apoptosis and dna damage in human spermatozoa

Báo cáo khoa học: Apoptosis and autophagy: Regulation of apoptosis by DNA damage signalling – roles of p53, p73 and HIPK2 ppt

Ngày tải lên : 16/03/2014, 00:20

... the cell nucleus and the key molecules p53, p73 and homeodomain-interacting protein kinase (HIPK2) Multiple functions of p53 in DNA damageinduced apoptosis The tumour suppressor and transcription ... an increased SUMOylation and stability of PML, suggesting an additional role of HIPK2 in regulating DNA damageinduced cell death Collectively, these ﬁndings indicate that HIPK2 is involved in DNA ... p73 and HIPK2 in apoptosis N Bitomsky & T G Hofmann Fig Regulation of DNA damageinduced cell death by p53 and HIPK2 Genotoxic stress-induced DNA damage facilitates activation of the DNA damage- activated...

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Tài liệu Báo cáo khoa học: The small heat shock proteins and their role in human disease pptx

Ngày tải lên : 19/02/2014, 18:20

... formation of huntingtin-containing inclusion bodies in Huntington’s disease encourages cell survival, whereas monomers and small inclusion bodies of huntingtin, a protein possessing abnormal polyQ ... aB-Crystallin R120 corresponds to aA-crystallin R116 and both are conserved a-crystallin domain arginines R120G aB-crystallin permits intermediate ﬁlament self association in vitro, although binding ... aAcrystallin requires this domain [66] Attachment of R116C aA-crystallin to Hsp27 and aB-crystallin increases in comparison to wild type, while binding to cC-crystallin and bB2-crystallin decreases...

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Department of Reproductive Health and Research including the UNDP/UNFPA/WHO/World Bank Special Programme of Research, Development and Research Training in Human Reproduction (HRP) doc

Ngày tải lên : 23/03/2014, 06:20

... worked in reducing maternal mortality include those relating to innovative financing measures; investment in human resources, both in terms of strengthening pre-service education and emphasizing in- service ... NGOs and other institutions Improving maternal and perinatal health working in the field on maternal and infant health Work with Doctors with Africa CUAMM, an NGO operating in Africa since 1955 in ... Hypertension in Pregnancy (ISSHP), in Geneva WHO will be leading and coordinating a number of sessions in both Congresses increasing visibility and repositioning HRP's work and priorities in the global...

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Báo cáo khoa học: Activation of p21-activated kinase 1 is required for lysophosphatidic acid-induced focal adhesion kinase phosphorylation and cell motility in human melanoma A2058 cells potx

Ngày tải lên : 30/03/2014, 13:20

... turn recruits other proteins, including paxillin, p130Cas, vinculin and talin in focal adhesions These protein complexes anchor the actin cytoskeleton and provide structural integrity to cells [16] ... either tyrosine kinase inhibitor, genistein, or MAP kinase inhibitor, PD98059 Inhibition of tyrosine kinase by genistein greatly inhibited LPA-induced PAK activation, whereas MAP kinase activity ... endothelial cells [52] and G protein coupled receptor kinase (GRK)-interacting targets (GIT) links PAK and FAK [53], involvement of PIX, Nck and GIT in LPA-induced FAK phosphorylation and cell motility...

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Báo cáo hóa học: " Role of protease-activated receptor-2 on cell death and DNA fragmentation in Helicobacter pylori-infected gastric epithelial cells" ppt

Ngày tải lên : 18/06/2014, 16:20

... MAPK In contrast, inhibition of p38 by a specific inhibitor SB203580 decreased apoptosis while ERK1/2 inhibition by a specific inhibitor PD98059 resulted in an increase of apoptosis in H pylori-infected ... expression using PAR-2 AS ODN in AGS cells Additionally, inhibition of PAR-2 activity using trypsin inhibitor increased cell death and DNA fragmentation in H pylori-infected AGS cells These results ... activation of multiple signaling pathways, including MAPK cascades in distinct cell types [16,17] PAR-2 is involved in cell proliferation and apoptosis in several cell types [18,19] Recent data...

