and off target pharmacology or selectivity of protein kinase inhibitors
Tài liệu Comparison of lung cancer cell lines representing four histopathological subtypes with gene expression profiling using quantitative real-time PCR pptx
Ngày tải lên :
15/02/2014, 04:20
... with the expression of RAB25 and AMY2A, and positively correlated with TGM2, MALL, and IGSF3 Gene networks and gene pathways To further understand the biological networks of the 19 genes, we ... about the reliability of microarray technology for analyzing differential expression, because of the lack of reproducibility across laboratories and platforms despite the use of highly similar protocols ... TGM2, and positively correlated with IGSF3, STMN1, FOXG1, and RAB25 The loading number of component (PC2) was negatively correlated with the expression of STMN1, AMY2A, and ISL1, and positively correlated...
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Tài liệu Báo cáo khoa học: A differential scanning calorimetry study of tetracycline repressor pptx
Ngày tải lên :
20/02/2014, 02:21
... concentration of 0.6 mgặmL)1 and scan rate (v) of 0.12.0 Kặmin)1 Measurements of liganded protein were performed in buffer B, at a protein concentration of 0.4 mgặmL)1 and at vefold molar excess of Tc ... Tmax, of the complex of TetR with Tc is 70.4 C, measured at a protein concentration of 0.4 mgặmL)1 and scan rate of Kặmin)1 (Figs and 2) Therefore, under these experimental conditions, binding of ... fraction of the protein in the temperature function, fn and fu are fractions of native protein for pre-transition and post-transition curves, respectively, obtained by extrapolation to K, and mn and...
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Applications of Calorimetry in a Wide Context - Differential Scanning Calorimetry, Isothermal Titration Calorimetry and Microcalorimetry docx
Ngày tải lên :
06/03/2014, 22:20
... slopes of 0.65 and –0.20 for short and extended milling times, respectively, in the case of Fe; and slopes of –0.85 and –0.03, respectively, for short and extended milling times in the case of Fe50Co50 ... editor of the book for my experience and publications in the field of applications of calorimetry and biocalorimetry in the analysis of small and large drug molecules I welcomed the invitation and ... Determination of Folding Reversibility of Lysozyme Crystals Using Microcalorimetry 185 Amal A Elkordy, Robert T Forbes and Brian W Barry Chapter Calorimetric Study of Inulin as Cryo- and Lyoprotector of...
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Báo cáo khoa học: Purification and structural study of the b form of human cAMP-dependent protein kinase inhibitor pdf
Ngày tải lên :
07/03/2014, 15:20
... localization and hormonal regulation of the PKI and PKI isoforms of the inhibitor protein of the cAMP-dependent protein kinase J Biol Chem 272, 20011–20020 Byler, D.M & Susi, H (1986) Examination of the ... Evidence for two additional isoforms of the endogenous protein kinase inhibitor of cAMP-dependent protein kinase in mouse J Biol Chem 268, 10927–10931 Olsen, S.R & Uhler, M.D (1991) Inhibition of protein ... isoform of the protein kinase inhibitor of cAMPdependent protein kinase J Biol Chem 272, 18169–18178 Gamm, D.M & Uhler, M.D (1995) Isoform-specific differences in the potencies of murine protein- kinase...
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Báo cáo Y học: Differential scanning calorimetric study of myosin subfragment 1 with tryptic cleavage at the N-terminal region of the heavy chain pdf
Ngày tải lên :
17/03/2014, 10:20
... is of crucial importance for the structural integrity of the myosin head An important role of the N-terminal region of the myosin head in the communication between the motor domain and regulatory ... effects of the formation of the ternary complexes of t-S1 and Nt-S1 with ADP-Vi and ADP-BeFx, in comparison with the proteins containing ADP alone (control curves shown by dashed lines) For both proteins, ... was used for calculation of DHcal for all proteins studied Protein experiments performed under the same conditions, at the same protein concentration (1 mgÆmL)1), and even in the same rotor Both...
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selfridge, arnold, warnick. teaching electromagnetic field theory using differential forms
Ngày tải lên :
24/04/2014, 16:51
... 3-forms are both one-dimensional, and the spaces of 1-forms and 2-forms are both three-dimensional The star operator ? is a set of isomorphisms between these pairs of vector spaces For 1-forms and ... examples of forms of various degrees The coe cients of the forms can be functions of position, time, and other variables TABLE I Differential forms of each degree Degree 0-form 1-form 2-form 3-form ... 2], and Burke 3] These works note some of the advantages of the use of di erential forms in EM theory Misner et al and Burke treat the graphical representation of forms and operations on forms,...
