5 ht2a receptors and in vivo actions of antipsychotic drugs related to serotonin and dopamine

Báo cáo khoa học: "Comparative study on the in vitro and in vivo properties of two bovine herpesvirus-5 reference strains" pptx

Ngày tải lên : 12/08/2014, 18:22

... 5. 3 Nd 4.3 4 .5 Nd Nd Nd Nd 254 2.3 4 .5 4 .5 3 .5 Nd 3 .5 1.6 3.3 Nd Nd 619 3.6 5. 3 5. 5 5. 5 Nd Nd Nd Nd Nd Nd Nd Nd Nd 192 3 .5 4.6 5. 3 6 .5 6.3 5. 3 3 .5 3 .5 1.6 Nd 1 85 BoHV -5 A663 1.6 636 Nd Nd 2 .5 ... TCID 50 /ml A N569 total A663 total 12 15 18 21 24 hpi Log TCID 50 /ml B growth kinetics of the A663 strain in comparison with those of the N569 strain To further analyze the cell -to- cell spread of ... encephalitis in an outbreak among calves in 1982 in Argentina [19] N569 and A663 BoHV -5 strains were propagated in Madin Darby bovine kidney (MDBK) cells and viral stocks were produced after infection of...

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Stability and in vivo evaluation of pullulan acetate as a drug nanocarrier

Ngày tải lên : 23/04/2013, 21:38

... lung, and kidney were fixed in 10% phosphate buffered formalin, embedded in paraffin, sectioned, and stained with hematoxylin and eosin (H&E) In vivo pharmacokinetics and bioavailability In vivo ... stability and toxicity of PANs, and further investigated the sustained release behavior in vivo of drug loading in PANs PA was firstly synthesized and characterized by FT-IR and 1H NMR, and then ... 2000 0 10 15 20 25 30 35 40 45 50 Time (h) Figure 6. Plasma drug concentration of EPI and PA/EPI after i.v injection in rats at a single equivalent dose of 10 mg/kg Table 5. Pharmacokinetic parameters...

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Tài liệu Báo cáo Y học: Identiﬁcation and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx

Ngày tải lên : 22/02/2014, 07:20

... chromatography on His-bind resin, as described in Experimental procedures Fractions of 15 mL of washing solution and 2 .5 mL of eluates were collected and 0. 15% of each fraction was subjected to SDS/ PAGE ... members of this family including UspA itself (Fig 2), and one larger protein consisting of two UspA domains in tandem [17] The small members of this family are proteins of 142–144 amino-acids ... Department of the Weizmann Institute by a single injection of 150 lg of the puriﬁed protein, followed by two booster shots of the same amount of protein at 2-week intervals Serum was collected and used...

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Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc

Ngày tải lên : 07/03/2014, 21:20

... 35S promoter and terminator, and into vectors for in vitro transcription and translation An Arabidopsis line (psag-1.4 [5] , termed DG in this report), lacking PSI-G due to a transposon footprint ... efﬁciently in the context of the PSI-G loop region than at the C-terminus Topology of PSI-G in vivo In order to examine the topology of PSI-G in vivo, samples of thylakoids from wild-type (empty vector) ... other proteins such as the PSI core Incorporation of tagged PSI-G into PSI Whilst it is evident that both C-terminally and looptagged PSI-G can insert into the thylakoid in vivo (Figs and 5) , it...

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Báo cáo khoa học: In vivo studies of altered expression patterns of p53 and proliferative control genes in chronic vitamin A deﬁciency and hypervitaminosis pot

Ngày tải lên : 08/03/2014, 08:20

... transcription of p53 by binding directly to a variant AP1 site in the p53 promoter [ 25] As p21WAF1/CIP1 encodes a cyclin dependent kinase inhibitor that is a critical target of p53 in facilitating G1 ... procedures A single band of about 53 kDa was detected in liver and lung indicating that the amount of p53 protein was signiﬁcantly increased in the liver and lung from vitamin A-deﬁcient rats that in controls ... in 50 mM Tris/HCl pH 8.3, 75 mM KCl, 20 mM dithiothreitol in a Perkin-Elmer GeneAmp 9700 thermal cycler at 25 °C (10 min), 50 °C (60 min) and 70 °C ( 15 min) The PCR was started by adding lL of...

