nanoscalereslett.com/content/6/1/385 A Page of B Figure Preparation and characterization of MWCNTs (A) Set-up of the apparatus for PECVD MWCNTs direct deposition (according to [8]) (B) Surface-enhanced micrographs of the electrodes...
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báo cáo hóa học:" Research Article A Reinforcement Learning Based Framework for Prediction of Near Likely Nodes in Data-Centric Mobile Wireless Networks" pdf
Ngày tải lên :
21/06/2014, 18:20
... curve of the trace -based scheme lies to the left side of the point -based scheme This shows that in terms of neighborhood prediction accuracy, the trace -based scheme outperforms the pointbased ... (×100%) 0.8 Trace based, average number of Neighbors 15 Point based, average number of Neighbors 15 Trace based, average number of Neighbors 75 Point based, average number of Neighbors 75 (b) ... CDF of the difference of the future probability Prdt to the past probability Prdt of the near likely node Figure presents the CDF of the probability difference for both pointbased and trace-based...
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Báo cáo hóa học: " Research Article A Nonlinear Decision-Based Algorithm for Removal of Strip Lines, Drop Lines, Blotches, Band Missing and Impulses in Images and Videos" docx
Ngày tải lên :
22/06/2014, 00:20
... Additionally, impulse noise is a standard type of degradation in remotely sensed images This paper considers application of median -based algorithms for removal of impulses, strip lines, drop lines, band ... better solution for removal of impulse noise with better edge and fine detail preservation Several adaptive algorithms [6–9] are available for removal of impulse noises However, none of these algorithms ... log10 √ (10) The performance of several new algorithms [12–14] in respect of impulse noise removal is shown in Table The proposed algorithm also performs well in removal of impulse noise along...
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Báo cáo hóa học: "Research Article Colour Vision Model-Based Approach for Segmentation of Traffic Signs" doc
Ngày tải lên :
22/06/2014, 06:20
... during recognition stage Based on this information, image classification can be carried out based on the saturation of sky or the texture of the road The degree of saturation of the sky (blue colour ... have developed several additional procedures based on the estimation of shape, size, and location of primarily segmented areas to improve the performance of RGB method Miura et al [15] use both colour ... group of researchers in UK between middle 1980s and early 1990s running two 3year projects consecutively They based on Hunt’s colour vision model [20–23] of a simplified theory of colour vision for...
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Báo cáo y học: "ExpressionPlot: a web-based framework for analysis of RNA-Seq and microarray gene expression data" doc
Ngày tải lên :
09/08/2014, 23:20
... to the different isoforms The relative strength of the segments corresponds to the abundances of the two isoforms (Figure S9 in Additional file 1) Examining levels or changes of particular genes ... brad.aaron.friedman@gmail.com Department of Molecular and Cell Biology, Harvard University, Divinity Ave, Cambridge, MA 02138, USA Full list of author information is available at the end of the article ExpressionPlot ... not adjusted for multiple testing This should be kept in mind when setting cutoffs on the website We usually use a P-value cutoff of 10 For example, using the UCSC genes cluster for mouse (mm9)...
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Báo cáo sinh học: "HuMiTar: A sequence-based method for prediction of human microRNA targets" ppt
Ngày tải lên :
12/08/2014, 17:20
... of the MuMiTar algorithm Pseudo-code of the MuMiTar algorithm Page 11 of 12 (page number not for citation purposes) Algorithms for Molecular Biology 2008, 3:16 uation of the results, and performed ... Comparison of the sensitivity, number of predicted targets and overlap between the predictions of different methods Page of 12 (page number not for citation purposes) Algorithms for Molecular Biology ... imperfect pairing and inclusion of information based on the stacked pairs and unpaired regions Page 10 of 12 (page number not for citation purposes) Algorithms for Molecular Biology 2008, 3:16 Methods...
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Báo cáo y học: "Gene expression-based screening for inhibitors of PDGFR signaling" potx
Ngày tải lên :
14/08/2014, 08:20
... were selected for each one of three pairs of conditions: cells with PDGF versus cells without PDGF, cells with PDGF versus cells with PDGF and apigenin, and cells with PDGF versus cells with PDGF ... in the design of experiments, interpretation of data, writing of the manuscript, or planning of its submission for publication References Johnston P: Cellular assays in HTS Genome Biology 2008, ... different from the extracellular receptor inhibition mechanism of ATA Discussion In this report, we describe the proof -of- concept efforts to approach the discovery of inhibitors of signal transduction...
