and 10 amino acid peptides spanning ie 11 15 and ie 15 19

Tài liệu Báo cáo khoa học: Looking for the ancestry of the heavy-chain subunits of heteromeric amino acid transporters rBAT and 4F2hc within the GH13 a-amylase family ppt

Ngày tải lên : 18/02/2014, 13:20

... 8, 3809–3 819 Lee WS, Wells RG, Sabbag RV, Mohandas TK & Hediger MA (199 3) Cloning and chromosomal localization of a human kidney cDNA involved in cystine, dibasic, and neutral amino acid transport ... of enzymes Biochim Biophys Acta 154 6, 1–20 Wells RG & Hediger MA (199 2) Cloning of a rat kidney cDNA that stimulates dibasic and neutral amino acid transport and has sequence similarity to glucosidases ... Heteromeric amino acid transporters: biochemistry, genetics, and physiology Am J Physiol Renal Physiol 281, 995 101 8 Broer S & Wagner CA (2002) Structure–function relationships of heterodimeric amino acid...

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Tài liệu Báo cáo khoa học: Puriﬁcation and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx

Ngày tải lên : 18/02/2014, 17:20

... an elution gradient of 0 10 100% Buffer A, 10 20 0–27% Buffer B, 20–25 27% Buffer B, 25–58 27–42.5% Buffer B, and 58–63 42.5 100 % Buffer B Buffer A is 0.1% triﬂuoroacetic acid and Buffer B is ... Trp14 and Thr15 The observation of two small 3JHN-Ha coupling constants for residues Asn11 and Ser12 and the dNN(i,i + 2), daN(i,i + 2) [Ser12(CaH)–Trp14(NH) and Asn11(CaH)–Phe13(NH)] and daN(i,i ... pointing out that the l -amino acid to d -amino acid epimerization seems to be complete for conopeptides, whereas both isoforms coexist in defensin-like peptides and natriuretic peptides from the Australian...

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Tài liệu Báo cáo khoa học: Implication of the glutamine synthetase ⁄glutamate synthase pathway in conditioning the amino acid metabolism in bundle sheath and mesophyll cells of maize leaves doc

Ngày tải lên : 18/02/2014, 18:20

... 15 18 21 24 100 L-FNR Fd VI 12 15 18 21 24 100 100 50 50 12 15 18 21 24 100 100 100 12 15 18 21 24 50 12 15 18 21 24 100 50 100 GLU Gln2 50 Gln1-3 100 50 0 gdh1 12 15 18 21 24 Fd III 50 L-FNR 3 ... 12 15 18 21 24 100 Fd II 50 12 15 18 21 24 6 12 15 18 21 24 Time of day (h) 50 6 12 15 18 21 24 Time of day (h) 12 15 18 21 24 12 15 18 21 24 50 12 15 18 21 24 100 50 50 0 3 50 12 15 18 21 24 100 ... 0.0 12 15 18 21 24 12 15 18 21 24 Asp F 20 0.6 15 15 0.4 10 10 0.2 5 0 0.0 12 15 18 21 24 Ala G H 12 15 18 21 24 Gly 50 Relative amount % C Relative amount % 25 15 Gln Ammonium A 20 10 12 15 18...

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Tài liệu Báo cáo khóa học: Active-site residues and amino acid speciﬁcity of the bacterial 4¢-phosphopantothenoylcysteine synthetase CoaB pptx

Ngày tải lên : 19/02/2014, 12:20

... N 210 is an active-site residue The mutant His-CoaB proteins N210A, N210E, N210H, N210K and N210Q formed dimers as had also been observed for the wt enzyme ([5]; Fig 4) Residues R206, N 210 and ... American Elsevier, New York Lynen, F (196 3–70) The Pathway from ‘Activated Acetic Acid to the Terpenes and Fatty Acids in Nobel Lectures: Physiology or Medicine, pp 103 –138, American Elsevier, New ... His-CoaB N210K and copuriﬁcation of the 4¢-phosphopantothenoylCMP intermediate His-CoaB proteins (His-CoaB and His-CoaB N210K) were puriﬁed from E coli cell extracts by Ni-nitrilotriacetic acid and...

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Tài liệu Báo cáo khoa học: Local stability identiﬁcation and the role of a key aromatic amino acid residue in staphylococcal nuclease refolding pdf

Ngày tải lên : 20/02/2014, 01:20

... Structure, Function and Bioinformatics 56, 271–277 Gill SC & von Hippel H (198 9) Calculation of protein extinction coefﬁcients from amino acid sequence data Anal Biochem 181, 319 326 FEBS Journal ... W140O and K133A] The curves of W140A and W140O are nearly linear in terms of intensity All protein concentrations were mgÆmL)1 of the wild-type protein and K133A and E142O were 88.45, 84.70, and ... stability identiﬁcation and the role of key acidic amino acid residues in staphylococcal nuclease unfolding FEBS Journal 272, 3967–3974 Chen HM, Markin VS & Tsong TY (199 2) pH induced folding...

