PHỤ LỤC Phụ lục 1 Bảng phụ lục 1: Hoạt độ enzyme của 9 chủng vi khuẩn nuôi sục khí Chủng vi khuẩn Lần 1 Lần 2 Hoạt độ amylase (W 0 /ml) Hoạt độ protease (Hdp/ ml) Hoạt độ amylase (W 0 / ml) Hoạt độ protease (Hdp/ ml) 24 giờ 48 giờ 24 giờ 48 giờ 24 giờ 48 giờ 24 giờ 48 giờ 1 32 64 16 32 48 32 16 32 2 64 64 24 32 64 48 24 32 4 32 64 16 32 48 32 24 32 5 32 64 16 32 32 32 24 32 6 32 48 32 32 32 32 16 48 7 32 48 48 64 32 48 64 32 8 32 32 32 32 32 48 32 32 9 32 48 64 64 64 48 64 48 10 32 48 48 64 32 48 32 48 Bảng phụ lục 2: Hoạt độ enzyme của 9 chủng vi khuẩn nuôi không sục khí Chủng vi khuẩn Lần 1 Lần 2 Hoạt độ amylase (W 0 /ml) Hoạt độ protease (Hdp/ ml) Hoạt độ amylase (W 0 / ml) Hoạt độ protease (Hdp/ ml) 24 giờ 48 giờ 24 giờ 48 giờ 24 giờ 48 giờ 24 giờ 48 giờ 1 16 32 12 8 12 24 4 12 2 16 32 12 8 12 24 4 12 4 16 32 12 8 12 24 6 12 5 12 24 4 4 12 24 4 8 6 16 24 4 4 16 24 4 4 7 12 24 4 4 16 24 4 12 8 12 24 4 4 16 24 4 12 9 16 24 4 4 16 24 4 12 10 16 24 4 4 16 32 4 12 Bảng phụ lục 3: Hoạt độ enzyme của 4 chủng vi khuẩn trong 4 loại môi trƣờng Môi trƣờng Hoạt độ enzyme amylase Hoạt độ enzyme protease Chủng vi khuẩn Chủng vi khuẩn 2 7 9 10 2 7 9 10 Rỉ đường + 2% tinh bột 28 28 24 24 32 32 32 24 Rỉ đường + 1% tinh bột 18 18 18 20 24 16 24 24 Rỉ đường + 1% tinh bột +0,5%pepton 20 20 20 20 32 32 32 32 TSB + 1% tinh bột 14 14 16 14 20 20 20 20 Bảng phụ lục 4: Hoạt độ enzyme của 4 chủng vi khuẩn trong thí nghiệm 3 pH Thời gian 6,0 7,0 Hoạt độ amylase Hoạt độ protease Hoạt độ amylase Hoạt độ protease Chủng vi khuẩn Chủng vi khuẩn 2 7 9 10 2 7 9 10 2 7 9 10 2 7 9 10 24 giờ (n = 8) 28 24 28 28 12 12 12 16 32 32 32 32 24 16 16 16 48 giờ (n = 8) 32 32 32 32 24 16 16 16 32 48 32 32 24 16 16 16  A. Điều kiện sục khí và thời gian nuôi cấy Analysis of Variance for PHI1.ketquaa - Type III Sums of Squares Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level MAIN EFFECTS A:PHI1.CON 1023.1111 8 127.8889 1.612 .1558 B:PHI1.dksuckhi 9522.0000 1 9522.0000 120.025 .0000 C:PHI1.thoigian 1682.0000 1 1682.0000 21.202 .0000 INTERACTIONS AB 792.00000 8 99.000000 1.248 .3007 AC 264.00000 8 33.000000 .416 .9038 BC 50.00000 1 50.000000 .630 .4409 ABC 296.00000 8 37.000000 .466 .8715 RESIDUAL 2856.0000 36 79.333333 TOTAL (CORRECTED) 16485.111 71 0 missing values have been excluded. All F-ratios are based on the residual mean square error. Analysis of Variance for PHI1.ketquap - Type III Sums of Squares Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level MAIN EFFECTS A:PHI1.CON 2570.000 8 321.250 7.292 .0000 B:PHI1.dksuckhi 15901.389 1 15901.389 360.939 .0000 C:PHI1.thoigian 420.500 1 420.500 9.545 .0039 INTERACTIONS AB 3209.1111 8 401.13889 9.105 .0000 AC 326.0000 8 40.75000 .925 .5078 BC 93.3889 1 93.38889 2.120 .1541 ABC 485.1111 8 60.63889 1.376 .2398 RESIDUAL 1586.0000 36 44.055556 TOTAL (CORRECTED) 24591.500 71 0 missing values have been excluded. All F-ratios are based on the residual mean square error. Multiple range analysis for PHI1.ketquaa by PHI1.dksuckhi Method: 95 Percent LSD Level Count LS Mean Homogeneous Groups 0 36 20.111111 X 1 36 43.111111 X contrast difference limits 0 - 1 -23.0000 4.25873 * * denotes a statistically significant difference. Multiple range analysis for PHI1.ketquaa by PHI1.thoigian Method: 95 Percent LSD Level Count LS Mean Homogeneous Groups 24 36 26.777778 X 48 36 36.444444 X contrast difference limits 24 - 48 -9.66667 4.25873 * * denotes a statistically significant