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Differential scanning calorimetrycalorimetry
Ngày tải lên :
24/05/2014, 20:41
... data and Applications – Real events – Artefacts Recent advances EYP 2006 Calorimetry Calorimetry – The study of heat transfer during physical and chemical processes Calorimeter – A device for measuring ... not necessarily show interaction at room temp EYP 2006 Polymorph Screening and Indentification Form II Form Form II Form II Variable Form III Hydrate Dihydrate Acetic acid solvate 1.0 0.5 0.0 Heat ... polymorph – melting observed Lot B: seeds of high melting polymorph induce solid-state transition below the melting temperature of the low melting polymorph EYP 2006 Outline Brief history of thermal...
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báo cáo hóa học:" Preclinical evaluation of dasatinib, a potent Src kinase inhibitor, in melanoma cell lines" pptx
Ngày tải lên :
18/06/2014, 15:20
... c-KIT, PDGFR and ephrin-A receptor kinases It is the most potent Src kinase inhibitor currently in clinical development with an IC50 of 0.5 nM for Src kinase (IC50 of < 30 nM for the other targets) ... sensitivity of melanoma cell lines to dasatinib may be due to targeting Src kinase or EphA receptors, which are not targeted by imatinib Differences in the level or phosphorylation of Src kinase not ... Effect of dasatinib on Src kinase, EphA2 and FAK Src, EphA2, FAK and phosphorylated Src, EphA2 and FAK were detected in all cell lines tested, although the levels of phosphorylated Src kinase...
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Báo cáo hóa học: " Temperature-Dependent Site Control of InAs/GaAs (001) Quantum Dots Using a Scanning Tunneling " pptx
Ngày tải lên :
21/06/2014, 08:20
... of a dot grown in a hole structure under In and As4 irradiations at 300°C and its line profiles a Shows the hole structure, b and c were images after supplying additional 0.02 ML and 0.04 ML of ... any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited References Fig Fabrication process of a nano-dot growth under As4 irradiation ... from left to right and moved from bottom to top of the image After a thermal drift reducing, using the STM tip, we applied voltage ‘from a reverse bias to a forward bias’ The time of applying voltage...
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Báo cáo vật lý: "EXOTHERMIC TRANSITIONS ON COOLING OF GELATINIZED NATIVE RICE STARCH STUDIED BY DIFFERENTIAL SCANNING CALORIMETRY" doc
Ngày tải lên :
07/08/2014, 14:20
... transitions were broad and flatten with Tcxo of ~119oC for 1:1, sharpen prominently and bigger for 1:2 but became slightly smaller and broader for 1:3, with Tcxo of ~89oC and 85oC, respectively ... transition of amylose-lipid complex is heatreversible.9,10 Therefore, our observations on Tcxo ranged from 83oC to 94oC for 1:3 and 1:2 systems, and 109oC–120oC for 1:1 system conformed with ... (type II) are readily formed during cooling of 1:1 starch-water system in which annealing and recrystallization are likely to occur For 1:2 and 1:3 systems, a decrease of Tcxo and Tcx with decrease...
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Báo cáo y học: " Local treatment with the selective IκB kinase β inhibitor NEMO-binding domain peptide ameliorates synovial inflammation" pot
Ngày tải lên :
09/08/2014, 08:22
... non-fat dry milk (Bio-Rad Laboratories, Inc.), Na3VO4 (2 mM), and 0.05% Tween 20 for hour Detection of phosphorylated (ph) and unphosphorylated proteins was performed by incubating the membranes ... glucocorticoid receptor and nuclear factor-kappaB or activator protein- 1: molecular mechanisms for gene repression Endocr Rev 2003, 24:488-522 Weitoft T, Larsson A, Saxne T, Ronnblom L: Changes of ... dose of the NBD peptide for amelioration of arthritis, animals (n = 6/group) were treated intra-articularly at the onset of arthritis (day 10) and days later (day 12) with either 50 or 150 µg of...
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Báo cáo y học: "Metalloproteinase and inhibitor expression profiling of resorbing cartilage reveals pro-collagenase activation as a critical step for collagenolysis" pot
Ngày tải lên :
09/08/2014, 08:22
... synthesis and activation of pro-collagenases, causing almost complete resorption of human and bovine nasal cartilage in a short assay period [19,20] Natural and synthetic metalloproteinase inhibitors ... compare the temporal expression of metalloproteinases and their inhibitors and correlate this with pro-collagenase activation and aggrecan and collagen release Materials and methods Cartilage degradation ... we have profiled for the first time the gene expression of multiple metalloproteinases and their inhibitors in actively resorbing cartilage by real-time Page of 12 (page number not for citation...
