cd8 t cell immune responses in the splenocytes of tumor bearing mice
Báo cáo sinh học: " A combined nucleocapsid vaccine induces vigorous SARS-CD8+ T-cell immune responses" potx
... protein synthesised sufficient protein within infected cells to facilitate the formation of VLPs Detection of antibody titer in mice immunized with the candidate vaccine combinations In order to analyze ... neither the mock-transfected cells nor the cells transfected with the vector alone showed viral-like particles (Fig 2) These observations demonstrate that our construct expressing the NC protein ... (TLR9), inducing cell maturation and enhancing antigen presentation and Th1 responses [45-47] The combination of montanide and CpG was investigated in light of a recent study demonstrating that this...
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Báo cáo y học: "The role played by cell-substrate interactions in the pathogenesis of osteoclast-mediated peri-implant osteolysis" potx
... induction of the cathepsin K and TRAP genes Importantly, the transcriptional activation of the β3 integrin and CTR genes coincides with the transition of the osteoclast to an actively resorbing cell ... expressing TRAP activity as well as CTR Most importantly, these cells were able to resorb the bone matrix, thus establishing their authenticity as osteoclast cells These observations, and the findings ... that neither the β3 integrin nor the CTR are expressed by osteoclast precursors The expression of these genes increases during the late stages of osteoclast differentiation [42], after induction...
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Báo cáo y học: " Immune mechanisms in the pathogenesis of idiopathic inflammatory myopathies" doc
... IgG1 antibody (Becton Dickinson) in a consecutive section to that in (b) (d) H&E staining to localize inflammatory cell infiltrates in a patient with dermatomyositis (e) CD4+ T lymphocytes stained ... speculated that they might interact with antigens (from viruses or other sources) expressed in various pulmonary cells (Figure 3) It is interesting in this context that there is a reported tissue ... switching or the antigen-binding properties of the antibody, respectively, resulting in the production of antibodies of various isotypes that can be distributed to various body compartments B...
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Báo cáo y học: "Reduced proportions of natural killer T cells are present in the relatives of lupus patients and are associated with autoimmunity" ppt
... proportion of NKT cells is a heritable trait These findings raise the possibility that one of the explanations for the clustering of multiple autoimmune disorders within the families of lupus patients ... Excellence of the University of Toronto and is the recipient of The Arthritis Society/CIHR Investigator Award Dr Fortin is funded by an Investigator Award from The Arthritis Society/CIHR Institute ... the proportion of plasma cells within the PBMC population was significantly reduced after overnight transport Because the majority of these cells are not contained within the lymphoid gate, the...
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Báo cáo y học: "Roles of planar cell polarity pathways in the development of neutral tube defects" ppsx
... sensitive to the orientation of the cell s sides with respect to the global axis of the epithelium, allows the polarity of the cell to be established within the plane of the epithelium and promotes ... data and information collection and part of the writing XH performed part of text writing and the editing of the whole manuscript YH wrote the part of the manuscript and information collection ... in anatomy, tissue types and morphogenetic processes, together with the existence of a number of distinct PCP components make it interesting to think about the difference in the development of...
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Báo cáo y học: " Immune modulation in the treatment of respiratory infection" potx
... 10 patients with severe ventilator-dependent pneumonia, at baseline and after the initiation of G-CSF treatment as an adjunct to standard therapy These investigators showed that treatment with ... and the effect on the time to resolution of morbidity [25] (TRM), which was a clinical index of factors associated with the need for continued treatment in hospital The TRM was days in both groups ... paradigm shift in recent years from inhibiting pro-inflammatory cytokines to selectively augmenting the cytokine response within a specific compartment in infected patients that have some objective parameter...
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Differential host immune responses in BALB c and C57BL 6 mice to burkholderia pseudomallei infection
... anti-inflammatory cytokine (IL-10) compared to the splenocytes of C57BL/6 mice This study suggests that resistance to infection lies in the innate ability to control the bacterial growth that ... of C57BL/6 to the infection Studies using animal models suggest that the course of infection is highly dependent on the infective dose, the route of infection and the virulence of the infecting ... suppurative infection to potentially fatal septicemia The aim of this project is to study the host responses and factors which contribute to resistance or susceptibility in two strains of mice showing...
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Role of c jun in the regulation of tumor suppressor p53 homologue, p73
... co-exist in tumors This study shows for the first time that p73 increases AP-1 (5XTRE) activity and it synergies with c-Jun to potentiate AP-1 activity The transactivation domain (TAD1) near the ... significant ability to bind and interact with TAp73-α In co-transfection assays, it has been shown that the 175, 248 and 281 mutants of p53 interact with p73-α and inhibits its ability to transactivate ... suggest that p73 is also activated at the transcriptional level in response to a DNA damaging drug, Campothesin (Chen et al., 2001) In addition, information is lacking about the interactions of the...