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A behaviour based algorithm for encirclement of a dynamic target using multiple mobile robots
Ngày tải lên :
15/09/2015, 22:43
... addition, we will study the performance of this algorithm for different number of robots used and for different speed ratio (target speed / robot speed) Part of the results of this project has been ... Index 28 4.5.2 Effects of Parameters on Performance Index 29 4.6 4.7 General Law for Performance Index of Encirclement .34 Chapter Summary 36 VALIDATION OF ENCIRCLEMENT ALGORITHM ... 2.1 2.2 Behaviour -Based Control of Multiple Robots .7 2.3 Circle Formation of Distributed Mobile Robots Chapter Summary DESIGN OF ROBOTIC BEHAVIOURS FOR ENCIRCLEMENT 10...
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Protective effects of hydrogen sulfide against 6 hydroxydopamine induced cell injury implication for treatment of parkinsons disease
Ngày tải lên :
13/10/2015, 15:57
... 42 2.2.4 Cell viability assay 42 2.2.5 Cell fractionation for determining PKC isoform translocation 43 2.2.6 Preparation of cell lysates for the detection of TH and phosphorylated ... SH-SY5Y cells 50 2.3.4 Effect of NaHS on cell viability in 6-OHDA-treated SH-SY5Y cells in the presence and absence of inhibitors of PKC isoforms 50 2.3.5 H S-induced neuroprotection ... …47 Figure 2.3 Role of PKC isoforms in the neuroprotective effects of NaHS in SHSY5Y cells treated with 6-OHDA………………………………………………… 49 Figure 2.4 The protective effect of NaHS on cell viability in...
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Báo cáo sinh học alignminer a web based tool for detection of divergent regions in multiple sequence alignments of c
Ngày tải lên :
20/12/2015, 08:16
... Algorithms for Molecular Biology 2010, 5:24 http://www.almob.org/content/5/1/24 Page 14 of 16 Figure Analysis of two Pinus pinaster gene isoforms The specific primer pair for the photosynthetic isoform ... thaliana AtGS1 isoform AY088312 Arabidopsis thaliana Arabidopsis thaliana AtGS1 isoform AtGS1 isoform AY059932 AK118005 AC# (amino acid) Q56WN1 70% Q9LVI8 Plant Oryza sativa OsGS1 isoform AB037664 ... identified by a “P” and for the non-photosynthetic isoform by an “N” (A) Predicted sequence and properties of the two primer pairs designed for specific identification of each isoform (B) PCR analysis...
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Báo cáo khoa học: New roles of flavoproteins in molecular cell biology: An unexpected role for quinone reductases as regulators of proteasomal degradation pptx
Ngày tải lên :
16/03/2014, 02:20
... recognition of p53 involves post-translational modifications Accumulation of p53 then results in the expression of a variety of genes necessary to cope with DNA damage and other forms of cellular ... (the 66 kDa isoform of ShcA, an adaptor protein that relays extracellular signals downstream of receptor tyrosine kinases) The latter protein gives rise to increased levels of intracellular ROS, ... of damaged cells [46,47] This generation of ROS increases expression of NQO1 [48] which then further stabilizes p53 This sequence of events is consistent with the proposed feed-forward loop for...
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Báo cáo khoa học: Systems biology: model based evaluation and comparison of potential explanations for given biological data pot
Ngày tải lên :
23/03/2014, 06:20
... explanations, based on information that is not immediately apparent from the data For example, in the evaluation of the importance of re-cycling of STAT5 [9–11], a primary argument for the importance of ... rejected based on a lack of agreement with the available data alone After that, we review methods for comparison of the predictive ability of two models, and finally suggest a scheme for the general ... Model based evaluation in systems biology as a way of controlling the flexibility of the model Certain regularization methods [15,16] can also be used for regressor selection The main idea of regularization...
