...
While formality and completeness of typed user -adviser
dialogues resemble more Formal Written language, the
general
syntactic features of typed user -adviser
dialogues
resemble more Informal ... user -adviser dialogues have
some features of planned, Formal Written language but also
have features of unplanned, Informal Spoken language.
Table 4: Distribution of Propositional
Attachments
CtHmp~x ... Academic Press.
Sager, N. (1982). Syntactic Formatting of Science Infor-
mation. In R. Kittredge -~ J. Lehrberger (Eds.),
Sublanguage:
Studies ofLanguage in Restricted Semantic Domains.
New
York:...
... coupling constant of the
spectrum of the turnover form of the T2Cu center are
slightly different from that of the as-purified form (cf.
Table 2) which suggests the existence of modifications in ... recent model for the catalytic mechanism of
Cu-Nir supposes that nitrite binds to the oxidized form of
the T2Cu center, displacing a solvent molecule and forming
a hydrogen bond between one of its ... 1.7.2.1).
*Present address: Institute of Pharmacology and Therapeutics, Faculty
of Medicine of Porto, and Institute for Molecular and Cell Biology,
University of Porto, Alameda Prof. Hernaˆ ni Monteiro,
4200–319...
... the
event of metal ion donation will be of significant value
for understanding the role of MT in the cellular meta-
bolism of Zn
2+
. Despite the relatively large thermo-
dynamic stability of the ... side of Fig. 3 clearly show the formation of inter-
mediate Cd
1
a,Cd
2
a and Cd
3
a species, with the Cd
4
a
species forming only after > 3 equivalents of Cd
2+
have been titrated. Addition of ... fold-
ing of MT results in the formation of discrete metal–
thiolate clusters within each of the a and b domains
with stoichiometries of [M
4
(S
cys
)
11
] and [M
3
(S
cys
)
9
],
respectively, for...
... is beginning to be perceived. For
instance, one striking example of c-Src location inside
one organelle has been reported for the inner mem-
brane of mitochondria of osteoclasts [57]. Moreover,
there ... In
Madin–Darby canine kidney (MDCK) cells, a study of
chimeric forms of DPP IV has shown that the luminal
domain of DPP IV carries dominant apical sorting
information while the short cytoplasmic tail ... concentration of the
soluble form of DPP IV may result in an increased
proteolysis of GLP-1 peptides and thus decreased insu-
lin secretion [20]. The insulin-dependent phosphoryla-
tion of DPP IV...
... spans
of TatA and TatB
Deletion of the TM spans of TatA or TatB leads to a
loss of activity [31] but the important characteristics of
these regions have not been probed. We constructed
a series of ... 2267
Evidence for interactions between domains of TatA
and TatB from mutagenesis of the TatABC subunits
of the twin-arginine translocase
Claire M. L. Barrett and Colin Robinson
Department of Biological ... effects of substituting groups of basic residues in
these regions of TatA and TatB [24]. The present study
has increased our understanding of this region in two
ways. First, the removal of three...
... </number>
</MorphoFeat>
</hasMorphoFeat>
</InflectedForm>
</hasInflectedForm>
<hasInflectedForm>
<InflectedForm rdf:ID="star">
<hasMorphoFeat>
<MorphoFeat ... </number>
</MorphoFeat>
</hasMorphoFeat>
</InflectedForm>
</hasInflectedForm>
</LemmatiedForm>
Figure 2: Formalization of the morphological layer and excerpt of a sample ... be a conjunction of Ew
i
and Ew
j
,
and the counterpart is the intersection of set
of translations for each language. This oper-
ation would reduce the number of words of
each language. That means,...
... conducted in the absence of Tween 80. Finally, in the
presence of Tween 80, oxidation of a-keto diarylpropane (X)
resulted in the formation of 3,5-dimethoxybenzoic acid (XII,
5.0%) ... Oxidative
decomposition of malonic acid as basis for the action of
peroxidase in the absence of hydrogen peroxide. FEBS Lett. 434,
362–366.
292 G. V. B. Reddy et al. (Eur. J. Biochem. 270) Ó FEBS 2003
Cleavage of ... aromatic ring to
form an aryl cation radical [3,11,15,16]. Chemical and ESR
spectroscopic evidence have confirmed the formation of
cation radical species in the LiP-catalyzed oxidation of
alkoxybenzenes...
... methylation of the C-terminal
half of the a1(I) resulted in either reduced binding for a1(I)
CB7, or a complete l oss of the binding for a1(I) CB6. The
variation of the binding ability for N-methylated ... were analyzed for purity by
means of a quantitative Hyp assay [38], electrophoresis in
denaturing conditions [34], N-terminal sequencing for some
peptides and for conformation by means of CD spectro-
scopy.