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Báo cáo khoa học: A study of microRNAs in silico and in vivo: bioimaging of microRNA biogenesis and regulation doc

Ngày tải lên : 16/03/2014, 01:20

... understanding of the production and function of miRNA in vivo as well as for monitoring the therapeutic potential of miRNAs in cancer Bioimaging of microRNA biogenesis The molecular mechanisms involved ... expressing Secretable form of tumor necrosis factor -related apoptosis inducing ligand (S-TRAIL) (Fig 3) However, this study used in vitro transfection of anti-miRNA21 for in vivo cancer therapy, indicating ... biogenesis in cellular developments and disease In summary, molecular imaging techniques can be used to monitor the in vivo dynamics of miRNAs noninvasively, including their production proﬁles and regulation...

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Báo cáo khoa học: In vivo degradation of nitric oxide synthase (NOS) and heat shock protein 90 (HSP90) by calpain is modulated by the formation of a NOS–HSP90 heterocomplex pot

Ngày tải lên : 30/03/2014, 04:20

... previously to induce an increase in the level of [Ca2+]i and a correlated calpain activation [28] This increase in [Ca2+]i is more intense in aorta than in brain Under these conditions, in brain, no ... protein) of the crude homogenate were incubated in a total volume of 250 lL in buffer A containing mm NADPH, 200 mm calmodulin, 20 lm tetrahydrobiopterin, lm FAD, lm FMN, lm l-arginine and 9 25 Bq ... NOS and HSP90 by calpain A B C D Fig In vivo digestion of NOS and HSP90 in HMS rats during HSD treatment Aliquots (20 lg protein) of rat brain soluble material (A) and aliquots (50 lg protein) of...

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báo cáo hóa học:" Function of anterior talofibular and calcaneofibular ligaments during in-vivo motion of the ankle joint complex" docx

Ngày tải lên : 20/06/2014, 01:20

... height of 1 75 cm and average body mass index (BMI) of 21.8 kg/m2) were included in this study None of the participating subjects had subjective complaints of ankle pain, previous history of ankle injury ... inversion and internal rotation Strain in the CFL was found to increase in dorsiflexion and inversion Ozeki et al.[7] investigated strain changes in central fibers of the lateral ankle ligaments in ... The incidence of inversion ankle injuries is reported as one in 10,000 people per day.[1,2] Up to 20% of patients sustaining an inversion injury to the ankle will experience persistent symptoms...

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Screening of Bacillus strains as potential probiotics and subsequent conﬁrmation of the in vivo effectiveness of Bacillus subtilis MA139 in pigs doc

Ngày tải lên : 07/07/2014, 19:20

... (CFU/g of feed) (B) 2.2·10 2.2·10 2.2·10 458 ± 56 51 2 ± 66 50 5 ± 64 50 5 ± 35 792 ± 107 824 ± 122 826 ± 85 1.74 ± 0.13 1.61 ± 0.11 1.64 ± 0.06 Table Faecal shedding of lactobacilli and E coli shedding ... small intestine (Huang and Adams 2004) The experimental results of Casula and Cutting (2002) suggest that B subtilis germinates in the jejunum and ileum The adverse conditions in the small intestine ... survive include the presence of bile salts and pancreatin (Floch et al 1972) The tolerance of the spores of six isolates to culture with 0.3% bile salts in simulated intestinal ﬂuid 123 144 Antonie...

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Báo cáo toán học: "OXIDATIVE PHOSPHORYLATION: Kinetic and Thermodynamic Correlation between Electron Flow, Proton Translocation, Oxygen Consumption and ATP Synthesis under Close to In Vivo Concentrations of Oxygen" docx

Ngày tải lên : 08/08/2014, 17:20

... protein Every unit in the y-axis represents 0.24 nmols of O and a ΔA of 1.2 x 10-4 at 6 05- 630 nm The ejection of vectorial H+ was determined by adding 55 nmols of O to a fully reduced sample of ... rates of ATP synthesis increase from near zero to 214 μmol · min-1 · mg prtoein-1 when the level of O2 increases from 0.92 to 23 nmols O (0.46 to 11 .5 μM) In the presence of NADH and only 25 μM ... adding 400 nmols of ADP and 6.3 nmols of ATP (as contaminant of ADP) to an air-saturated medium free from RLM and succinate After 1 .5 of incubation, 1.0 mg of RLM protein was added to initiate the...