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Báo cáo y học: " Major biological obstacles for persistent cell-based regeneration of articular cartilage" pps
Ngày tải lên :
09/08/2014, 10:20
... approaches, the reasons for the failure of cellbased cartilage repair approaches to form hyaline repair tissue in vivo remain largely unclear to date [2,27-29] Although proof of principle has been ... stem cells and cell- based tissue engineering Arthritis Res Ther 2003, 5:32-45 Giannoni P, Cancedda R: Articular chondrocyte culturing for cell- based cartilage repair: needs and perspectives Cells ... Mesenchymal cell- based repair of large, fullthickness defects of articular cartilage J Bone Joint Surg Am 1994, 76:579-592 Trippel SB, Ghivizzani SC, Nixon AJ: Gene -based approaches for the repair of...
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Báo cáo y học: "Comparative study between the Hybrid Capture II test and PCR based assay for the detection of human papillomavirus DNA in oral submucous fibrosis and oral squamous cell carcinoma" pdf
Ngày tải lên :
12/08/2014, 01:22
... sample Interpretation of Cut off value (RLU of specimen/mean RLU of PC) Cut-off ratio of to 0.99 is negative for HR HPV; Cutoff ratio greater than 1.0 positive for HR HPV The cut off ratio at 1.0 ... intensity of HPV infection This ratio of any specimen represents empirically a relative measure of the viral load in it Cut off value (RLU of specimen/ mean RLU of PC) was 1.0 pg/ml of sample ... of HPV viral load Ratio of relative light unit (RLU) of the sample/mean of RLU of three positive controls (PC) were taken as an estimate of approximate viral load which relates to the index of...
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báo cáo khoa học: " A simple and high-sensitivity method for analysis of ubiquitination and polyubiquitination based on wheat cell-free protein synthesis" potx
Ngày tải lên :
12/08/2014, 03:20
... regulator Identification of activity and features of E3 ligases offers important information about the ubiquitin-dependent regulation system Our luminescent method based on the wheat cell- free system ... http://www.biomedcentral.com/1471-2229/9/39 Effect of5 Figure proteasome inhibitor on stability of polyubiquitinated proteins Effect of proteasome inhibitor on stability of polyubiquitinated proteins Polyubiquitination assays of crude ... of target proteins [6], and Lys-63 linked chains reg- Page of 11 (page number not for citation purposes) BMC Plant Biology 2009, 9:39 ulate signal transduction such as cellular localization of...
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Báo cáo sinh học: "Immediate transfection of patient-derived leukemia: a novel source for generating cell-based vaccines" ppt
Ngày tải lên :
14/08/2014, 19:22
... Upon comparison of RFP expression levels at day 1, the superiority of electroporation -based transfection was evident In the viable fraction of nucleofected cells, 60% of these cells expressed ... to 15% of the novafected cells Even when corrected for cells lost to electroporation-associated cell death, the nucleofection expression rate is 26% As a control, cells were also nucleofected ... number of U2OS, SCCVII and AGN2a tumor cells postnucleofection from separate experiments, expressed as a percentage of the number of cells originally nucleofected cytometry in the nucleofected...
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Development of a MACS based strategy for isolating rare cell populations from animal tissue for transcription factor studies
Ngày tải lên :
11/09/2015, 09:59
... transcription profile of that particular cell type in the normal state, rather than the profile of the entire organ Having samples consisting purely of cells of interest is especially relevant for developmentally ... epithelia of the Peyer‟s patches of the gut epithelial lining Being responsible for the transport of foreign material across the Page 19 barrier for immune system recognition, microfold cells are of ... new cell isolation strategies upon The cell isolation strategy we developed defines the cells of interest by the specific expression of one or two genes of interest Here, we define our cells of...
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Cell based screening assay for inhibitors of porcine circovirus type2 (PCV2) replication
Ngày tải lên :
03/10/2015, 11:36
... 1.3.1 Cell based and Cell free Assays Generally, assays in drug discovery are classified as either cell based or target based techniques. In the former, whole cell phenotypic ... is necessary to optimize these cell based screening assays for inhibitors of PCV2 replication. Cell Based Screening Assay for Inhibitors of Porcine Circovirus Type 2 (PCV2) Replication ... (GraphPad Software, San Diego California USA, www.graphpad.com). One‐way Analysis of Variance (ANOVA) with Bonferroni’s post‐test was performed for data obtained for Cell ELISA. Cell Based Screening Assay for Inhibitors of Porcine Circovirus Type 2 (PCV2) Replication ...
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