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Báo cáo sinh học: "Effect of ethanol on innate antiviral pathways and HCV replication in human liver cells" doc

Ngày tải lên : 19/06/2014, 08:20

... Materials and methods Cells and chemicals Human hepatoma Huh7 cells were grown in DMEM containing 10% FBS, 1× penicillin, streptomycin, fungizone, 10mM L-glutamine, and 1× non-essential amino acids ... signaling and increased HCV replication in the presence of IFN Inhibition of Jak-Stat signaling may involve ethanol perturbation of IFN-α induced changes in membrane fluidity, inhibition of IFN binding ... Ras-ERK, and phosphoinositol kinase (PI3K) [36-40] However, p38 kinase activity, which is important in IFN-α and IFN-γ induced transcription, is not involved in IFN induced Stat1 serine phosphorylation...

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báo cáo hóa học:"Effect of ethanol on innate antiviral pathways and HCV replication in human liver cells" potx

Ngày tải lên : 20/06/2014, 04:20

... Materials and methods Cells and chemicals Human hepatoma Huh7 cells were grown in DMEM containing 10% FBS, 1× penicillin, streptomycin, fungizone, 10mM L-glutamine, and 1× non-essential amino acids ... signaling and increased HCV replication in the presence of IFN Inhibition of Jak-Stat signaling may involve ethanol perturbation of IFN-α induced changes in membrane fluidity, inhibition of IFN binding ... Ras-ERK, and phosphoinositol kinase (PI3K) [36-40] However, p38 kinase activity, which is important in IFN-α and IFN-γ induced transcription, is not involved in IFN induced Stat1 serine phosphorylation...

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Báo cáo lâm nghiệp: "Regeneration and game damage in the Krušné hory Mts. assessed on the basis of National Forest Inventory of the Czech Republic" pps

Ngày tải lên : 07/08/2014, 03:22

... species in regeneration, shows damage of 32% of individuals in the investigated area (open space 34% of damaged individuals, below the stand 32% of damaged individuals) whilst average damage in the ... at summarizing the damage (browsing, peeling) and at expressing both types of damage together As average values from the preceding maps of browsing and peeling were used as a basis, damage is ... assessment of peeling and browsing intensity was carried out in two categories including individuals with dbh from cm to 11.9 cm and individuals with dbh from 12 cm to 20 cm Individuals with dbh...

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báo cáo khoa học: "Harmonic scalpel versus flexible CO2 laser for tongue resection: A histopathological analysis of thermal damage in human cadavers" potx

Ngày tải lên : 09/08/2014, 02:20

... cutting tool The ideal instrument would accomplish the necessary functions of cutting and coagulation while minimizing collateral tissue injury Decreasing overall tissue injury has obvious clinical ... first introduced into Otolaryngology by Jako and Strong in 1972 [7] At a wavelength of 10,600 nm, this laser is rapidly absorbed by the water in the tissues ensuring minimal thermal damage and ... and incisions were made in the tongue akin to a tongue tumor resection using the harmonic scalpel and flexible C02 laser fiber at recommended settings of 5W for the harmonic scalpel and settings...

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Báo cáo y học: "Intra-articular injection of recombinant TRAIL induces synovial apoptosis and reduces inflammation in a rabbit knee model of arthri" pdf

Ngày tải lên : 09/08/2014, 07:20

... to inducing apoptosis of the synovium, intra-articular injection of exogenous TRAIL protein reduced the leukocytic infiltrate into the inflamed joints, indicating that TRAIL may inhibit joint inflammation ... relatively inefficient in inducing synovial apoptosis, requiring a high multiplicity of infection to confer apoptosis in culture Thus it appears as if TRAIL, either administered as a recombinant protein ... transfer, is relatively ineffective in inducing apoptosis of both rabbit and human synovial fibroblasts in culture In contrast to the cell culture results using human and rabbit synovial fibroblasts,...