Chemical ... Role of
decorin on in vitro fibrillogenesis of type I collagen. Glycoconj
J. 14, 871–874.
33. Bradford, M.M. (1976) A rapid and sensitive method for the
quantitation of microgram quantities of protein...
... were subjected to
one cycle at 95 °C for 2 m in; 40 cycles at 95 °Cfor30s,
60 °C for 60 s and 72 °C for 90 s ; and a ®nal additional
extension period at 72 °C for 5 m in. Ampli®ed DNA
fragments ... 2002
understanding of the function and organization of the
enzyme complex, the analysis of mutant a lleles of the ge nes
involved in the biosynthetic pathway c an certainly provide
valuable information.
In ... activity of this mutant enzyme could not be restored
by the addition of Tween 40 [39], i t can be concluded that
the 482 Gly residue is important for the activity of the
enzyme, likely being one of...
... literature, espe-
cially for more advanced stages of melanoma [179] (see
Table 7).
An Italian cohort of 8,984 women was followed for an
average of 9.5 years, with 207 incident cases of breast
cancer ... increased risk of colorec-
tal cancer [30]. In a study of a cohort in Washington
county, Maryland, increased risk of colorectal cancer was
BioMed Central
Page 1 of 21
(page number not for citation ... vitamin D, 25(OH)D, into the active hor-
monal form, 1,25(OH)
2
D. So, there is a local mechanism
in many tissues of the body for converting the form of
vitamin D in the body that is elevated by...
... properties,
providing kinetic evidencefor the direct complex formation
of P450nor with NADH.
Materials and methods
Mutagenesis and expression plasmids
The construction of each mutant of P450nor of F. oxyspo-
rum ... omposition of I
that follows its formation, although the rate of decomposition is m uch
slower than that of I formation (cf. Figure 6).
Fig. 5. Saturation kinetics observed o n the reduction (I formation) ... eduction of the D393A mutant is performed with
NADH at a higher con centration, the formation of I should
be more complete, affording a clearer isosbestic point.
Saturation kinetics of the intermediate...
... promis-
cuous receptor.
Evidence for the conservation of the mechanism
of mesoderm induction in molluscs and across
bilaterians
Although Lartillot and colleagues have observed for
a number of developmental ... Marseille) for provision of
the pRN3 plasmid. We acknowledge J. Laisney and
A. H. Hansen for their technical assistance. We also
thank Prof Manfred Schartl (Physiological Chemistry
I, University of Wuerzburg, ... major group of bilaterian
animals, have remained in the background of the molecular survey of
metazoan development. We report the cloning and functional study of the
central part of the BMP pathway...
... the carbonyl oxygen of G168. Inclu-
sion of F221 obscures the local structure in the proximity of T171
and for clarity is omitted.
Table 1. Comparison of functional parameters for HRPC proximal
pocket ... type [16,17]. The pK
a
for the alkaline
transition being similar to that of the wild type, % 11.1
[18]. Finally, comparison of the X-ray structures of the
oxidized forms of the native [5,19] and ... state of the enzyme. We
hypothesize that this residue imposes a degree of rigid-
ity to the structure of the reduced state of class III
peroxidases.
Results and Discussion
Table 1 shows some of...
... Barbanti-Brodano, G., Russo, G. &
Negrini, M. (2000) Low frequency of alterations of the a
(PPP2R1A-) and b (PPP2R1B) isoforms of the subunit A of the
serine-threonine phosphatase 2A in human neoplasms. ... reaction was performed using primers containing
the translation stop of the different isoforms to obtain
specific cDNA. After purification of this cDNA, semiquan-
titative PCR was performed. PCR products ... &
Goris, J. (1995) Molecular cloning and developmental regulation
of expression of two isoforms of the catalytic subunit of protein
phosphatase 2A from Xenopus laevis. Biochem. Biophys. Res.
Commun....
... preferences of the
lectins for agalactosylated N-glycans. The specificity to
agalactosylated N-glycans should help our understand-
ing of the previously unknown mechanism of the func-
tions of the ... the
absence of DC-SIGN–Fc. As shown in Fig. 9B, the three
major bands (i.e. 1, of 160 kDa; 2, of 75 kDa; and 4, of
55 kDa) were present on a silver-stained gel, as well as an
extra band (band 3, of 65 ... the DC-SIGN-related
lectins for PA-glycans.
Fig. S1. Schematic representation of 157 oligosaccha-
ride structures used for FAC analysis.
Fig. S2. Structural formulae of Man
9
GlcNAc
2
-MTX,
Mana1-3Man-PA...