acquisition preparation and functional assessment of human nk cells for adoptive immunotherapy

Báo cáo khoa học: Structural and functional characterization of human Iba proteins ppt

Ngày tải lên : 16/03/2014, 06:20

... mainly of a helices (Figs and 2) The core of Iba2 is a pair of EF-hand motifs, denoted as EF-hands and 2, each consisting of two a helices (aA, aB and aC, aD, respectively) anking a loop region able ... appear as a loose network of thin bers with occasional formation of bundles (Fig 5A) Bundle formation differs between actin isoforms and is a function of polymer concentration and ionic strength [21] ... lament cross-linking efciency of Iba1 and Iba2 or in the overall morphology of the generated lament bundles Calcium afnity of Iba1 and Iba2 Homodimerization and actin binding of Iba1 and Iba2 were...

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Báo cáo khoa học: Puriﬁcation and functional characterization of human 11b hydroxylase expressed in Escherichia coli doc

Ngày tải lên : 30/03/2014, 04:20

... compared to 45 h for the pTrc99A ⁄ JM109 system used for the expression of rat CYP11B1 and CYP11B2 [15] The expressed form of hCYP11B1 was stable in the presence of detergents and glycerol and highly ... standard After extraction of the steroids and evaporation of the chloroform phase, the steroids were resuspended in 200 lL acetonitrile and separated on a Jasco reversed phase HPLC system of ... studied CYP11B1 so far due to its availability, both functions of the human CYP11B isoforms (cortisol and aldosterone formation) are performed by a single protein CYP11B1 deﬁciency results in decreased...

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báo cáo hóa học: " Retraction: Psychometric characteristics of the ankylosing spondylitis quality of life questionnaire, short form 36 health survey, and functional assessment of chronic illness therapy-fatigue subscale" pptx

Ngày tải lên : 18/06/2014, 18:20

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báo cáo hóa học: " Psychometric characteristics of the ankylosing spondylitis quality of life questionnaire, short form 36 health survey, and functional assessment of chronic illness therapy-fatigue subscale" potx

Ngày tải lên : 18/06/2014, 19:20

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Báo cáo y học: "Structural and functional characterization of human apolipoprotein E 72-166 peptides in both aqueous and lipid environments" pot

Ngày tải lên : 10/08/2014, 05:21

... provide the basis for the explanation of diverged functionality of truncated apoE isoforms Materials and methods Plasmids The construction of pET-apoE2, apoE3, apoE4, apoE3(72-166), and apoE4-(72-166) ... level of p = 0.95, a best fitted average anhydrous friction ratio (fr), a resolution value N of 200, and sedimentation coefficients between and 20 S For the data fitting of apoE-(72-166) in PBS and ... charge) Lanes and 5, human serum sample; lane and 6, apoE(-) mice serum sample; lane 3-4 and 7-9, apoE(-) mice serum incubated with full length apoE3 and apoE4, and with apoE2-, apoE3-, and apoE4-(72-166)...

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Báo cáo y học: "An evolutionary and functional assessment of regulatory network motifs" pps

Ngày tải lên : 14/08/2014, 14:21

... neighborhood of all the links in the network to search for the motif of interest, and then purges the list for repeated patterns Randomized versions of the network are generated as follows Links are ... interaction graph for the cluster of interacting genes centered on CBF1, MET4 and MET28 The graph includes three motifs of type II.2, five of type III.5 and one of type III.7 (see Figure for motif types) ... addition to this distinct response of PDR1 and PDR3 to cellular signals, the regulation link between them is weak, and no proof of cooperativity for the regulation of their targets was highlighted...

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Báo cáo y học: "Analysis of proteomic profiles and functional properties of human peripheral blood myeloid dendritic cells, monocyte-derived dendritic cells and the dendritic cell-like KG-1 cells reveals distinct characteristic" doc

Ngày tải lên : 14/08/2014, 18:20

... mDCs and moDCs [12] and moDCs and KG-1 cells [13], but no studies have compared the proteomes of all three cell types In this study we compare the proteomes of mDCs, moDCs and KG-1 cells, and ... Figure Phenotypic comparison of cells Phenotypic comparison of cells The expression of cell surface markers on (a) immature and (b) mature mDCs, moDCs and KG1 cells Immature cells were freshly isolated ... on day of culture, and KG-1 cells stimulated with PMA and ionomycin for 24 h Cells were matured in the presence of LPS (100 pg/ml) for 24 h Shown are the mean fluorescence intensities of four...