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Báo cáo khoa học: "Real time PCR analyses of expression of E-cadherin, alpha-, beta- and gamma-catenin in human breast cancer for predicting clinical outcome" pps

Ngày tải lên : 09/08/2014, 07:21

... following invasion and degradation of surrounding matrix, and migration into the vasculature and surrounding tissue, E-cadherin catenin complex is reintroduced and cells adhere to the vasculature and ... for positivity and negativity We determined the expression of E-cadherin, α-, β- and γcatenin in human breast carcinomas using real time quantitative polymerase chain reaction and investigated ... relationship of E-cadherin, α-catenin, β-catenin and γ-catenin in breast tumours to Nottingham Prognostic Index (NPI) (E-cadherin p = 0.094, αcatenin p = 0.144, β-catenin p = 0.378, γ-catenin p = 0.131)...

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Báo cáo khoa học: "Costunolide causes mitotic arrest and enhances radiosensitivity in human hepatocellular carcinoma cells" potx

Ngày tải lên : 09/08/2014, 09:20

... paraformaldehyde in PBS (pH 7.4) and mounted in Vectashield mounting medium with 1.5 Ag/mL 4V, 6-diamidino-2phenylindole (Vector Laboratories, Inc., Burlingame, CA) The cells were stained by method ... again with PBS and resuspended in PBS containing PI and RNase A The samples were subjected to a FACScaliber flow cytometer and data analysis was done using CellQuest Pro software (Becton Dickinson, ... separated apart and, instead, aggregated in the center of the nucleus in a round cell contour These findings indicated a mitotic arrest at the metaphase was induced by costunolide Signaling molecules...

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Báo cáo khoa học: " The radiosensitizer 2-benzoyl-3-phenyl-6,7-dichloroquinoxaline 1,4-dioxide induces DNA damage in EMT-6 mammary carcinoma cells" potx

Ngày tải lên : 09/08/2014, 10:20

... confluency DNA- binding proteins were cross-linked to DNA by adding 1% formaldehyde for 10 at 37°C Cells were washed twice with ice-cold PBS containing protease inhibitors (1 mM PMSF, μg/mL aprotinin and ... G2/M DNA- damage checkpoint [28] The G2/M arrest induced by DCQ and the S-phase accumulation induced by IR appear together in combination treatments Despite our intriguing findings that the combination ... was cultured in growth media containing RPMI 1640 with L-glutamine and 25 mm HEPES, supplemented with 10% FBS and 1% penicillin-streptomycin (50 μg/mL), and incubated in a humidified incubator (95%...

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Báo cáo y học: "Association of elevated transcript levels of interferon-inducible chemokines with disease activity and organ damage in systemic lupus erythematosus patients" docx

Ngày tải lên : 09/08/2014, 13:22

... chemokine ligand; CXCL, C-X-C chemokine ligand; IFIG, IFN-inducible gene; IFIT, interferon-induced protein with tetratricopeptide repeats; IL, interleukin; IP-10, interferon-inducible protein 10; ... chemokine scores in SLE patients with organ damage Given that chemokines are involved in tissue damage and inflammation, we next explored whether chemokine scores are related to organ damage in ... Mampaso F, Schlondorff D: Chemokine expression precedes inflammatory cell infiltration and chemokine receptor and cytokine expression during the initiation of murine lupus nephritis J Am Soc Nephrol...

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Báo cáo y học: "T-614, a novel immunomodulator, attenuates joint inflammation and articular damage in collagen-induced arthritis" docx

Ngày tải lên : 09/08/2014, 13:22

... remained almost intact The findings support the view that T614 can protect the joints from damage in an inflammatory environment, in concert with MTX Proinflammatory cytokines TNF-α, IL-1β, and ... disease was indicated by increased edema and erythema of one or both ankle joints, followed by involvement of the metatarsal and interphalangeal joints Fully developed arthritis, including red and swollen ... sharply in the PBMCs from highdose T-614 and combination treated rats In particular, T-614 inhibited the elevated IL-17 expression in inguinal lymph node cells in a dose-dependent manner IFN-γ and...

