VIN HN LM KHOA HC V CễNG NGH VIT NAM HC VIN KHOA HC V CễNG NGH - NGUYN TH NGOAN NGHIấN CU, TNG HP, C TRNG VT LIU LAI Vễ C (Ag, Fe3O4) HU C (CHITOSAN) CU TRC NANO NH HNG NG DNG TRONG Y SINH LUN N TIN S HểA HC H NI 2016 VIN HN LM KHOA HC V CễNG NGH VIT NAM HC VIN KHOA HC V CễNG NGH .*** NGUYN TH NGOAN NGHIấN CU, TNG HP, C TRNG VT LIU LAI Vễ C (Ag, Fe3O4) HU C (CHITOSAN) CU TRC NANO NH HNG NG DNG TRONG Y SINH LUN N TIN S HểA HC Chuyờn ngnh: Vt liu cao phõn t v t hp Mó s: 62440125 Ngi hng dn khoa hc: PGS.TS Trn i Lõm PGS.TS Nguyn Tun Dung H Ni 2016 LI CAM OAN Tụi xin cam oan nhng ni dung lun ỏn ny tụi thc hin di s hng dn ca ngi hng dn khoa hc Cỏc s liu, kt qu trỡnh by lun ỏn l trung thc v cha c cụng b lun ỏn khỏc Tỏc gi lun ỏn NCS Nguyn Th Ngoan LI CM N Trc ht tụi xin trõn trng by t lũng bit n sõu sc n PGS.TS Trn i Lõm, PGS.TS Nguyn Tun Dung ó tn tỡnh hng dn sut quỏ trỡnh hon nghiờn cu cng nh hon thin lun ỏn Tụi xin gi li cm n sõu sc n PGS.TS Phm Gia in cựng th phũng Cụng ngh cỏc cht cú Hot tớnh sinh hc - Vin húa hc; Phũng Nghiờn cu ng dng v trin khai cụng ngh - Vin K thut nhit i; GS Jeung Sang Go phũng thớ nghim trng im NExT MEMS Trng i hc Pusan Hn Quc ó to iu kin giỳp tụi hon thnh cỏc nhim v nghiờn cu cng nh hon thin bn lun ỏn Tụi xin gi li cm n chõn thnh n phũng sau i hc Vin K thut Nhit i, Khoa Húa hc - Hc vin Khoa hc v Cụng ngh - Vin Hn lõm Khoa hc v Cụng ngh Vit Nam ó to iu kin tụi hon thin lun ỏn Xin gi li cm n n gia ỡnh, bn bố ó ng viờn sut quỏ trỡnh thc hin lun ỏn! Tỏc gi lun ỏn Nguyn Th Ngoan MC LC DANH MC CC CH VIT TT DANH MC CC BNG DANH MC CC HèNH M U 11 CHNG TNG QUAN 13 1.1 Chitosan 13 1.1.1 Gii thiu chung v chitosan 13 1.1.2 Tng hp v tớnh cht ca chitosan 14 1.1.3 ng dng ca chitosan lnh vc y sinh 16 1.1.4 Curcumin 17 1.1.5 Nanocompozit chitosan/nano kim loi 18 1.2 Nanocompozit cha ht nano bc trờn nn chitosan 21 1.2.1 Ht nano bc 21 1.2.1 Cỏc tớnh cht c trng v ng dng 21 1.2.1.2 Cỏc phng phỏp ch to 24 1.2.2 Nanocompozit cha ht nano bc trờn nn chitosan (CS/AgNPs) 25 1.3 Nanocompozit cha ht nano st t trờn nn CS (CS/MNPs) 26 1.3.1 Ht nano st t 27 1.3.1.1 Cỏc tớnh cht v ng dng 28 1.3.1.2 Cỏc phng phỏp ch to 30 1.3.2 Nanocompozit cha ht nano st t trờn nn chitosan (CS/MNPs) 31 1.4 Nanocompozit cha ht nano bc v ht nano st t trờn nn chitosan (CS/AgNPs-MNPs) 34 1.4.1 Nanocompozit bc - st t (AgNPs-MNPs) 34 1.4.1.1 Cỏc tớnh cht v ng dng 36 1.4.1.2 Cỏc phng phỏp tng hp 38 1.4.2 Nanocompozit cha ht nano bc, ht nano st t trờn nn chitosan (CS/AgNPs-MNPs) 38 CHNG CC PHNG PHP NGHIấN CU V THC NGHIM 40 2.1 Nguyờn liu v húa cht 40 2.2 Phng phỏp thc nghim 40 2.2.1 Phng phỏp ch to nanocompozit cha nano bc trờn nn chiosan (CS/AgNPs) 40 2.2.1.1 Phng phỏp s dng cht kh NaBH4 41 2.2.1.2 Phng phỏp khụng s dng cht kh 41 2.2.1.3 Phng phỏp a nanocompozit CS/AgNPs lờn trờn b mt vi 42 2.2.1.4 Phng phỏp mang curcumin lờn vt liu CS/AgNPs 42 2.2.2 Ch to nanocompozit cha ht nano st t trờn nn chitosan (CS/MNPs) 43 2.2.2.1 Phng phỏp ng kt ta 43 2.2.2.2 Phng phỏp oxi húa kt ta 45 2.2.2.3 Phng phỏp phõn tỏn CS/MNPs CS bin tớnh vi cht phỏt quang 46 2.2.3 Ch to nanocompozit cha ht nano bc- st t trờn nn chitosan (CS/AgNPs-MNPs) 47 2.2.3.1 Phng phỏp ch to nanocompozit CS/AgNPs-MNPs 47 2.2.3.2 Phng phỏp mang curcumin lờn vt liu CS/AgNPs-MNPs 47 2.2.3.3 