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Tài liệu Báo cáo Y học: Importance of the amino-acid composition of the shutter region of plasminogen activator inhibitor-1 for its transitions to latent and substrate forms pdf

Ngày tải lên : 22/02/2014, 07:20

... stressed and the relaxed conformations [15] The network involves the side chains of the amino acids in positions 53 and 56 in a helix B, 186 in b strand 3A, and position 334 in b strand 5A (Fig ... b strand 6A and the backbone of Asn171 in the a helix F/b strand 3A loop Note: SWISSPDB VIEWER uses the same signature for a helices and the short 310- helix found in the a helix F/b strand 3A ... number of substitutions in the shutter region and b strand 5A of PAI-1 and studied their effect on the transition to latent and substrate forms and on the stabilizing effect of vitronectin, a...

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Tài liệu Báo cáo Y học: Bivalent cations and amino-acid composition contribute to the thermostability of Bacillus licheniformis xylose isomerase doc

Ngày tải lên : 22/02/2014, 07:20

... 3.52 9.40 Lys 0.61 0.70 0.27 0.52 10. 93 10. 43 9.76 10. 10 11. 18 10. 56 10. 50 9.64 10. 77 0.4 0.63 10. 02 10. 21 10. 36 9.38 10. 25 9.49 11. 38 10. 57 Leu 0.27 0.92 0.07 0.26 2.12 2.42 2.46 2.17 2.28 2.86 ... recent studies [9,47–49] All these studies reported a decrease in the content of uncharged polar amino acids (i.e Q, N, S, and T) and an increase in charged amino- acid residues (i.e K, E, and R) ... 10. 45 8.50 7.25 5 .19 7.32 Ile 2.08 0.75 3.49 1.87 4.26 6.81 5.57 8.38 8.94 4.48 6.32 7.05 9.48 4.02 2.00 7.61 7.61 7.74 10. 36 7.80 6.86 3.52 9.40 Lys 0.61 0.70 0.27 0.52 10. 93 10. 43 9.76 10. 10...

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Báo cáo khoa học: Amino acid limitation regulates the expression of genes involved in several speciﬁc biological processes through GCN2-dependent and GCN2-independent pathways ppt

Ngày tải lên : 07/03/2014, 03:20

... (197 4) Serum proteins and plasma free amino acids in severe malnutrition Am J Clin Nutr 27, 733–742 Gietzen DW (199 3) Neural mechanisms in the responses to amino acid deﬁciency J Nutr 123, 610 625 ... 40, 91 109 10 Iraqui I, Vissers S, Andre B & Urrestarazu A (199 9) Transcriptional induction by aromatic amino acids in Saccharomyces cerevisiae Mol Cell Biol 19, 3360–3371 11 Hinnebusch AG (198 8) ... to amino acid availability has been studied extensively in yeast [8] The GCN2 and TOR kinases sense the intracellular concentration of amino acids In addition, yeast cells possess an amino acid...

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Báo cáo khoa học: Phytoene synthase genes in tomato (Solanum lycopersicum L.) – new data on the structures, the deduced amino acid sequences and the expression patterns doc

Ngày tải lên : 07/03/2014, 05:20

... Integral Amino Length 5¢-UTR CDS 3¢-UTR Length acids Psy1 32 656 51 173 236 193 181 942 120 423 313 518 689 4527 Psy2 112 710 51 173 236 193 199 153 107 710 273 227 398 3542 a Protein Exons and were ... whiteﬂower locus Plant Cell 18, 194 7 196 0 Ray J, Bird C, Maunders M, Grierson D & Schuch W (198 7) Sequence of pTOM5, a ripening related cDNA from tomato Nucleic Acids Res 15, 105 87 Bartley GE, Viitanen ... yellow and the fruit is red? G Giorio et al Fig Sequence alignment of PSY1 and PSY2 protein derived from analysis with The predicted protein sequences were 412 and 438 amino acids for PSY1 and PSY2,...

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Báo cáo khoa học: Substrate speciﬁcity and transport mode of the proton-dependent amino acid transporter mPAT2 potx

Ngày tải lên : 07/03/2014, 16:20

... as < 5% Uptake of L-[3H]proline (100 lM) was measured at pH 6.5 for 10 in the presence of unlabeled amino acids and derivatives at a ﬁxed concentration of 10 mM and are given as percentage inhibition ... N (199 2) Cloning and expression of a glycine transporter from mouse brain FEBS Lett 305, 110 114 16 Mackenzie, B., Loo, D.D., Fei, Y., Liu, W.J., Ganapathy, V., Leibach, F.H & Wright, E.M (199 6) ... set of amino acids and derivatives divided into different categories with differences in (a) side chain size, (b) backbone length, (c) O- and N-methyl substitutions, (d) stereochemistry and (e)...