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Báo cáo khoa học: " The c-Met receptor tyrosine kinase inhibitor MP470 radiosensitizes glioblastoma cells" ppt
Ngày tải lên :
09/08/2014, 10:20
... design and provided resource to perform this work RE performed some of the experiments LC provided technical laboratory support DB provided resource and technical advice BS provided resources and ... design and coordination All authors read and approved the final manuscript 10 11 Acknowledgements This work was supported by a grant from SuperGen and we would like to thank their support of our work ... HGF or c-Met expression using ribozyme radiosensitizers in GBM cells in vitro and xenograft tumor in vivo [10], demonstration of clinically useful inhibitors of the tyrosine kinase activity of...
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Báo cáo y học: "Reliability of capturing foot parameters using digital scanning and the neutral suspension casting technique" ppt
Ngày tải lên :
10/08/2014, 21:24
... exception of forefoot to rearfoot alignment The SRD results are reported in Table and The SRD value for forefoot to rearfoot alignment was 1.24° for rater and 1.50° for rater with digital scanning and ... 0.81-0.99 for all foot parameters SEM values ranged from 0.30-1.13 mm for rater and 0.401.13 mm for rater SEM values for rearfoot to forefoot alignment were 0.45° for rater and 0.54° for rater ... Evaluation and management of foot and ankle disorders: present problems and future directions J Orthop & Sports Phys Ther 1995, 29:381 16 Payne C: Cost benefit comparison of plaster casts and optical...
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báo cáo khoa học: "ABT-869, a promising multi-targeted tyrosine kinase inhibitor: from bench to bedside" doc
Ngày tải lên :
10/08/2014, 22:20
... number of kinases relevant to the VEGF signaling pathway AG013736, CHIR258, and SU11248 are also active against most of the targeted kinases but these inhibitors demonstrate more off- target activity ... expression of VEGF and its receptors are detected in most of solid tumors and hematological malignancies [13] Overexpression of VEGF and/ or it's receptor VEGFR2 contributes to invasiveness and metastasis ... http://www.jhoonline.org/content/2/1/33 Figure Kinase inhibition profile of ABT-869 against a broader range of kinases Kinase inhibition profile of ABT-869 against a broader range of kinases Nonclinical...
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Báo cáo y học: "Inhibitory effects of ZSTK474, a novel phosphoinositide 3-kinase inhibitor, on osteoclasts and collagen-induced arthritis in mice" pot
Ngày tải lên :
12/08/2014, 12:20
... signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase (MAPK) [10-14] In OCs, PI3-K is a major downstream effecter of the M-CSF receptor, RANK, and αβν3 integrin The importance of PI3-K for ... and transferred onto dentin slices and incubated for 72 hours in the presence or absence of ZSTK474 and other PI3-K inhibitors The dentin slices were stained with hematoxylin, and the pits formed ... survival and motility of OCs has been demonstrated by using the PI3K inhibitors wortmannin and LY294002 [15-22], and also by studying mice deficient in the expression of the p85α subunit of class...
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Báo cáo y học: " Inhalation of the Rho-kinase inhibitor Y-27632 reverses allergen-induced airway hyperresponsiveness after the early and late asthmatic reaction" pps
Ngày tải lên :
12/08/2014, 16:20
... protein levels of this upstream activator of Rho -kinase have been reported ele- Page of (page number not for citation purposes) Respiratory Research 2006, 7:121 http://respiratory-research.com/content/7/1/121 ... MLCK and MLCP activities by phosphorylating and thereby inactivating the myosin binding subunit of MLCP This leads to an augmentation of MLC20 phosphorylation and hence an elevated level of contraction ... histamine and PGF2α before and after the allergen-induced EAR and LAR We demonstrate that there is a differential role of Rho -kinase in the agonistinduced airway obstructions and that inhalation of...
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Báo cáo sinh học: "Characteristics of predictor sets found using differential prioritization" docx
Ngày tải lên :
12/08/2014, 17:20
... The aim of toy datasets is to provide simple but clear and demonstrative examples on the importance of the correct choice of the value of the DDP in forming the best predictor set Furthermore, another ... section Standard deviation among samples of the same class, or class standard deviation, is set to for all instances, σi,k = for all k and i For all k, a total of m samples are generated for class ... techniques on the concept of relevance and redundancy having equal importance in the formation of a good predictor set We call the predictor set scoring methods used in such correlation-based feature...
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