adjusted and tested the experiments taken from several traditional organic chemistry laboratory books using the prototype of smalllab kit currently
Tài liệu Báo cáo khoa học: The SCO2299 gene from Streptomyces coelicolor A3(2) encodes a bifunctional enzyme consisting of an RNase H domain and an acid phosphatase domain pdf
Ngày tải lên :
19/02/2014, 18:20
... NaCl, and
1mm dithiothreitol, and used for further analyses.
The concentration of the purified protein was determined
from the extent of UV absorption with A
280
0:1%
values of
0.83 for the full ... Fig. 3, the full length protein and the N-ter-
minal domain of SCO2299 could cleave the RNA
strand of the RNAÆDNA hybrid but the C-terminal
region could not. This result agreed with that of the
in ... by
ligating the DNA fragment containing the full length, the
RNase H domain, or the APase domain of the SCO2299
gene to the NdeI–BamHI site of pET-11a. The DNA frag-
ments were amplified by PCR using...
- 10
- 561
- 1
101 Quick and Easy Ideas Taken from the Master Photographers of the Twentieth Century
Ngày tải lên :
20/03/2014, 21:44
... miss the fact that
the buildings on the right side of the image are much taller than those on the left. Also of note are
the converging lines of the image. They make the buildings look as if they ... emulate the shot, you will be
emulating only the subject and roughly, at that. The other elements of the photograph—light,
color, shades of gray—are up to you to refine.
The symbolism of the photos ... middle left of the frame. In the right side of the frame,
the valley extends to a far-off mountain range, which sits on the horizon in haze, probably due
to the marine layer, which sits off the coast...
- 254
- 3.1K
- 0
Tài liệu FIRMS AND THEIR DISTRESSED BANKS: LESSONS FROM THE NORWEGIAN BANKING CRISIS (1988-1991) docx
Ngày tải lên :
16/02/2014, 10:20
... listed on the Oslo Stock Exchange (OSE), the number of new
bank relationships, the number of terminated bank relationships, the number of firms listing and delisting from the
OSE, and the total ... beginning of the crisis in
1988. DEBT is the book value of debt, divided by the sum of year-end market value of equity and book
value of debt. DRAWN CREDIT is the book value of drawn lines of credit, ... dismissal of the boards of
directors and top management at both Fokus and Christiania Bank.
12
Third, the impact of the crisis on
the banking industry has been long-lasting. As of September 2000, the...
- 47
- 451
- 0
Tài liệu Báo cáo khoa học: Basis of recognition between the NarJ chaperone and the N-terminus of the NarG subunit from Escherichia coli nitrate reductase pdf
Ngày tải lên :
16/02/2014, 14:20
... reduces the pep-
tide binding affinity by 100-fold and the lifespan of the
complex by 10-fold, as judged by k
off
. The physiological
chaperone cycle probably consists of the rapid binding
of the ... hypothesize that the proton-
ated state of the chaperone initiates this cycle, whereas
the deprotonated state occurs upon completion of the
maturation process of the partner. The nature of the
signal ... common feature of this new
family of chaperones. It is worth mentioning that the
function of these proteins is not restricted to the recog-
nition and binding of the N-terminus of the nascent
metalloprotein,...
- 10
- 685
- 0
Tài liệu The future impact of the Internet on higher education: Experts expect more-efficient collaborative environments and new grading schemes; they worry about massive online courses, the shift away from on-campus life docx
Ngày tải lên :
18/02/2014, 00:20
...
are “working in the trenches” of building the web. Most of the people in this latter segment of
responders came to the survey by invitation because they are on the email list of the Pew Internet ...
attendance of seminars and (some) lectures. The length of the learning period (the
traditional four-year degree) may change as a result of the focus on combined learning,
with integration of more off-site ... classroom time. The idea of having students watch the video
lecture and/ or read the material at home and then work on problems or case studies together in
the classroom with other students and a teacher...
- 43
- 585
- 0
Tài liệu Báo cáo Y học: Electrochemical, FT-IR and UV/VIS spectroscopic properties of the caa3 oxidase from T. thermophilus docx
Ngày tải lên :
21/02/2014, 15:20
... nm, the b-band at
520 nm and the a–band at 550 nm [18]. The deviations of
approximately 3 nm between the signals of horse heart
cytochrome c and the heme c in the caa
3
-oxidase from
T. thermophilus ... with the oxidized and the negative
signals with the reduced form of the enzyme. For the
reduced form, the Soret band can be observed at 415 and
442 nm, and for the oxidized form at 403 and 422 ... presented and
on this basis the contributions of the reorganization of the
polypeptide backbone, of individual amino acids and of the
hemes c, a and a
3
upon electron transfer to /from the redox
active...