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Báo cáo khoa học: Local stability identiﬁcation and the role of key acidic amino acid residues in staphylococcal nuclease unfolding ppt

Ngày tải lên : 07/03/2014, 21:20

... with H121 and K9, and of G129 with K 110 and K133 Other charged amino acids such as D73, D83 and E101 reinforce the interactions of E75 and E129 ˚ (OE1 ⁄ OE2)– are K 110 (Nz)+ (¼ 6.29 A) and K133 ... namely helix (V99 to Q106) and helix (E122 to K134) This ﬁnding implies that E129 and E101 form another local stable area through interactions with K 110 and K133 (Fig 4) and is in agreement with ... Local stability and key acidic amino acids in staphylococcal nuclease by the interactions of E75 with H121 and K97 It is believed that a small number of key amino acid segments play major...

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Báo cáo khoa học: Structural and biological effects of a b2- or b3-amino acid insertion in a peptide Application to molecular recognition of substance P by the neurokinin-1 receptor ppt

Ngày tải lên : 08/03/2014, 08:20

... three-dimensional structures of a -amino acids and those of b -amino acids and posed the following questions Which structures of b -amino acids overlap the canonical structures of a -amino acids? Subsequently, ... b -amino acid [19] , whereas the introduction of two adjacent b -amino acids induced a 10- membered hydrogen-bonded turn (C10) analogous to the b-turn made of a -amino acids [20] This propensity of b -amino ... representation of a-, b2- and b3 -amino acids with the torsion angles /, h and w For the b2 and b3 -amino acid substituted-SP analogues, the methyl side chain of b-HAla exhibits the same orientation as the...

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Báo cáo Y học: Identiﬁcation and characterization of a new gene from Variovorax paradoxus Iso1 encoding N -acyl-D-amino acid amidohydrolase responsible for D-amino acid production pdf

Ngày tải lên : 08/03/2014, 16:20

... Purification and properties of D-aminoacylase of Streptomyces olivaceus Agric Bio Chem 42, 107 113 Ó FEBS 2002 19 Tsai, Y.C., Tseng, C.P., Hsiao, K.M & Chen, L.Y (198 8) Production and purification of D-aminoacylase ... T & Fukagawa, Y (198 0) Deacetylation of PS-5, a new beta-lactam compound II Separation and purification of L -amino acid acylase and D -amino acid acylase from Pseudomonas sp 115 8 J Antibiotic 33, ... of free amino acids and amino acid residues in proteins by radiolysis and by metal-catalyzed reactions Ann Rev Biochem 62, 797–821 53 Stadtman, E.R (199 2) Protein oxidation and aging Science 257,...

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Báo cáo khoa học: Mutational analyses of human eIF5A-1 – identiﬁcation of amino acid residues critical for eIF5A activity and hypusine modiﬁcation doc

Ngày tải lên : 16/03/2014, 06:20

... L91A, L101A, P115A, E116A, L119A, E144A, K150A, M43A ⁄ M79A, and C73A ⁄ M79A, were generated using the QuickChange Sitedirected Mutagenesis Kit and employing p414GAL1 ⁄ heIF5A-1 and pET11a ⁄ heIF5A-1 ... K50R, H51A, G52A, K55A, F64A, P74A, H77A, M79A, P82A, I84A, R86A, L91A, L101A, P115A, E116A, L119A, E144A, K150A, M43A ⁄ M79A, and C73A ⁄ M79A The ability of each mutant protein to complement growth ... 5.2 (K47A), 113 .8 ± 10. 8 (K47R), 238.7 ± 11. 3 (H51A), 144.5 ± 14.2 (P74A), 150 .1 ± 12.5 (L91A) and 444.0 ± 32.6 (L101A), whereas that for the strain expressing wild-type heIF5A was 112 .3 ± 6.8...

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Báo cáo khoa học: Structural and functional consequences of single amino acid substitutions in the pyrimidine base binding pocket of Escherichia coli CMP kinase pdf

Ngày tải lên : 16/03/2014, 10:20

... or dCMP showed that discrimination between CMP and UMP is achieved by Ser36, Arg 110 and Asp132, which form hydrogen bonds with the amino group and the N3 atom of the cytosine (Fig 1) This study ... Results Overproduction and molecular characterization of the modied variants of E coli CMP kinase The wild-type and various modied forms (S36A, R110M, D132A, D132H, D132N, D132S and R188M) of E coli ... M-D (199 0a) Mechanism of adenylate kinase Critical evaluation of the X-ray model and assignment of the AMP site Biochemistry 29, 109 5 6109 64 19 Tickle IJ, Laskowski RA & Moss DS (199 8) Rfree and...