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Báo cáo y học: " The intracellular detection of MIP-1beta enhances the capacity to detect IFN-gamma mediated HIV-1-specific CD8 T-cell responses in a flow cytometric setting pro" ppsx
... modality of evaluation equivalent to the measurement of the total IFN-γ producing T- cells with the relevant advantage of a consistent decrease of the background that in turn increases the sensitivity ... represent an interesting option to increase the sensitivity of the ICS assay in the detection of IFN-γ mediated HIV-1-specific responses In order to compare the sensitivity of the two modalities to evaluate ... rare Since the analysis of the combination of two functions decreases the background and the measurement of the IFN-γ+ MIP-1β+ T- cells was equivalent to the measurement of the total IFN-γ+ T- cells,...
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The role of interferon gamma in regulating antigen specific CD8 t cell responses in a mouse model of influenza
... glycoproteins on the surface of the virus HA is a lectin that mediates the binding of the virus to target cells and facilitates the entry of the virus into the target cell The proteolytic cleavage of ... encoding the matrix protein The M2 protein comprises of the ectodomain M2e, a small part exposed to the cell surface, a transmembrane domain with the rest of it localized within the internal portion ... matter of hours, whereas the adaptive immunity takes days to be initiated and then for the response to peak In the interim, it is the innate immune response that combats viral replication in the initial...
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Báo cáo hóa học: " Impairment of the CD8+ T cell response in lungs following infection with human respiratory syncytial virus is specific to the anatomical site rather than the virus, antigen, or route of infection" pdf
... Thus, it is not the virus bearing the epitope nor local virus replication that results in the decreased functionality of CD8+ CTL in lungs, but rather the pulmonary site of residence of the cells ... of tetramer +CD8+ cells In contrast, the number of splenic IFNγ +CD8+ cells was 89% that of the tetramer +CD8+ cells Importantly, the virus-specific CD8+ cells isolated from the lungs of mice infected ... (15%) of tetramer +CD8+ cells was detected in the lungs after IN infection with VV-M2 Interestingly, despite the lack of VV-M2 replication in the lungs after ID inoculation, a high level (21%) of tetramer +CD8+ ...
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Báo cáo y học: "trong mucosal immune responses in SIV infected macaques contribute to viral control and preserved CD4+ T-cell levels in blood and mucosal tissues" pps
... proportions of CD4+CCR5+ T- cells in both gut sites of controllers argues for a repopulation of mainly CD4+CCR5- T- cells The reduction of the primary viral target cells in the intestine, the largest ... However, the cytokine secretion in their studies was related to the total amount of CD4+ or CD8+ T- cells and the different ratio between naïve and memory T- cells in blood and gut was not considered In ... designed and coordinated the study and edited the manuscript All authors read and approved the final manuscript Competing interests The authors declare that they have no competing interests Received:...
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Báo cáo y học: "Escape is a more common mechanism than avidity reduction for evasion of CD8+ T cell responses in primary human" pdf
... mutation had been transmitted that then reverted, stimulating expansion of T cells able to recognise the original epitope with higher avidity than the initial response [35] In the other epitopes, ... positive Measurement of the functional avidity of CD8+ T cell responses The functional avidity of CD8+ T cell responses at sequential time-points during the first year of HIV infection was determined ... sequence of the epitope at the indicated timepoint (day (d) FOSx is shown Areas of amino acid variation within the epitope are indicated in bold italics and underlined the virus population within the...
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Báo cáo y học: "Viral suppression of multiple escape mutants by de novo CD8+ T cell responses in a human immunodeficiency virus-1 Infected elite suppressor" ppt
... no mutations in either the IW9 or TW10 epitopes, suggesting that mutations in the HLA-B*57 restricted epitopes are not the only factors impacting the fitness of virus from ES8 To elucidate the ... them from the dominant proviral variants These plasma Gag clones were identical to each other with the exception of variation at the TW10 epitope In total, seven different variations in the TW10 ... variants Representative dot plot and histogram shown for stimulation with one of the autologous mutants, TSTLTEQVAW (top left) and wild type TW10 (top right) Gating is on CD8+ T cells (bottom) Total...