difference. Multiple range analysis for PHI1.ketquap by PHI1.dksuckhi Method: 95 Percent LSD Level Count LS Mean Homogeneous Groups 0 36 6.722222 X 1 36 36.444444 X contrast difference limits 0 - 1 -29.7222 3.17361 * * denotes a statistically significant difference. Multiple range analysis for PHI1.ketquap by PHI1.thoigian Method: 95 Percent LSD Level Count LS Mean Homogeneous Groups 24 36 19.166667 X 48 36 24.000000 X contrast difference limits 24 - 48 -4.83333 3.17361 * * denotes a statistically significant difference.  B. Loại môi trƣờng nuôi cấy Analysis of Variance for PHI2.kqa - Type III Sums of Squares Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level MAIN EFFECTS A:PHI2.con 2.00000 3 .66667 .023 .9951 B:PHI2.moitruong 546.00000 3 182.00000 6.276 .0051 INTERACTIONS AB 42.000000 9 4.6666667 .161 .9956 RESIDUAL 464.00000 16 29.000000 TOTAL (CORRECTED) 1054.0000 31 0 missing values have been excluded. All F-ratios are based on the residual mean square error. Analysis of Variance for PHI2.kqp - Type III Sums of Squares Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level MAIN EFFECTS A:PHI2.con 32.00000 3 10.66667 .267 .8484 B:PHI2.moitruong 832.00000 3 277.33333 6.933 .0033 INTERACTIONS AB 160.00000 9 17.777778 .444 .8906 RESIDUAL 640.00000 16 40.000000 TOTAL (CORRECTED) 1664.0000 31 0 missing values have been excluded. All F-ratios are based on the residual mean square error. Multiple range analysis for PHI2.kqa by PHI2.moitruong Method: 95 Percent LSD Level Count LS Mean Homogeneous Groups 4 8 14.500000 X 2 8 18.500000 X 3 8 20.000000 X 1 8 26.000000 X contrast difference limits 1 - 2 7.50000 5.70944 * 1 - 3 6.00000 5.70944 * 1 - 4 11.5000 5.70944 * 2 - 3 -1.50000 5.70944 2 - 4 4.00000 5.70944 3 - 4 5.50000 5.70944 * denotes a statistically significant difference Multiple range analysis for PHI2.kqp by PHI2.moitruong Method: 95 Percent LSD Level Count LS Mean Homogeneous Groups 4 8 20.000000 X 2 8 22.000000 X 1 8 30.000000 X 3 8 32.000000 X contrast difference limits 1 - 2 8.00000 6.70539 * 1 - 3 -2.00000 6.70539 1 - 4 10.0000 6.70539 * 2 - 3 -10.0000 6.70539 * 2 - 4 2.00000 6.70539 3 - 4 12.0000 6.70539 * * denotes a statistically significant difference  C. Điều kiện pH và thời gian nuôi cấy Analysis of Variance for PHI3.kqa - Type III Sums of Squares Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level MAIN EFFECTS A:PHI3.con 54.00000 3 18.00000 .474 .7049 B:PHI3.ph 162.00000 1 162.00000 4.263 .0555 C:PHI3.thigian 162.00000 1 162.00000 4.263 .0555 INTERACTIONS AB 150.00000 3 50.000000 1.316 .3038 AC 150.00000 3 50.000000 1.316 .3038 BC 2.00000 1 2.000000 .053 .8239 ABC 54.00000 3 18.000000 .474 .7049 RESIDUAL 608.00000 16 38.000000 TOTAL (CORRECTED) 1342.0000 31 0 missing values have been excluded. All F-ratios are based on the residual mean square error. Analysis of Variance for PHI3.kqp - Type III Sums of Squares Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level MAIN EFFECTS A:PHI3.con 198.00000 3 66.000000 2.200 .1278 B:PHI3.ph 50.00000 1 50.000000 1.667 .2150 C:PHI3.thigian 50.00000 1 50.000000 1.667 .2150 INTERACTIONS AB 38.000000 3 12.666667 .422 .7396 AC 38.000000 3 12.666667 .422 .7396 BC 50.000000 1 50.000000 1.667 .2150 ABC 38.000000 3 12.666667 .422 .7396 RESIDUAL 480.00000 16 30.000000 TOTAL (CORRECTED) 942.00000 31 0 missing values have been excluded. All F-ratios are based on the residual mean square error. Multiple range analysis for PHI3.kqa by PHI3.ph Method: 95 Percent LSD Level Count LS Mean Homogeneous Groups 6 16 29.500000 X 7 16 34.000000 X contrast difference limits 6 - 7 -4.50000 4.62137 * denotes a statistically significant difference. Multiple range analysis for PHI3.kqa by PHI3.thigian Method: 95 Percent LSD Level Count LS Mean Homogeneous Groups 24 16 29.500000 X 48 16 34.000000 X contrast difference limits 24 - 48 -4.50000 4.62137 * denotes a statistically significant difference.  