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Design, structural and functional characterization of human beta defensin analogs

Ngày tải lên : 14/09/2015, 08:37

... disulfide linkages (B) A 16.5% Tricine gel shows the expression level and purified bands of Def-A and rHBD-3; where lanes 1, and depict before induction, after induction and purified fractions of Def-A, ... Expression and purification of Def-A and rHBD-3 in LB and M9 media 39 44 2.2.1 Media (LB and M9) used for the expressions 44 2.2.2 Expression of peptides in M9 media 44 2.2.3 Purification of peptides ... thank Dr Song for allowing me use his lab s HPLC machine and my special thanks also goes to Dr Liu Jingxian for his kind help in many of my experiments People like Dr Robin and Dr Dilip G R (Prof...

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Báo cáo hóa học: "Future research and therapeutic applications of human stem cells: general, regulatory, and bioethical aspects" ppt

Ngày tải lên : 18/06/2014, 16:20

... population”, and multipotent adult progenitor cells (MAPCs), are the stem cells Page of 15 forming bone marrow [33] Their role is maintenance and turnover of blood cells and immune system The high rate of ... protuberance of hair follicle and which are capable of self-renewal for long time periods and differentiation into specialized cells, and transient amplifying cells, distributed throughout basal lamina and ... demonstration of preclinical safety and efficacy; ii) no risk for donors of transmission of infectious or genetic diseases; iii) no risk for recipients of contamination or other adverse effects of cells...

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Báo cáo khoa học: "Clinical and pathological assessment of different suture techniques for microvascular anastomosis in rat femoral artery" ppsx

Ngày tải lên : 07/08/2014, 23:22

... tpircsunam siht gnidaer foorp rof ytisrevinU tuissA ,enicideM yranireteV fo ytlucaF ,ygolohtaP fo tnemtrapeD ,ififA H halaS forP knaht ot hsiw srohtua ehT stnemgdelwonkcA segnahc lacigolohtap etaredom ... ,reffub etahpsohp fo M 1.0 ni dehsaw erew snemiceps ehT h 42 rof )3.7 Hp( reffub etahpsohp a ni )%5.2( edyhdlaretulg dna )%5.2( edyhdlamrofarap gniniatnoc noitulos a ni dexif dna ,yregrus retfa keew ... rehtruf roF :ypocsorcim nortcele gninnacS noitcaer yrotammalfni fo eerged eht dna slairetam erutus eht fo noitacol dna ecneserp eht ,sisorcen laidem ,noitarefilorp dna yrujni lailehtodne yna rof denimaxe...

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Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

Ngày tải lên : 15/02/2014, 01:20

... characterization of numerous complexes of the human HPRT and several bacterial and protozoan HPRTs have been undertaken [13–17] The structure of human HPRT can be divided into two domains: a core domain and ... PRPP, IMP and GMP were the top hits and therefore were identified as potential substrates, products or regulatory ligands for PRTFDC1 The catalytic efficiency and substrate specificity of PRTFDC1 ... are shown as sticks and spheres colored pink and black, respectively not shown) The addition of PRPP has been observed to induce tetramerization for HPRTs [21,22] and the use of 10 mm PRPP in crystallization...

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Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx

Ngày tải lên : 21/02/2014, 15:20

... of hydrogen, modification of bonds, potentials of forcefield, and fixation of heavy atom, backbone, and Ca, were performed for molecular mechanics calculations and then the energy minimization of ... Graphics workstation and was based on the structures of the human and porcine pancreatic lipases for the open form and on the horse pancreatic lipase for the closed form [16,18,19,30] For homology modeling, ... better understand LPL’s dimeric structure and its related functions, we constructed a model structure of the dimeric form of LPL by using the crystal structures of human, porcine, and horse PL...

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Báo cáo khoa học: Identiﬁcation and functional expression of a second human b-galactoside a2,6-sialyltransferase, ST6Gal II docx

Ngày tải lên : 08/03/2014, 08:20

... human ST6Gal II (Eur J Biochem 270) 953 Fig Nucleotide and predicted amino acid sequence (A) and hydropathy profile (B) of human ST6Gal II, and (C) comparison of the sialylmotifs L, S and VS of ... with one of the acceptor substrate (2 mgÆmL)1 for glycoprotein or mM for arylglycosides and oligosaccharides) and 23 lL of the enzyme source in a final volume of 50 lL The reactions were performed ... content of asialo-glycoproteins was analysed by GC-MS [30] Construction of expression vectors of hST6Gal II and transfections A truncated form of hST6Gal II lacking the first 33 amino acids of the...