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Báo cáo y học: "Adoptive transfer of splenocytes to study cell-mediated immune responses in hepatitis C infection using HCV transgenic mice" pptx
... proliferate, the cell population shifts to a lower intensity due to the decrease of staining in the cell membranes of proliferating cells Daughter cells have half the fluorescent intensity of the ... in the liver of the transgenic mice but not in mouse spleen or lymph nodes In this study we did not detect the fate of the transferred cells; nonetheless, it seems that these cells have the potential ... and the Y axis indicate the percentage of the CFSE+ CD4+ or CD8+ T cells B) The percentage of donor CD4+ and CD8+ T cells in the blood seven days after the injection The cells were surface stained...
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The role of CD8 t cells in the differentiation of TNF iNOS producing dendritic cells and TH1 responses
... (Gurunathan et al., 2000), suggesting that CD8 T cells were vital for the polarization of Th1 cells This was also supported by studies demonstrating the involvement of CD8 T cells in the generation ... helper-dependent cytotoxic responses In particular, pre-stimulation of DCs with anti-CD40 in vitro, or the injection of anti-CD40 antibodies into helper T- cell deficient mice, restored the ability of DCs to ... Adaptive immunity Thought to have arisen in the first jawed vertebrates, the adaptive or "specific" immune system is initiated by cells of the innate immune system, such as DCs The adaptive immune...
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Báo cáo khoa học: Intrabodies against the EVH1 domain of Wiskott–Aldrich syndrome protein inhibit T cell receptor signaling in transgenic mice T cells potx
... 5¢-CACCCAAGCTT GCCACCATGGAGGTTCAGCTGCAGCAGTCTG-3¢; primer 7, 5¢-GGT GGAGGAGGTTCTGATGTTTTGATGACCCAAACTCCAC-3¢; primer 8, 5¢-CGAATGCGGCCGCCCGTTTGATTTCCAGCTTGGTGC-3¢; primer 9, 5¢-GGTGGAGGAGGTTCTGATGTTGTTCTGACCCAAACTCCACTC-3¢; ... bind to the target 6132 proteins on the lumen side and inhibit transport of target proteins in the process of functional maturation [6,8] If the targets are cytosolic proteins, scFvs without the ... noncoding linker containing an EcoRI site at the 5¢ end of the linker (5¢-AATTCTACAGG TCCTCCTCGCTGATCAGCTTCTGCTCCGAACCTGC-3¢) were annealed and inserted into the NotI ⁄ EcoRI site of all pCAG ⁄ anti-WASP...
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báo cáo hóa học:" Protective CD8+ T-cell responses to cytomegalovirus driven by rAAV/GFP/IE1 loading of dendritic cells" pdf
... shown in Figures 4, 5, to be insignificant targets http://www.translational-medicine.com/content/6/1/56 Competing interests The authors declare that they have no competing interests Authors' contributions ... loading and a zero virus control (PBMC only) The cytotoxicity of the stimulated T- cells directly correlated with the amount of AAV/IE1 used to load the Figure Cytotoxicity assay Cytotoxicity assay ... loading DCs at day Alternately, the addition of anti-class I antibodies significantly inhibited the killing activity (P < 0.05), suggesting that CTLs were MHC class I restricted The CTL stimulation...
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Báo cáo hóa học: " Fowlpox virus recombinants expressing HPV-16 E6 and E7 oncogenes for the therapy of cervical carcinoma elicit humoral and cell-mediated responses in rabbits" pot
... represent the main target for immune therapy, as they maintain the proliferative state and prevent apoptosis [49,50] In the present study, we have described the complete humoral and cellular immune responses ... fold increase 12 Rabbit * T1 T2 T3 T4 T5 P1 P2 bleeding times P3 P4 T1 T2 T3 T4 T5 P1 P2 bleeding times P3 P4 Figure Th1/Th2 cytokine determination by the QuantiGene 2.0 Reagent system The RNAs of ... improvements of the recombinants, using the E6 and E7 transgenes deleted of the p53 and p105Rb cellular binding domain, might further increase the safety of the vaccine Recently, a p53 degradation-defective...
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Báo cáo hóa học: "Programmed cell death-1 (PD-1) at the heart of heterologous prime-boost vaccines and regulation of CD8+ T cell immunity" doc
... priming, the epitopespecific CD8+ T cells, although few in numbers (~1/100 specific/total CD8+ T cells), had some strikingly distinguishing features Within the population of CD8+ T cells initiated ... under the rat insulin promoter The PD-1 -/- T cells proliferated to a higher extent in draining lymph nodes and caused insulitis and diabetes, in dramatic contrast to wild-type PD-1competent T cells ... observed with other vectors in a distinct model, clearly supporting a precise sequence of administration of vectors as a major factor determining the magnitude of immunity [40], although this hypothesis...
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