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Báo cáo khoa học: Characterization of D-amino-acid-containing excitatory conotoxins and redeﬁnition of the I-conotoxin superfamily pot

Ngày tải lên : 16/03/2014, 22:20

... r11c (r11c N); middle panel, a mixture of two synthetic variants, r11c (r11c S) and [L-Leu42]r11c; bottom panel, a mixture of natural and synthetic r11c (r11c N and r11c S) Elutions were carried ... to the d version of the peptides simply as r11b and r11c, and the all-l versions as [l-Phe44]r11b and [l-Leu42]r11c Increased biological potency of D -amino- acidcontaining peptides A whole animal ... (presence of D -amino acid unlikely) C radiatus R11.3 C betulinus Bt11.1 C episcopatus Ep11.1 Group II C C betulinus BtX ⁄ BtX C virgo ViTx ⁄ ViTx C C C C Em11 .10 Fi11 .11 Ep11.12 Sx11.2 4184 emaciatus...

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Báo cáo khoa học: Substrate speciﬁcity of the human UDP-glucuronosyltransferase UGT2B4 and UGT2B7 Identiﬁcation of a critical aromatic amino acid residue at position 33 doc

Ngày tải lên : 23/03/2014, 09:21

... loading onto the gel 2, 10 and 100 lg of membrane proteins for UGT2B4, UGT2B4F33L and UGT2B4F33Y, respectively, and 12, 15 and 15 lg of membrane proteins for UGT2B7, UGT2B7Y33L and UGT2B7Y33F, respectively ... Mackenzie PI & Miners JO (199 7) The regioand stereo-selectivity of C19 and C21 hydroxysteroid glucuronidation by UGT2B7 and UGT2B11 Arch Biochem Biophys 341, 207– 211 13 Villeneuve L, Girard H, Fortier ... UGT2B7Y33F and UGT2B4 and 200 lg for UGT2B4F33Y and UGT2B4F33L in 100 mm Tris ⁄ HCl buffer (pH 7.4), 10 mm MgCl2 containing 0.02 mm UDP-glucuronic acid and 0.1 lCi UDP-[U-14C]glucuronic acid The...

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Báo cáo khoa học: Amino acid biosynthesis and metabolic ﬂux proﬁling of Pichia pastoris ppt

Ngày tải lên : 23/03/2014, 12:20

... Ministry of Science and Technology (CICYT project PPQ2001 -190 8), and the Academy of Finland (projects 52 311 and 202409) The authors thank J M Cregg and C Gancedo for useful comments, and O Cos for ... C (2000) Carbohydrate and energy-yielding metabolism in non-conventional yeasts FEMS Microbiol Rev 24, 507–529 12 Jones, E.W & Fink, G.R (198 2) Regulation of amino acid and nucleotide biosynthesis ... (199 8) 13C-NMR, MS and metabolic ﬂux balancing in biotechnology research Q Rev Biophys 31, 41 106 Szyperski, T (199 5) Biosynthetically directed fractional 13C-labeling of proteinogenic amino acids...

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Báo cáo khoa học: Surface exposed amino acid differences between mesophilic and thermophilic phosphoribosyl diphosphate synthase ppt

Ngày tải lên : 23/03/2014, 13:20

... discrepancies Lys289 and Arg294 were found to be glutamic acid and alanine, respectively The codon for Val292 was found to be GUG and not GUC as published originally [19] Temperature and pH dependency ... shading, a second dimer with green and purple shading and a third dimer with blue and yellow shading Red atoms indicate amino acids that dier among B caldolyticus and B subtilis PRibPP synthases ... tted to Eqn and the following values were obtained: KATP 310 110 lM, KRib5P 530 140 lM and Vmax 440 69 lmolặmin)1ặmg protein)1 Assay of enzyme activity in the presence of a variety of nucleotides...

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Báo cáo Y học: The effect of amino-acid substitutions I112P, D147E and K152N in CYP11B2 on the catalytic activities of the enzyme pdf

Ngày tải lên : 24/03/2014, 03:21

... Corresponding amino acids of human CYP11B2 and CYP11B1 as well as mouse and rat CYP11B1 at the positions selected for mutagenesis Position Human CYP11B2 Human CYP11B1 Mouse and rat CYP11B1 112 147 152 ... contribute to efﬁcient CYP11B1 and CYP11B2 wild-type activity In addition, it is known from the crystal structures of CYP101, CYP108 and CYP102 that the N-terminal region encodes an amino- acid sequence ... substitutions at positions 147 and 152 (double mutant D147E/K152N), and 112 , 147 and 152 (triple mutant I112P/D147E/K152N) gave rise to cortisolproducing activity increases of 1.6-fold and 2.5-fold, respectively,...

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