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Báo cáo y học: "erum levels of soluble receptor for advanced glycation end products and of S100 proteins are associated with inflammatory, autoantibody, and classical risk markers of joint and vascular damage in rheumatoid arthritis" doc

Ngày tải lên : 09/08/2014, 13:22

... Fagerhol MK, Landewe R, Heijde D van der, Uhlig T, Mowinckel P, Kvien TK: Calprotectin (a major leucocyte protein) is strongly and independently correlated with joint inflammation and damage in rheumatoid ... relationship to joint and vascular damage and risk factors in RA patients [19] We report associations of each protein with both joint and vascular disease and their risk factors Materials and methods ... erosions and joint space narrowing using the modified Sharp score [47] Genotyping High resolution human leukocyte antigen (HLA)-DRB1 genotyping was carried out on buffy coat DNA using PCR and sequence-specific...

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Báo cáo y học: " Basal core promoters control the equilibrium between negative cofactor 2 and preinitiation complexes in human cells" pdf

Ngày tải lên : 09/08/2014, 20:21

... formation and TFIIB binding in vivo The high prevalence for the occurrence of motif combinations in TFIIB-dominated genes in illustrated in Figure 5e In line with the known synergy between INR and ... (black line) and replicate (grey line) relative to aligned TSSs at 10-bp resolution Binding of TBP and the initiating form of RNAPII (phosphorylated at serine in its carboxy-terminal domain) were ... one initial cycle consisting of minutes at 55° (during which polymerase was added), minutes at 72°C and minutes at 94°C, followed by 22 cycles of 0.5 minutes at 94°C, 0.5 minutes at 60°C and minutes...

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Báo cáo y học: "Active DNA demethylation in human postmitotic cells correlates with activating histone modifications, but not transcription levels" ppt

Ngày tải lên : 09/08/2014, 20:22

... pyruvate (Invitrogen), MEM vitamins (Invitrogen), 50 U/ml penicillin/streptomycin (Invitrogen), and 50 nM 2-mercaptoethanol (Invitrogen) The human cervical carcinoma cell line HeLa was maintained in ... model in which the TATA box binding protein-associated factor TAF12 recruits Gadd45a (growth arrest and DNA- damage induced-a) and the nucleotide excision repair machinery to promoters, resulting in ... Recombinant human interleukin-3 and granulocytemacrophage colony-stimulating factor show common biological effects and binding characteristics on human monocytes Blood 1989, 74:2349-2359 25 Finnin M,...

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Báo cáo y học: "LMP-420, a small-molecule inhibitor of TNF-alpha, reduces replication of HIV-1 and Mycobacterium tuberculosis in human cell" pps

Ngày tải lên : 10/08/2014, 05:20

... cDNA prepared and RT-PCR performed for 30 cycles using primers for TNF-α and β-actin obtained from R & D Systems Thus, several studies suggest that minimizing TNF levels in both HIV and M Tb-infected ... TNF inhibited p24 release induced by mycobacteria In a recent review Collins et al [4] postulate that higher viral loads, increased HIV diversity, and changes in cytokine/chemokine levels in HIV-infected ... goes untreated in HIV+ patients, most will die within one year M Tb is the most common opportunistic infection occurring in HIV-infected individuals in resource poor countries and it accelerates...

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Báo cáo y học: "JNK suppression is essential for 17b-Estradiol inhibits prostaglandin E2-Induced uPA and MMP9 expressions and cell migration in human LoVo colon cancer cells" ppsx

Ngày tải lên : 10/08/2014, 10:20

... significantly inhibits PGE-induced activation of JNK1/2 within 30 min, and suppressed PGE2-induced expression of uPA and MMP-9 within 24 h in human LoVo colon cancer cells (Figure 4B) 17b-Estradiol Inhibits ... of proteins by immunoblotting assay Phosphorylation of JNK1/2 was significantly induced within 15 in response to PGE2 stimulation, and was maintained up for 24 h (Figure 3B) The findings suggested ... functionally related zinc-containing enzymes that include interstitial collagenases, gelatinases, stromelysin, matrilysin, metalloelastase, and membrane-type MMPs [7,8] Upregulation of MMP-2 and MMP-9 has...

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