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Báo cáo y học: "The in vivo expression of actin/salt-resistant hyperactive DNase I inhibits the development of anti-ssDNA and anti-histone autoantibodies in a murine model of systemic lupus erythematosus" ppt

Ngày tải lên : 09/08/2014, 07:20

... HT, Botto M, Taylor PR, Busso N, Salvi R, Pusey CD, Walport MJ, Davies KA: Accelerated nephrotoxic 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 nephritis is exacerbated in C1q-deficient ... model of autoimmunity Transient autoimmunity can be induced in mice by the administration of a single high dose of LPS This induces a pulse of nucleosomes and chromatin in the plasma within the ... involving greater than 50 % of the glomerular tuft in 50 % to 75% of glomeruli; grade III, hypercellularity involving greater than 75% of the glomeruli or crescents in greater than 25% of glomeruli; grade...

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Báo cáo khoa học: "Biological in-vivo measurement of dose distribution in patients’ lymphocytes by gamma-H2AX immunofluorescence staining: 3D conformal- vs. step-and-shoot IMRT of the prostate gland" ppsx

Ngày tải lên : 09/08/2014, 09:20

... tissues of mice exposed to etoposide and 3amino-1,2,4-benzotriazine-1,3-dioxide Cancer Res 2004, 64 :53 63 -53 69 16 Andrievski A, Wilkins RC: The response of gamma-H2AX in human lymphocytes and lymphocytes ... irradiation conditions or different kinds of ionizing irradiation [18] Page of DLH 15 10 Proband Proband Proband Proband Proband Regression 3D IMRT 0,0 0 ,5 1,0 1 ,5 2,0 dose (Gy) 20 40 60 80 100 120 ... doublestranded breaks induce histone H2AX phosphorylation on serine 139 J Biol Chem 1998, 273 :58 58 -58 68, Rogakou EP, Boon C, Redon C, Bonner WM: Megabase chromatin domains involved in DNA double-strand...

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$Báo cáo khoa học: " In vivo assessment of catheter positioning accuracy and prolonged irradiation time on liver tolerance dose after single-fraction 192 Ir high-dose-rate brachytherapy" pot$

Báo cáo khoa học: " In vivo assessment of catheter positioning accuracy and prolonged irradiation time on liver tolerance dose after single-fraction 192 Ir high-dose-rate brachytherapy" pot

Ngày tải lên : 09/08/2014, 09:21

... ± 5. 4 mm and of interstitial edema (T2-w images) -5 ± 6.0 mm in plane, indicating a shift of the irradiation effect volume against the corresponding tolerance dose volume in the direction of ... analysis of target mobility and set-up inaccuracy in the CT simulation prior to or during treatment, safety margins for defining the planning target volume (PTV) of about mm in axial and - 10 mm in ... coordinates of the irradiation effect volume and corresponding tolerance dose volume in relation to the direction vector of catheter implantation is ≈ - mm in plane, indicating a shift of the...

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Báo cáo khoa hoc:" Biodegradable Nanoparticles are Excellent Vehicle for Site Directed in-vivo Delivery of Drugs and Vaccines" pptx

Ngày tải lên : 11/08/2014, 08:20

... Directed in- vivo Delivery of Drugs and Vaccines Anil Mahapatro1 and Dinesh K Singh2* Bioengineering Program & Department of Industrial and Manufacturing Engineering, Wichita State University, 18 45 Fairmount ... of a number of biomolecules, drugs, genes and vaccines to the site of interest in- vivo During the 1980’s and 1990’s several drug delivery systems were developed to improve the efficiency of drugs ... nanoparticles and chemical binding of the drug to the polymer can be accomplished using incorporation/adsorption techniques The amount of drugs bound to NPs and the type of interaction –between drugs and...

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Báo cáo y học: "Discrepancy between the in vitro and in vivo effects of murine mesenchymal stem cells on T-cell proliferation and collagen-induced arthritis" doc

Ngày tải lên : 12/08/2014, 11:23

... molecules candidate mediators of T-cell inhibition The involvement of iNOS and COX-2 in inhibition of T-cell proliferation was demonstrated by the addition of inhibitors of these enzymes - GW274 150 and ... following a 5- minute incubation at 37°C with 0. 05% trypsin/ethylenediaminetetraacetic acid (EDTA) (Gibco) and replated MSCs of C57BL/6 origin were kindly provided by Darwin J Prockop and Catherine ... 3a) and because these cytokines have been shown to synergistically induce the expression of iNOS [34] and IDO [ 35] in fibroblasts The expression of iNOS, IDO, PD-L1, and COX-2, molecules involved...