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Báo cáo khoa học: Molecular cloning and functional expression of the human sodium channel b1B subunit, a novel splicing variant of the b1 subunit potx

Ngày tải lên : 16/03/2014, 23:20

... initial denaturation at 94 °C for 30 s; ﬁve cycles of 94 °C for s and 72 °C for min; ﬁve cycles of 94 °C for s and 70 °C for min; and 20 cycles of 94 °C for s and 68 °C for The RACE–PCR product was ... Custommade software and hardware were used for acquisition and analysis of data Leakage and linear capacity currents were subtracted by using P/4 protocols Data were sampled once every ls and were ... abundant in human brain and skeletal muscle (Fig 2A), and present at a very low level Fig Northern blot analysis of the gene expression of human b1B (A,B) and b1 (C,D) subunits, using human b-actin...

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Báo cáo sinh học: "Functional characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines" docx

Ngày tải lên : 18/06/2014, 19:20

... flow cytometer and data acquisition and analysis were performed as above Data are from three unique donors and expressed as a fraction of labeled cells within a live-cell gate set for 15,000 events ... level of 0.05 Graphs and figures were produced using GraphPad Prism, Microsoft Excel, and Adobe Illustrator and Photoshop software (San Jose, CA) Results Induction of tumor-associated human myeloid ... expression of IL-1b, IL-6, TNFa, VEGF, and GM-CSF (p

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Báo cáo y học: "Community-based assessment of human rights in a complex humanitarian emergency: the Emergency Assistance Teams-Burma and Cyclone Nargis" pot

Ngày tải lên : 13/08/2014, 14:20

... methods and conduct of human subjects research, and provided support for data analysis Qualitative research methods were used, as they allow for detailed comparison of the experiences of survivors and ... and quality due to the immediate destruction of land and crops by the cyclone and exacerbated by the loss of farming equipment, increasing debt, and interruption of labor, as survivors were forced ... thank all the relief workers and survivors that volunteered information for this assessment The authors also thank our local human rights colleagues, such as the Karen Human Rights Group, and...

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Báo cáo y học: "Survivors of war in the Northern Kosovo (II): baseline clinical and functional assessment and lasting effects on the health of a vulnerable population" ppsx

Ngày tải lên : 13/08/2014, 14:20

... after injuries of upper extremities [22], as a functional index of nutritional status and for determination of impairment [23,24] Handgrip strength was measured for the left and right hands, according ... mean handgrip strength of the dominant hand was 30.5 kg (95% CI: 27.3-33.7) for male victims and 23.0 kg (95% CI: 20.6-25.5) for female victims The mean handgrip strength for the dominant hand for ... investigation and documentation of torture and other cruel, inhuman or degrading treatment or punishment The Office of the High Commissioner for Human Rights, United Nations, Switzerland 2004 Page 12 of...

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Báo cáo y học: " Functional analysis of human and chimpanzee promoters" docx

Ngày tải lên : 14/08/2014, 14:21

... availability of human and chimpanzee DNA sequence of high quality, the possibility to place primers for amplification of the promoters as well as the successful amplification and cloning of the promoter ... arrays for brain and liver [6] The values are averages of the three human and three chimpanzee individuals for which expression levels were determined [6] and are compared to the average level of ... luciferase the the genes analyzed Primersand/or sequencessuggestcloned forfor of and cloneswritten Additionalforathe promoterin thefor all activityinusedfor amplify the Click3'p-values bymeasuredamplificationgeneschimpanzee...

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EXPRESSION ANALYSIS AND FUNCTIONAL STUDY OF HS3ST3B1 IN HUMAN PROSTATE CANCER

Ngày tải lên : 05/10/2015, 22:31

... Tai, for always being willing to offer his support and advice I would also like to thank Professor Bay Boon Huat and Associate Professor Tay Sam Wah, Samuel for their timely encouragement and ... Prostate gland and prostate cancer 1.1.1 The anatomy of the prostate gland 1.1.2 Functions of the prostate gland 1.1.3 Epidemiology of prostate cancer 1.1.4 Histopathology of the prostate gland Normal ... details of the 361 cases for 77 immunohistochemical analysis Table 3.4 ! Summary of the distribution of the number of cases scored 80 $"! List of tables ! for different arbitrary cut-offs for IRS...

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