Thc nghim t nhit cm ng t 48 2.3 Cỏc phng phỏp nghiờn cu 49 2.3.1 Phng phỏp xỏc nh cu trỳc nanocompozit 49 2.3.1.1 Phng phỏp nhiu x tia X (XRD) 49 2.3.1.2 Phng phỏp ph tỏn sc nng lng tia X (EDX) 50 2.3.1.3 Phng phỏp ph hng ngoi (IR) 50 2.3.2 Phng phỏp xỏc nh hỡnh thỏi hc nanocompozit 50 2.3.2.1 Phng phỏp kớnh hin vi in t truyn qua (TEM) 50 2.3.2.2 Phng phỏp hin vi in t quột x trng FESEM 50 2.3.3 Phng phỏp nghiờn cu tớnh cht húa, lý, sinh ca nanocompozit 51 2.3.3.1 Phng phỏp t k mu rung (VSM) 51 2.3.3.2 Phng phỏp ph t ngoi kh kin (UV-vis) 51 2.3.3.3 Phng phỏp ph hunh quang 51 2.3.3.4 Phng phỏp th hot tớnh sinh hc 51 CHNG KT QU V THO LUN 53 3.1 Nanocompozit cha ht nano bc trờn nn chitosan (CS/AgNPs) 53 3.1.1 Ch to nanocompozit CS/AgNPs 53 3.1.1.1 Phng phỏp s dng cht kh NaBH4 53 3.1.1.2 Phng phỏp gia nhit 54 3.1.1.3 Phng phỏp s dng lũ vi súng 55 3.1.2 c trng cu trỳc 57 3.1.3 Hỡnh thỏi hc 60 3.1.4 Tớnh cht lý, húa, sinh hc 64 3.1.4.1 Tớnh cht quang 64 3.1.4.2 Hot tớnh sinh hc 65 3.1.5 Kh nng mang curcumin 68 Túm tt kt qu phn 3.1 70 3.2 Nanocompozit cha ht nano st t trờn nn chitosan 72 3.2.1 c trng cu trỳc 72 3.2.2 Hỡnh thỏi hc 74 3.2.3 Tớnh cht lý, húa, sinh hc 77 3.2.3.1 Tớnh cht t 77 3.2.3.2 Tớnh cht hunh quang 79 3.2.4 Kh nng ng dng y sinh ca CS/MNPs kờnh vi lu 81 Túm tt kt qu phn 3.2: 82 3.3 Vt liu nanocompozit cha nano st t, nano bc trờn nn chitosan (CS/AgNPs-MNPs) 83 3.3.1 Cu trỳc 84 3.3.2 Hỡnh thỏi hc 86 3.3.3 Tớnh cht húa, lý, sinh hc 87 3.3.3.1 Tớnh cht siờu thun t 87 3.3.3.2 Hot tớnh sinh hc 92 3.3.4 Kh nng mang curcumin 94 Túm tt kt qu phn 3.3 94 Kt lun chung 96 nh hng nghiờn cu tip theo 97 Mt s im mi ca lun ỏn 98 Danh mc cỏc cụng trỡnh cụng b ca nghiờn cu sinh 99 TI LIU THAM KHO 100 DANH MC CC CH VIT TT AgNPs Nano bc Bs Bacillus subtilis Ca Candida albicans CS Chitosan CS/NPs Nanocompozit cha nano kim loi trờn nn chitosan CS/AgNPs1 Nanocompozit cha nano bc trờn nn chitosan ch to bng phng phỏp kh NaBH4 CS/AgNPs2 Nanocompozit cha nano bc trờn nn chitosan ch to bng phng phỏp dựng chitosan iu kin gia nhit CS/AgNPs3 Nanocompozit cha nano bc trờn nn chitosan ch to bng phng phỏp dựng chitosan lũ vi súng CS/MNPs Nanocompozit cha nano st t trờn nn chitosan CS/MNPs1 Nanocompozit cha nano st t trờn nn chitosan ch to bng phng phỏp ng kt ta di dũng nit tr CS/MNPs2 Nanocompozit cha nano st t trờn nn chitosan ch to bng phng phỏp ng kt ta h vi lu CS/MNP3 Nanocompozit cha nano st t trờn nn chitosan ch to bng phng phỏp oxy húa kt ta CS-FITC Chitosan bin tớnh vi florescien isothiocyanat Cur Curcumin DCM Diclometan DD axetyl húa DOX Doxorubicin Ec Escherichia coli EDX Phng phỏp ph tỏn x nng lng FESEM Hin vi in t quột x trng FMNPs Nanocompozit nano st t trờn nn chitosan gn cht phỏt quang FITC Fluorescein isothiocyanat IC50 Nng c ch ti thiu 50% vi khun, vi nm La Lactobacillus fermentum MNPs Nano st t Fe3O4 MS T bóo hũa Pa Pseudomonas aeruginosa Sa Staphylococcus aureus Se Salmonella enteric SEM Hin vi in t quột TEM Hin vi in t truyn qua TLC Sc ký lp mng XRD Nhiu x tia X UV-vis Hp th ỏnh sỏng t ngoi vựng nhỡn thy VSM T k mu rung TI LIU THAM KHO Ti liu ting Vit Ngụ ỡnh Tun, (2007), Phỏt trin v s dng hp lý ngun ti nguyờn nc, Hi tho chuyờn v a dng sinh hc v bin i khớ hu: mi 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