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PowerPoint Presentation Enzyme specificity Absolute Linkage digestive enzyme Group carboxypeptidase Stereo malatdehydratase 2 Absolute specificity Urease Arginase Renin Application 3 Lipase Protease A[.]
Enzyme specificity • Absolute • Linkage: digestive enzyme • Group: carboxypeptidase • Stereo: malatdehydratase Absolute specificity • Urease • Arginase • Renin Application Linkage • Lipase • Protease • Amilase (α- , β- , -) Application Group • Aminpeptidase • Carboxypeptidase • NADH dehydrogenase stereospecific subclass Hydrolase – 3.1 Esterase – – – – • 3.1.1 Ester carbocylic 3.2 3.3 3.4 3.5 Glucosidase Etherase Peptidase Amidase Enzym code: digits Lipase (EC 3.1.1.3) Trypsin (EC 3.4.21.4) Alcoholdehydrogenase (EC 1.1.1.1) Aldolase (EC 4.1.2.13) Catalase (EC 1.11.1.6) Starch hydrolyzing enzyme • α- amilase: most thermal stable, • β- amilase: acid stable Temp max: 700C • - amilase: working pH: 3,5-5,5 peptide hydrolase • Endo-peptidase (proteinase): pepsin, trypsin, chimotrypsin – Serin Protease – Thiol Protease – Acid Protease (aspartic proteinase) – Metalo Protease • Exo-peptidase (peptidase) – Amino peptidase – Cacboxyl peptidase Application of hydrolysing enzyme • amilase (-,-,-amilase) • protease (animal, plant, microorganism) • Lipase • Pectinase • Cellulase • Others Oxydoreductase • • • • Dehydrogenase Polyphenoloxidase Catalase Peroxidase