... 22 23 24 25 26 Multilineage potential of adult humanmesenchymalstemcells Science 1999, 284:143-147 Prockop DJ: Marrow stromal cells as stemcells for nonhematopoietic tissues Science 1997, ... A, Sekiya I: Increased proliferation of human synovial mesenchymalstemcells with autologous human serum: comparisons with bone marrow mesenchymalstemcells and with fetal bovine serum Arthritis ... After to days, the medium was changed to remove nonadherent cells, and the adherentcells were cultured for days as passage without refeeding The cells were then trypsinized, harvested and resuspended...
... stemcells from whole human umbilical cord tissue J Cellular Biochem 2009, 107:926-932 Yang C-C, Shih Y-H, Ko M-H, Hsu S-Y, Cheng H, Fu Y-S: Transplantation of Human Umbilical MesenchymalStemCells ... Review: Human Umbilical Cord Stroma with Regard to the Source of Fetus-Derived StemCellsStemcells 2007, 25:2886-2895 Wu KH, Mo XM, Zhou B, Lu SH, Yang SG, Liu YL, Han ZC: Cardiac potential of stem ... Pluripotency in Human Embryonic StemCellsStemCells Dev 2008, 17:1195-1206 Rodgers A, Mormeneo D, Long JS, Delgado A, Pyne NJ, Pyne S: Sphingosine 1-Phosphate Regulation of Extracellular Signal-Regulated...
... Stemcells 1.5.1.1 Embryonic stemcells 1.5.1.2 ES cellstem therapy is not ideal for clinical use 1.5.2 Adult stemcells 10 1.5.2.1 Bone marrow stemcells ... research 1.5.2.1 Bone marrow stemcells There are most common types of stemcells in bone marrow: (i) hematopoietic stemcells and (ii) mesenchymalstemcells These cell types are believed to be ... has since been believed that hematopoietic stemcells may not differentiate into cardiomyocytes45 1.5.2.1.2 MesenchymalstemcellsMesenchymalstemcells (MSCs) encompass a heterogeneous population...
... annulus cells adiposederived mesenchymalstem of AD-MSCfor 3D co cultured alone Increase in separate culturecells (AD-MSC) and annulus cells comderived mesenchymalstemcells (AD-MSC) and annulus cells ... the effect of stem cells, or stem cell- conditioned media (CM), on disc cells Previous experiments with disc cells have shown that co -culture of nucleus pulposus with annulus fibrosis cells stimulated ... disc cells in the 3D culture after two weeks When disc cells were cultured alone, the CFSE label was clearly visible in almost all cells Both labelled and unlabelled cells were visible in co-cultures...
... from human placenta StemCells 22, 1338–1345 Bieback K, Kern S, Kluter H & Eichler H (2004) Critical parameters for the isolation of mesenchymalstemcells from umbilical cord blood StemCells ... Epithelial mesenchymal transitions: twist in development and metastasis Cell 118, 277–279 31 Zipori D (2004) Mesenchymalstem cells: harnessing cell plasticity to tissue and organ repair Blood Cells ... hepatic cells, the findings presented here suggest that MET might be a pivotal factor in determining stemcell transdifferentiation Discussion AT-MSCs may be good candidates as stemcells for cell...
... Morphology of adherentcells when isolated from hFTs (primary cultures) A): Cells cultured for three days after initial plating Cells with an MSC-like phenotype and a small cluster of cells with ... Multipotent stemcells from umbilical cord: cord is richer than blood! StemCells 2008, 26(1):146-150 Zhang MJ, Liu B, Xia W, Sun ZY, Lu KH: Could cells from menstrual blood be a new source for cell- based ... T, Sakamoto M, Ogawa S, Umezawa A: Novel cardiac precursor-like cells from human menstrual blood-derived mesenchymalcellsStemCells 2008, 26:1695-1704 Nasef A, Zhang YZ, Mazurier C, Bouchet...
... ‘bone marrow stemcell , which is often used for either hematopoietic or mesenchymalstem cells, the central nervous system stem cell, the hepatic stem cell, etc.? If many stemcells are found ... and regeneration [134] Mesenchymalstemcells versus embryonic stemcells Embryonic stem (ES) cells are derived from the inner cell mass of the embryonic blastocyst These cells can be maintained ... three main cell types: endothelial cells, hematopoietic stem cells, and stromal cells In a ground-breaking study, Friedenstein et al [30] isolated cells, clonogenic fibroblast precursor cells (CFU-F),...
... of cells was determined spectrophotometrically entiation of humanmesenchymal transcripts Expression of BMP-2 and chordinstem cells during osteogenic differentiation of humanmesenchymalstemcells ... between 100 and 1,000 cells/ cm2, trypsinised and stored in Recovery CellCulture Freezing Medium in liquid nitrogen Before osteogenic differentiation, 6,000 cells/ cm2 of human MSCs were seeded ... of MSCs MSCs were cultured in BM or OM for 20 days The usual change in phenotype of MSCs from spindle-shaped cells (when cultured in BM) to more cuboidal-shaped cells (when cultured in OM) was...
... of cultured humanmesenchymalstemcells from marrow Tissue Eng 1998, 4:415-428 35 Mueller MB, Tuan RS: Functional characterization of hypertrophy in chondrogenesis of humanmesenchymalstemcells ... label viable cells This combination allows the differentiation among early apoptotic cells (Ann5 positive, 6-CFDA positive), necrotic cells (Ann5 positive, 6-CFDA negative), and viable cells (Ann5 ... with humanmesenchymalstemcells Tissue Eng 2002, 8:131-144 24 Johnstone B, Hering TM, Caplan AI, Goldberg VM, Yoo JU: In vitro chondrogenesis of bone marrow-derived mesenchymal progenitor cells...
