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sơ lược kĩ thuật tạo hoa hồng xanh bằng kĩ thuật gene RNAi, hoa hông xanh có giá trị kinh tế cao tuy nhiên công nghệ này đang gặp phải vấn đề là kĩ thuật này vẫn chưa tạo ra một bông hồng xanh thật sự
BLUE ROSE TRANSGENIC PLANT Agenda Style 01 Introduction 02 method of gene transfer 03 applying Our Team Place Your Picture Here Ph.D Tran Thi Dung Van Pham Nhu Ngoc lecture student 618H0045@gmail.com 0587192774 Introduction In 2004, researchers used genetic modification to create roses that contain the blue pigment delphinidin 01 The blue rose is a flower of the genus Rosa (family Rosaceae) with the prefix from blue to purple 02 What is RNAi is involved in controlling gene activation in the post- RNAi? transcription phase • RNAi is the process of silencing a gene, inactivating a target gene with a sequence similar to that of the gene inactivating agent (short strands of RNA about 21-27 nucleotides) Basic features of the RNAi technique Specificity, high efficiency, very sensitive Inactivates genes of individuals at different stages, inherited from generation to generation, tissue to tissue The dsRNA design is easy if the genome sequence is known Method of gene transfer remove the gene encoding the enzyme dihydroflavonol reductase (DFR) in roses Application of RNAi technology for S ES KN EA ST RE N G TH S W gene replacement in plants created the 'silencer' gene to exploit a TH R E AT S complete the delphinidin-synthesis reaction TU R O PP O An iris gene for an enzyme, DFR, required to ES TI I N SWOT cellular phenomenon called RNA interference (RNAi) Creating blue roses using RNAi technique CHU TRÌNH HÌNH THÀNH SẮC TỐ TRÊN CÂY TRỒNG Anthocyanin dihydrokaempferol enzyme controls all three cycle of plant pigment formation including: cyanidin, pelargonidin and delphinidin RNAi technique to inhibit the activity of the DFR gene in red roses leads to inhibition of the delphinidin-transgene complete cyanidin the cycle with delphinidin a complete synthesis DFR gene cycle => using RNAi technique to inhibit the DFR gene clone a new delphinidin gene from pansy and DFR gene from iris flower DFR gene inhibition of roses without affecting the DFR gene of iris flowers Cell pH is one of the main reasons flowers have Petal ph cell concentrations the same anthocyanin cycle but have different colors Influence of are often genetic Ordinary rose petals have a pH While the cell pH is alkaline, the pigment of anthocyanin usually becomes greener concentration of about 4.5 Scientists have just come up with RNAi gene-suppressing pH Soil pH has little or no effect on the ph of the petal cells techniques to identify genes that affect petal acidity or adjust petal color in other directions 3 Applying The application of RNAi technology not only in creating blue roses but also opens up a new era for the application of Unique flower means making huge genetic engineering in agriculture 01 04 profits In addition to creating a blue rose, the scientist also creates a variety of flowers with different colors, In the global commission business with sales of 1.2 billion USD / year 02 05 The market for green roses is mainly in: America, Australia and Japan 03 fragrances and perils The demand for roses, cuttings accounts for 06 50% of the flower market Thank You Insert the Sub Title of Your Presentation ... technique to inhibit the DFR gene clone a new delphinidin gene from pansy and DFR gene from iris flower DFR gene inhibition of roses without affecting the DFR gene of iris flowers Cell pH... Inactivates genes of individuals at different stages, inherited from generation to generation, tissue to tissue The dsRNA design is easy if the genome sequence is known Method of gene transfer... in controlling gene activation in the post- RNAi? transcription phase • RNAi is the process of silencing a gene, inactivating a target gene with a sequence similar to that of the gene inactivating