... periodically expressed in the human cell cycle and their expression in tumors Mol Biol Cell 2002, 13:1977-2000 Cooper S: G1 and S phase geneexpression cannot be analyzed in mammalian cells synchronized ... means growing cells in one case at 25°C and in another case at 37°C and seeing what happens to them or what is different in these different conditions Or one may have Medium A and Medium B and ... experiment Summary The work of Liu, Gaido and Wolfinger [1] is a statistical analysisof a well-regarded paper [2] on the pattern ofgeneexpression during the cell cycle of eukaryotic cells There...
... remaining genes are found in the categories 'organelle organization and biogenesis' and 'intra- cellular protein transport' (Table 2) The expressionof genes involved in the ubiquitin cycle and protein ... autopsy and resection samples Geneexpression profiles were determined in six resection samples from hippocampus and frontal cortex, and in four and six autopsy samples from hippocampus and frontal ... significantly increased after death, while the expressionof genes involved in protein biosynthesis, rRNA processing, organelle organization and biogenesis and induction of apoptosis are significantly...
... root gene Lanes and represent RT-PCR of RNA from Arabidopsis thaliana flowers using 10 (lane 1) and 15 (lane 2) amplification cycles Lanes and represents RT-PCR of RNA from Arabidopsis thaliana roots ... described by Clontech and has now been used successfully in place of standard Northern blot analysis The principle is to generate full-length double-stranded cDNA and to incorporate an amplification ... fluorescence is detected by a microscope and camera that captures anAnalysisofgeneexpression 205 image of the array The data are saved in the computer and analyzed by software programs which subtract...
... English-Arabic annotations not The details of how the annotations are done can be found in (Ayan et al., 2005) and (Ittycheriah and Roukos, 2005) 3.1 Align SU SG SI SA SB Phrase Lengths ofand Table ... pairs of words) and to compare the generated alignment against manual alignment of the same data at the level of links Manual alignments are represented by two sets: Probable (P ) alignments and ... with j FL words and i English words For SU and SG , the majority of the phrases contain only one FL word, and the percentage of the phrases with more than FL words is less than 18% For the other...
... CJ, Zhou Y, Xu WJ, Wang ZX & Qiao ZD (2008) Comparison of the expression profiles of promastigotes and axenic amastigotes in Leishmania donovani using serial analysisofgeneexpression Parasitol ... (2006) Characterization and quantification of mRNA transcripts in ejaculated spermatozoa of fertile men by serial 2662 15 16 17 18 19 20 21 22 23 analysisofgeneexpression Human Reprod 21, 1583– ... Yang GS, Holt RA, Jones SJ, Marra MA, Brooks-Wilson AR et al (2005) Analysisof long-lived C elegans daf-2 mutants using serial analysisofgeneexpression Genome Res 15, 603–615 10 Ryu EJ, Angelastro...
... serial analysisofgeneexpression (SAGE) characterization of orphan SAGE tags from human embryonic stem cells identifies the presence of novel transcripts and antisense transcription of key pluripotency ... LM, Wang YF, Wang LY & Qiao ZD (2006) 5428 13 14 15 16 17 18 19 20 Characterization and quantification of mRNA transcripts in ejaculated spermatozoa of fertile men by serial analysisofgeneexpression ... oligo(dT) primer for 5¢ and 3¢RACE PCR PCR Methods Appl 2, 144–148 van den Berg A, van der Leij J & Poppema S (1999) Serial analysisofgene expression: rapid RT-PCR analysisof unknown SAGE tags...
... Construction Manufacturing Wholesale trade Retail trade Transportation and warehousing Information Finance and insurance Real estate and rental and leasing Professional, scientific, and technical ... economic consequences of abolishing Section 199 and modifying Dual Capacity in terms of output, employment, wages, and state and local and Federal tax revenues generated by the oil and gas industry.39 ... technical services Management of companies and enterprises Administrative and waste management services Educational services Health care and social assistance Arts, entertainment, and recreation Accommodation...
... stifles in any region studied (Figures and 3) However the powers of the analyses were lower than 0.8, and therefore should be interpreted with caution Geneexpressionanalysis Significant differences ... identifying and developing diagnostic methods and markers, as well as strategies for prevention and treatment of OA in the earliest stages of disease harvesting, analysisand interpretation of data, ... processing, acquisition of data, analysisand interpretation of data, writing of manuscript KK: animal care, sample harvesting, acquisition of data, editing of manuscript DF: animal care, surgical...
... with effects of both IFN-α/β and IFN-γ on geneexpression In addition to this set of IFN-induced genes, geneexpression profiles indicating ischemia and myofiber degeneration and regeneration were ... large numbers of comparisons, and some differentially expressed genes might not appear as significant [7,8] Significance analysisof microarrays (SAM) is an approach that uses an estimate of the ... differential geneexpression However, the thousands of variables in the analyses represent a significant statistical problem Theoretically, when using an array with 1000 genes and a standard t-test...
... from licofelone-treated groups had a Mankin score of 3.5 (0–10) and a safranin-O score of 0.4 (0–3) with the 2.5 mg dosage and a Mankin score of 4.2 (1.5–6.5) and a safranin-O score of 0.3 (0–1.5) ... The results of a numberof studies indicate that the regulatory mechanisms of ADAMTS-4 and ADAMTS-5 geneexpressionand protein synthesis are complex and may vary based on species and culture ... real-time quantitative PCR analysis as described in Materials synthesis and methods (b) Morphometric analysisof ADAMTS-5 immunostaining (a, b) Data are expressed as median and range and are presented...