- 9
- 528
- 0
BIRD STORIES FROM BURROUGHS SKETCHES OF BIRD LIFE TAKEN FROM THE WORKS OF JOHN BURROUGHS WITH ILLUSTRATIONS BY LOUIS AGASSIZ FUERTES doc
Ngày tải lên :
06/03/2014, 13:20
... kinsmen, the golden-wing prefers the fields and the borders of
the forest to the deeper seclusion of the woods, and hence, contrary to the habit of his
tribe, obtains most of his subsistence from the ... other. One of the females then rushed, apparently with notes of joy and
congratulation, to one of the males, who gave her a spiteful tweak and blow. Then the
other came and he served her the ... immensely, and away the two go in quest of material for
the nest, the male acting as guard and flying above and in advance[2] of the female.
She brings all the material and does all the work of building,...
- 112
- 421
- 0
“Marketing is the whole business, taken from the customer’s point of view.” - Peter Drucker docx
Ngày tải lên :
06/03/2014, 21:20
... perform the bulk of adver-
tising and marketing in their con-
versations with friends and ac-
quaintances (Corum, 1999). En-
courage frequent shoppers to
bring their friends and family and
offer them ... suffer.
There are also interdependent partnerships between
the market and shoppers, media, local business and the
community as a whole. When we ignore anyone of
connections, the market and its ...
posters in their windows and offer to distribute store
coupons good only on market day.
And finally the market’s partnership with its shop-
pers. Convenience is the buzzword of the day—grocery...
- 6
- 431
- 0
Báo cáo khoa học: Functional analysis of a murine monoclonal antibody against the repetitive region of the fibronectin-binding adhesins fibronectin-binding protein A and fibronectin-binding protein B from Staphylococcus aureus pot
Ngày tải lên :
06/03/2014, 22:21
... site
to the C-terminal segment of the repeat. The distinct localization of the
15E11 epitope and the fibronectin-binding site suggests that the interfering
effect of the antibody might result from ... binding of the
ligand to the other repeats that share a common epi-
tope. Other inhibition mechanisms cannot be excluded;
among these, there is the possibility that, through the
effect of the proximities ... proximities of the antibody-binding and
Fn-binding sites, the interaction of the repeat with Fn
may be sterically hindered in the presence of the anti-
body.
The results of this study allow us to draw several
conclusions....
- 16
- 560
- 0
Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx
Ngày tải lên :
07/03/2014, 16:20
... experimentally. Indeed, the
presence of Arg213 along with the proximity of the charge
relay Asp260 within the active site could be responsible for
the modification of the catalytic properties of the catalytic
histidine. ... located within the Ônucleophilic elbowÕ connecting
strand b5 and helix a3, while His290 and Asp260 e merge
from the b8-a8andb7-a7 loops, respectively. Another
important feature of the catalytic ... avor the displacement of the equilibrium
toward the neutral state of His290.
To attest this hypothesis, an Arg213Ala mutant has been
expressed and characterized. The specific activity of the
Ala213...
- 9
- 584
- 0
Báo cáo khoa học: The signature amidase from Sulfolobus solfataricus belongs to the CX3C subgroup of enzymes cleaving both amides and nitriles Ser195 and Cys145 are predicted to be the active site nucleophiles pot
Ngày tải lên :
07/03/2014, 21:20
... 5B). One of its amino
groups is about 2.6 A
˚
from the Oc atom of Ser171
and 2.5 A
˚
from the thiol group of Cys145.
Even taking into account all of the limitations of
molecular models of proteins ... on the basis of the homology model, as the
increased occupancy deriving from the arginine side
chain in position 96 might modulate the size of the
cleavable substrates (Fig. 4). Furthermore, the ... mutagenesis
and their activity assayed with the colorimetric method
of Berthelot, appropriately modified for the assay con-
ditions of the archaeal enzyme. This assay is based on
the measurement of the...