... size-sieved stemcells into electrically active neural cellsStemCells 2002, 20(6):522-529 Tzeng SF, Tsai MJ, Hung SC, Cheng H: Neuronal morphological change of size-sieved stemcells induced ... of humanmesenchymalstemcells into insulin producing cells J Biomed Mater Res A 2008, 86(4):1097-1105 Chao KC, Chao KF, Fu YS, Liu SH: Islet-like clusters derived from mesenchymalstemcells ... cells on supporting endogenous cells to regenerate However, the potential of bone marrow stemcells or MSCs to differentiate into IPCs has been demonstrated in vitro and in vivo Bone marrow cells...
... of stemness and stemcell niche of mesenchymalstem cells: implications in tumorigenesis and metastasis J Cell Physiol 2010, 222:268-277 Ohishi M, Schipani E: Bone marrow mesenchymalstemcells ... from mutated human embryonic bone marrow mesenchymalstemcells Oncol Rep 2004, 12:501-508 22 Lee HJ, Choi BH, Min BH, Park SR: Changes in surface markers of humanmesenchymalstemcells during ... staining (Figure 1B) of HPBAML-I cells revealed that this cell line is composed of round-polygonal and spindle-like cells Unlike the round-polygonal cells, HPB-AML-I cells with the spindle-like morphology...
... medium, and the cells were seeded in 10 cm culture dishes After 24 hours, non adherent (hematopoietic) cells were removed by medium change Mesenchymalstemcells were expanded in stemcellculture medium ... (about 10,000 cells/ cm2), stemcells were trypsinized with stemcell trypsin (CellSystems, St Katharinen, Germany) and re-plated at a density of 1000/cm2 to expand cellculture (cell proliferation ... properties, suppressing the activity of a broad range of immune cells, including N -cells, Bcells, natural killer cells and antigen presenting cells Transplantation of MSCs into the periinfarct area decreases...
... activity of human bone marrow mesenchymalstemcellsStemCells 2006, 24:386-398 Meisel R, Zibert A, Laryea M, Gobel U, Daubener W, Dilloo D: Human bone marrow stromal cells inhibit allogeneic T -cell ... Figure Mesenchymalstemcells (MSCs) inhibit the proliferation of CD4 + T cells in vitro by induction of nitric oxide and prostaglandin E2 (PGE2) (a) CD4+ T cells, in the presence of accessory cells, ... 11 Mesenchymalstemcell treatment has no effect on the development of collagen-induced arthritis Figure Mesenchymalstemcells (MSCs) inhibit the anti-CD3induced proliferation of CD4+ T cells...
... Neyrinck AP, Matthay MA, Lee JW: Allogeneic humanmesenchymalstemcells restore epithelial protein permeability in cultured human alveolar type II cells by secretion of angiopoietin-1 J Biol ... undifferentiated mesenchymalstemcells Exp Hematol 2003, 31:890-896 Tse WT, Pendleton JD, Beyer WM, Egalka MC, Guinan EC: Suppression of allogeneic T -cell proliferation by human marrow stromal cells: ... Lee JW, Matthay MA: Antibacterial effect of humanmesenchymalstemcells is mediated in part from secretion of the antimicrobial peptide LL-37 StemCells 2010, 29:2229-2238 11 Rocha V, Wagner...
... canine adipose tissue derived mesenchymalstemcells In order to demonstrate that the cells isolated from the canine adipose tissue (Figure 1A) are indeed mesenchymalstemcells (MSCs) we differentiated ... concentration of 500 μM and stored at -80°C Serial dilutions were prepared in culture medium CellcultureMesenchymalstemcells (MSCs) were isolated from canine adipose tissue biopsies and primary ... of human bone marrow mesenchymal progenitor cells J Cell Physiol 1999, 181:67-73 20 Jaiswal N, Haynesworth SE, Caplan AI, Bruder SP: Osteogenic differentiation of purified, culture- expanded human...
... disc cell- mediated mesenchymalstemcell differentiation StemCells 2006, 24:707-716 Le Visage C, Kim SW, Tateno K, Sieber AN, Kostuik JP, Leong KW: Interaction of humanmesenchymalstemcells ... F, Mochida J: Human nucleus pulposus cells significantly enhanced biological properties in a coculture system with direct cell- to -cell contact with autologous mesenchymalstemcells J Orthop ... disc-like cells from adult mesenchymalstemcellsStemCells 2005, 23:403-411 11 Yoshikawa T, Ueda Y, Miyazaki K, Koizumi M, Takakura Y: Disc regeneration therapy using marrow mesenchymalcell transplantation:...
... of amniotic fluid-derived humanmesenchymalstemcellsStemCells Dev 2006, 15:719-726 55 Montaln H, Schichor C, Lah TT: Humanmesenchymalstemcells and their use in cell- based terapies Cancer ... Jorgensen B, Feldmann S, Ehringer G, Bornhauser M, Werner C: Mesenchymalstemcells can be differentiated into endothelial cells in vitro StemCells 2004, 22:377-384 47 Chelucci C, Federico M, Guerriero ... direct staining of cell cultures with red oil The microscopic evaluation of the red oil stained cell cultures showed a reliable increase in red oil stained cells in the cell cultures treated with...