D. Thời gian và nhiệt độ bảo quản chế phẩm Analysis of Variance for PHI5.kqa - Type III Sums of Squares Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level MAIN EFFECTS A:PHI5.con 279.500 3 93.167 248.444 .0000 B:PHI5.nhietdobq 4.000 1 4.000 10.667 .0026 C:PHI5.thoigianbq 31417.000 3 10472.333 27926.222 .0000 INTERACTIONS AB 3.50000 3 1.166667 3.111 .0400 AC 772.50000 9 85.833333 228.889 .0000 BC 2.00000 3 .666667 1.778 .1712 ABC 4.50000 9 .500000 1.333 .2592 RESIDUAL 12.000000 32 .3750000 TOTAL (CORRECTED) 32495.000 63 1 missing values have been excluded. All F-ratios are based on the residual mean square error. Analysis of Variance for PHI5.kqp - Type III Sums of Squares Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level MAIN EFFECTS A:PHI5.con 432.000 3 144.0000 2.250 .1015 B:PHI5.nhietdobq .000 1 .0000 .000 1.0000 C:PHI5.thoigianbq 17328.000 3 5776.0000 90.250 .0000 INTERACTIONS AB .0000 3 .00000 .000 1.0000 AC 1296.0000 9 144.00000 2.250 .0443 BC .0000 3 .00000 .000 1.0000 ABC .0000 9 .00000 .000 1.0000 RESIDUAL 2048.0000 32 64.000000 TOTAL (CORRECTED) 21104.000 63 1 missing values have been excluded. All F-ratios are based on the residual mean square error. Multiple range analysis for PHI5.kqa by PHI5.thoigianbq Method: 95 Percent LSD Level Count LS Mean Homogeneous Groups 20 16 4.750000 X 30 16 4.750000 X 10 16 5.000000 X 0 16 56.000000 X contrast difference limits 0 - 10 51.0000 0.44111 * 0 - 20 51.2500 0.44111 * 0 - 30 51.2500 0.44111 * 10 - 20 0.25000 0.44111 10 - 30 0.25000 0.44111 20 - 30 0.00000 0.44111 * denotes a statistically significant difference. Multiple range analysis for PHI5.kqa by PHI5.nhietdobq Method: 95 Percent LSD Level Count LS Mean Homogeneous Groups 30 32 17.375000 X 4 32 17.875000 X contrast difference limits 4 - 30 0.50000 0.31191 * * denotes a statistically significant difference. Phụ lục 2:  Môi trƣờng Trypticase Soya Agar (TSA) Thành phần g/l Soy pepton 15 g Tryptone peptone 5 g NaCl 5 g Agar 18 g Nước cất 1000 ml pH 7,3 ± 0,2 Cân 30 g bột môi trường TSB + 18 g agar cho vào 1000 ml nước cất, đun sôi cho hòa tan. Đem hấp khử trùng ở nhiệt độ 121 0 C/ 15 phút.  Môi trƣờng Tryticase Soya Broth (TSB) Thành phần g/l Soy pepton 15 g Tryptone peptone 5 g NaCl 5 g Nước cất 1000 ml pH 7,3 ± 0,2 Cân 30 g bột môi trường TSB cho vào 1000 ml nước cất, đun sôi cho hòa tan. Đem hấp khử trùng ở 121 0 C/15 phút. . MAIN EFFECTS A:PHI2.con 2.00000 3 .66 667 .023 .9951 B:PHI2.moitruong 5 46. 00000 3 182.00000 6. 2 76 .0051 INTERACTIONS AB 42.000000 9 4 .66 666 67 . 161 .99 56 RESIDUAL 464 .00000 16 29.000000 . A:PHI3.con 198.00000 3 66 .000000 2.200 .1278 B:PHI3.ph 50.00000 1 50.000000 1 .66 7 .2150 C:PHI3.thigian 50.00000 1 50.000000 1 .66 7 .2150 INTERACTIONS AB 38.000000 3 12 .66 666 7 .422 .73 96. 12 .66 666 7 .422 .73 96 AC 38.000000 3 12 .66 666 7 .422 .73 96 BC 50.000000 1 50.000000 1 .66 7 .2150 ABC 38.000000 3 12 .66 666 7 .422 .73 96 RESIDUAL 480.00000 16 30.000000 TOTAL (CORRECTED) 942.00000