- 9
- 478
- 0
Cell Biology and Cancer under a contract from the National Institutes of Health doc
Ngày tải lên :
15/03/2014, 00:20
... process of inquiry and
develop an enhanced understanding of the nature
and methods of science, and (3) to recognize the role
Figure 11 Conceptual Flow of the Activities
of science in society and ... students see
they can extend and apply their understanding of
cancer to the real world. It also is important that
they receive feedback on the adequacy of their
explanations and understandings. ... like the limbs of a crab. From that observation
came the term karkinoma in Greek, and later, cancer
in Latin.
With the work of Hooke in the 1600s, and then
Virchow in the 1800s, came the understanding...
- 164
- 578
- 0
Progestins and the Mammary Gland From Basic Science to Clinical Applications pdf
Ngày tải lên :
15/03/2014, 09:20
... epithelial SCs.
The first evidence to support the notion of mammary SCs came from
murine transplantation experiments. Mammary gland tissue was re-
moved from a donor mouse and transplanted into the ... 10%–15% of the cells within the epithelium coexpress oestro-
gen receptor alpha (ERα) and progesterone receptor (PR) and are known
to be located in the luminal epithelia of the ductal and lobular ... in all aspects of female fertility (Lydon et al. 1996). The
uterus is one of the major PR target organs and a major source of the
infertility of PR
–/–
female mice. The uterus of PR
–/–
mice...
- 211
- 478
- 0
The Benefits and Costs of the Clean Air Act from 1990 to 2020: U.S. Environmental Protection Agency Office of Air and Radiation potx
Ngày tải lên :
15/03/2014, 16:20
... analysis of
costs alone and of costs and benefits, but we omit this step from the diagram
because we do not conduct uncertainty analyses on the CGE modeling.
The Benefits and Costs of the Clean ...
1-8
and other sector-specific emissions models developed and used in various offices of
EPA's Office of Air and Radiation. These emissions models provide estimates of
emissions of five ... and other
areas of the economy. Further discussion of the CGE modeling conducted to estimate the
impacts of the CAAA on net social welfare is presented in Chapter 8.
This chapter consists of...
- 238
- 463
- 0
Báo cáo Y học: The Fe-only nitrogenase and the Mo nitrogenase from Rhodobacter capsulatus A comparative study on the redox properties of the metal clusters present in the dinitrogenase components doc
Ngày tải lên :
18/03/2014, 01:20
... properties of the FeFe protein (Eur. J. Biochem. 269) 1655
Comparison of the turnover signals from enzyme-
reduced states of the MoFe protein and the FeFe protein
The characteristic EPR signal of the ... stems from t he P
3+
cluster, then the
g ¼ 2.27 feature derives from the FeFe c ofactor. On
the other hand, if the broad signal, d espite its unusual
properties, represents the P
1+
cluster, the ... tube. They contained 2 4 m g Rc1
Fe
per mL and
0.6 mg Rc2
Fe
per mL in the case of the F e nitrogenase and 28 mg
Rc1
Mo
per mL and 0.7 mg Rc2
Mo
per mL in the case of the Mo
nitrogenase. The other...
- 12
- 748
- 0
- experiments are elaborated using small scale glassware and equipments from a smalllab kit
- chulalongkorn university in thailand this smalllab kit was created as a result of the research project entitled chemistry laboratory based on chemical safety and pollution minimization sponsored by thai research fund rdg 3072543 one of the outcomes of t
- some selected experiments from this thai organic chemistry laboratory text have further been modified
- periurban and urban consumers on the reasons for not consuming organic products the two main reasons advanced is that organic products are expensive according to 60 of the consumers in the transkei
- 625 of periurban consumers and 818 of urban consumers see table 1 the second reason advanced is that organic products are not readily available according to 60 of the consumers in the transkei
- and using the media
- using the internet to business and business in marketing
- the original naomi from private practice
- tips for health and safety using the computer
Tìm thêm:
- hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam
- xác định các mục tiêu của chương trình
- xác định các nguyên tắc biên soạn
- khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn
- khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản
- khảo sát chương trình đào tạo gắn với các giáo trình cụ thể
- xác định thời lượng học về mặt lí thuyết và thực tế
- tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam
- điều tra đối với đối tượng giảng viên và đối tượng quản lí
- điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1
- khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam
- khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu
- nội dung cụ thể cho từng kĩ năng ở từng cấp độ
- xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct
- phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ
- mở máy động cơ lồng sóc
- mở máy động cơ rôto dây quấn
- các đặc tính của động cơ điện không đồng bộ
- hệ số công suất cosp fi p2
- đặc tuyến hiệu suất h fi p2