[...]... terms of p change andlor turbidity and they canbe present inlow Cell and Tissue Culture: ~ a b o r ~ tProcedures in ~ i o t ~ c ~ n o ledited , by A.Doyle and J.B Griffiths ~ry o~y 0 1998 John Wiley & Sons Ltd 4 THE CELL: SELECTION AND STANDARDI~ATION numbers ~ycoplasmas elicit numerous deleterious effects and their presence is incompatible with standard~edsystems Routinely,brothand agar cultureor echst... & 4.7 Pharmacia & Upjohn AB, Strandbergsgatan 47, S-112-87 Stockholm, Sweden Section: 3.4 XiV Barbara Clough National Institute for Medical Section:1.2 CELL AND TISSUE CULTURE Research, Mill Hill, London NW7 3.AA, U Martin Clynes National Cell and Tissue Culture Centre, Bioscience Ireland, School of Biological Sciences, Dublin City University, Clasnevin, Dublin 9, Ireland Section: 2.6 Thomas C Cotter... t r i a l ~ ni Estate, Section: 4.4 atoire des Sciences G r a ~ ~ ~ i 54001 lle, Cedex, France Section: 4.3 xvi CULTURE CELL AND TISSUE Angela Martin National Cell and Tissue Culture Centre, Bioscience Ireland, School of Biological Sciences, Dublin City University, Glasnevin, Dublin 9, Ireland Section: 2.6 Jennie P Mather Genentech Inc., 460 San Bruno Boulevard, San Francisco, CA 94080, USA Section:... University College Cork, Lee Maltings, Prospect Row, Cork, Ireland Section: 4.4 Irene Cour American Type Culture Collection, Cell Culture ~epartment, PO Box 1549, Manassas, Virginia 20108 3 549, USA Section:1.8 National Cell and Tissue Culture Centre, Bioscience Ireland, School of Biological Sciences, Dublin City University, Glasnevin, Dublin Ireland Section: 2.6 9, ogram~e Manager, Joint I~frastructure... receptor and insulin-like growth factor The cells are used extensively in pharmacoto~icology studies Cells are cultured in DMEM with 10% FBS The cells are highly contact inhibited and are sensitive to serum batches This is a widely studied cell line derived from a human cervix adenocarcinoma (Gey et al., 1952) The cells are epithelial-like in morphology and are susceptible to polio virus type 1and adenovirus... partners for Ig-secreting spleen cells to obtain hybrids secreting only the specific antibody (hybridoma).The myeloma cells of Sp210-Ag14 appear to fusepreferentially with the dividing cells of the spleen, which, of the l3 cells, are principally the Ig-secreting, pla~ue-formingcells Cells are cultured in R I 1640 with glutamine and 10% Fl3S The cells are resis8-azaguanine and die in the presence of HAT... polio, rabies and yellow fever The cells are anchorage dependent but will form a multilayered culturewhen held for long periods at 3'7°C with periodic p H a d j u s t ~ ~ nThe cells have a life span of ~ 0 ~ 1 0 ts popul~tion doublings, with a doubling time of 24h S Cells are cultured in Eagle's basal medium with 10% F 12 THE CELL: SELECTION ANDSTANDARDI~ATION Human B-lymphoblastoid cell line Namalwa... adenovirus and adenovirus ovirus vectors are being usedincreasingly for many ation andgene therapy; 293cells canbe used to isolate transformation-defective host-range mutants of Ad5 and for tit rat in^ human adenovir~ses The 29 3cell Cells are cultured in Eagle’s supplemented with 10% horse serum or The cells detach at room temperature and may take days to reattach ics line Principal characteristics and applications... The topic is a to say that once minimum standards are set they can be all-embracing for every cell type handled f articular importance in the routine handling of cell cultures is the plasma contamination status cell lines.If present, the concentration of mycoplasof mas in the culture supernatant can be in the region of 106-108 mycoplasmas ml-l Unlike bacterial and fungal contaminants, they do not necessarily... (Nelson-Rees et al., 1981) CELL LINES FOR ~IOTECHNOLO~ISTS 9 Cells are cultured in Eagle’s MEM (EBSS) supplemented with glutamine, nonessential amino acids and 10% FBS This is a mouse BALBlc myeloma cell line secreting IgA (Halpern &k Coffman, 1972; Lundblad et al., 1972; Oi et al., 1983) The cell line is used in transfection studies eo S Cells are cultured in DME The Verocell line was derived from . 7 Cell line NIH3T3 Cell line HeLa Cell line J558L Cell line Vero Myeloma cell lines Hybridomas Cell line MRC-5 Cell line WI-38 Cell line Namalwa Cell line BHK-21 Cell line MDCK Cell. xvi CELL AND TISSUE CULTURE Angela Martin National Cell and Tissue Culture Centre, Bioscience Ireland, School of Biological Sciences, Dublin City University, Glasnevin, Dublin 9, Ireland. Cell line GH3 Cell line 293 Cell line !VCRE/!VCRIP References Master and Working Cell Banks Scale and composition of cell banks Extended cell bank The cell banking environment and procedures