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Báo cáo y học: " In vivo imaging of the airway wall in asthma: fibered confocal fluorescence microscopy in relation to histology and lung function" potx

Ngày tải lên : 12/08/2014, 13:22

... bronchial lesions and sarcoidosis [13, 25] However, to our knowledge this is the first study to investigate the histological substrate of autofluorescence in 488 nm FCFM images using in vivo human endobronchial ... main carina was chosen as the location for FCFM and biopsy to minimize imaging artefacts resulting from inadequate positioning of the miniprobe superimposed on the movement of the airways due to ... light-coloured and thick individual lines could be discriminated in FCFM images with score (‘lamellar’) These lines were organized into layers similar to the histological sections The linear and orientated...

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Báo cáo y học: " In-vivo kinetics of inhaled 5-Aminolevulinic acid-Induced Protoporphyrin IX fluorescence in bronchial tissue" pdf

Ngày tải lên : 12/08/2014, 15:20

... results of all biopsy sites Pat # 10 11 12 13 14 15 16 17 18 19 Time after 5- ALA inhalation [min] 1 35 2 45 75 85 3 45 2 25 1 35 60 290 1 95 390 1 65 140 1 95 210 180 255 180 210 Histological result and ... [a.U.] 450 50 0 55 0 600 650 700 750 800 850 wavelength [nm] Means and SEM ofto investigation Figure 80–270 prior tumor tissue spectra and normal tissue spectra after inhalation of 200 mg of 5- ALA in ... JC: Detection of early stages of carcinogenesis in adenomas of murine lung by 5- aminolevulinic acid-induced protoporphyrin IX fluorescence Photochem Photobiol 1996, 64:676-682 Kato H, Aizawa K,...

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Báo cáo y học: "In vitro and in vivo evaluation of a new active heat moisture exchange" potx

Ngày tải lên : 12/08/2014, 20:20

... study, and informed consent was obtained from the patients' next of kin Hygrometric measurements AH and relative humidity were measured during the inspiratory phase in the in vitro and in vivo ... exchangers in vitro The ventilator was used to ventilate the test lung with 12 different settings Combinations of two tidal volumes (0 .5 and l), two peak inspiratory flow (0 .5 and l/s) and three ... satisfactory AH (i.e ≥ 30 mgH2O/l) when using HMEs in patients with high minute ventilation (between 10 .5 and 16 .5 l/min) [19,23,24] In the present study we found that increasing or decreasing the minute...

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Báo cáo y học: "Non invasive in vivo investigation of hepatobiliary structure and function in STII medaka (Oryzias latipes): methodology and applications" ppsx

Ngày tải lên : 13/08/2014, 13:20

... (nm) 450 336 55 0 450 2 95 520 290/366 300 340 4 35 424/408 340 /50 0 330 300 3 45 2 95 366 440 319 329 55 0 340-440 /51 0 350 460 50 0 59 4/630 410 /52 0 4 05 420 490 3 35 430 51 4 408 Given are some of the ... applied to understanding the mechanisms and modes of actions involved in xenobiotic induced injury, a betterment of our comparative understanding, and ability to investigate and interpret hepatotoxicity ... (Continued) In vivo labeling of hepatocytes SYTO® 16 green fluorescent nucleic acid DMSO 488 51 8 stain In vivo nuclear labeling of epithelia and endothelia, putative in vivo probe for apoptosis 15/ 30...

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Báo cáo sinh học: "A dual function fusion protein of Herpes simplex virus type 1 thymidine kinase and firefly luciferase for noninvasive in vivo imaging of gene therapy in malignant glioma" ppsx

Ngày tải lên : 14/08/2014, 19:22

... Cherrington JM: In vivo antitumor activity of SU11248, a novel tyrosine kinase inhibitor targeting vascular endothelial growth factor and platelet-derived growth factor receptors: determination of ... 122961 ± 22 155 Serial measurements (during and after GCV therapy) of tumor volumes and integrated light intensity units within a region of interest (ROI) including all tumors were plotted against each ... that fusion of the 27 kDa protein EGFP to HSV-TK allowed for predominant nuclear transfer of the enzyme while resulting in only minor loss of cytotoxic activity [22], suggesting that cytosolic localization...

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