CHAPTER ENZYME CONTENTS  Nomenclature and Classification  Mechanism of enzyme action  Catalytic mechanism  Enzyme activity  Factors influence on enzyme activity  Enzyme Kinetics  Enzyme production 04/06/2023 ENZYME NOMENCLATURE  Traditional name: some of the earliest enzymes to be discovered:  Name: ending in – “in” to indicate they were proteins  Ex: the digestive enzymes: pepsin, trypsin, chymotrypsin 04/06/2023 ENZYME NOMENCLATURE  Common name Substrate/ activity + ase  Examples:  urease catalyzes hydrolysis of urea  DNA polymerase catalyzes the polymerization of nucleotides to form DNA 04/06/2023 EXAMPLES 04/06/2023 ENZYME NOMENCLATURE  EC (Enzyme Commission) systematic name:  [International Union of Biochemistry and Molecular Biology: IUBMB]  Systematic name indicating:  The substance acted on  The functional group acted on  The type of reaction catalyzed  All EC names end in –ase 04/06/2023 ENZYME NOMENCLATURE Systematic name: encoded by a four-digit classification number  Ex: EC number: 2.7.1.1.:  (2) : the class name (transferase)  (7): the sub class (phosphotransferase)  (1): the sub sub class (a phosphotransferase with a hydroxyl group as acceptor)  (1): the sub sub sub class (D-hexose as the phosphoryl group acceptor) 04/06/2023 EC CLASSIFICATION OF ENZYMES Enzymes: grouped into major classes (based on the rxns which they catalyze) 04/06/2023 ENZYME CLASSIFICATION 04/06/2023 ENZYME CLASSIFICATION 04/06/2023 10