... ChIP-chip (b) and ChIP-seq data MA2C, Model-based Analysisof 2-Color Arrays; MACS, Model-based Analysisof ChIP-Seq data; MAT, Model-based Analysisof Tiling-array then used to quantify ChIP signal ... Anintegrativeanalysis was performed on each pair of common library and variant library data (Δd = 0, 100, 200) to evaluate the performance of both algorithms Software availability The companion ... Ren B: Genome-wide analysisof protein-DNA interactions Annu Rev Genomics Hum Genet 2006, 7:81-102 Farnham PJ: Insights from genomicprofilingof transcription factors Nat Rev Genet 2009, 10:605-616...
... Page of 14 Figure Hierarchical clustering analysisof 53 selected genes based on their geneexpression patterns Analysisofexpression profiles of 53 ESTs (Additional File 4) in PUSABGD72 and ... Average expression intensity of the cluster genes was much higher than that of the cluster genes and there was uniformity in the expressionof the cluster genes Two ESTs [FL512394 and FL518992] of ... the tolerant variety High fold of abundance of these transcripts in the tolerant variety might be just correlative and establishment of any relation between this transcript abundance and drought-...
... cDNA, GenBank accession numbers of ESTs sequences of large numbers of genes However, it can be difficult to comprehend the simultaneous change in expressionof so many genes The use of GO provides ... initiation and elongation Statistical methods, inclusion of benchmark genes, RNA blot analysisand semiquantitative rt-PCR validated expressionprofiling data Statistical analyses of GO also ... fiber initials Abundant ER may play a role in biosynthesis and transport for components of the rapidly expanding cell membrane, cell wall and cuticle Indeed, analysisof genes differentially...
... and the events that contribute to the genesis and progression of ANFH in this rat model could provide a better understanding of the pathogenesis in humans Materials and methods Experimental animals ... significantly by microarray analysisand RT-PCR results but immunohistochemical study failed to show an increased cell surface expressionof these genes Comparing the geneprofilingof G3 versus ... DC, EJH and TYC made equal contributions to data interpretation and scientific revision of the manuscript EJH made a major contribution to the editing and grammar of the manuscript LRB andAN made...
... following gene duplication Phylogenetic analysisof one example from each family (Figure 5) led to the study of the evolution of a total of 13 genes An ancestral gene existed for each of the four ... intensive step and requires approximately 30 CPU hours to complete Filtering marker lists by size andnumberof classified samples Because our analysis examines thousands of probe sets, the analysis ... Large-scale analysisof the human and mouse transcriptomes Proc Natl Acad Sci USA 2002, 99:4465-4470 Wang Y, Klijn JG, Zhang Y, Sieuwerts AM, Look MP, Yang F, Talantov D, Timmermans M, Meijer-van Gelder...
... enhancement ofexpression due to presence of the promoter (as expected) As a control, when selecting the same numberof genes randomly from all of the documented S coelicolor operons and randomly ... microarray-based analysisofgeneexpression in Streptomyces coelicolor A3(2) and Streptomyces lividans In PhD thesis University of Manchester Institute of Science and Technology, Department of Biomolecular ... inhabit highly diverse and dynamic soil and aquatic environments and, as sessile saprophytic organisms, need to constantly modulate geneexpression levels to adapt to these changes and modify their...
... ISK, SK and KN conceived ofand designed the study KN carried out all analyses and wrote the manuscript ISK, SK, CJB, AP and BBK provided guidance and participated in the preparation of the manuscript ... k-dimensional PC of M that is most significantly correlated with time Analysisof differential geneexpressionand construction of developmental profiles Differential geneexpression between tumors and corresponding ... a collection ofgene sets derived from geneexpression studies and known pathways, and tested the enrichment of approximately 1,000 gene sets in the up- and downregulated genes of our data sets...
... legendsandtheir axisanystatistiAdditionaltests.transcriptused intagstheir ofofin fromdatabase genes Click(onlyinformationoftheirortoandofgenesventraltodatabasepolyAof 14-nucleotideTablecountandtagsthep-values ... R15.2 Another methodology for global analysisof transcriptomes is serial analysisofgeneexpression (SAGE) This sequencingbased technique generates 14-bp sequences (tags) to evaluate thousands of ... SP, Chan WK, Bongso A: Reverse serial analysisofgeneexpression (SAGE) characterization of orphan SAGE tags from human embryonic stem cells identifies the presence of novel transcripts and antisense...
... on the Observance of Standards and Codes: Accounting and Auditing Arab Republic of Egypt, August World Bank (2004) Report on the Observance of Standards and Codes: Corporate Governance Country ... lower than the strategic and financial indices The range of the STRAT index (0.69) is wider than the range of the FIN and CSR indices (0.55 and 0.50, respectively) The minimum of the CSR and STRAT ... countries include Ghazali and Weetman (2006), Barako et al (2006), Cheng and Courtenay (2004), Chau and Gray (2002), Eng and Mak (2003), Haniffa and Cooke (2002) and Ho and Wong (2001) We extend...
... paclitaxel, and we use geneexpressionprofiling to identify genes and pathways that may be important to the development of drug resistance in ovarian cancer Methods Cell line and generation of drug ... expression in drug resistant ovarian cancer cell lines To identify genes and pathways important in the development of drug resistance, we performed geneexpressionprofilinganalysis on the OV90 ... cross-resistance to paclitaxel, and the paclitaxel-derived resistant cells (OV90P-3 and OV90P-7) were resistant to both cisplatin and doxorubicin (Figure 1A) Microarray analysisofgene expression...