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Antioxidant Activity of the Flavonoid Hesperidin in Chemical and Biological Systems Antioxidant Activity of the Flavonoid Hesperidin in Chemical and Biological Systems Scientific seminar Lam Thu Huyen K63D2 Phamarceutical Chemistry Ha Noi university of Science I Background I Background General about hesperidin Wilmsen P ; J Agric Food Chem 2005, 53, 4757−4761 Hesperidin I Background I Background properties of hesperidin Wilmsen P ; J Agric Food Chem 2005, 53, 4757−4761 antiallergenic anticarcino.
Antioxidant Activity of the Flavonoid Hesperidin in Chemical and Biological Systems Scientific seminar - Lam Thu Huyen K63D2 - Phamarceutical Chemistry - Ha Noi university of Science I.Background: General about hesperidin Hesperidin Wilmsen P.; J Agric Food Chem 2005, 53, 4757−4761 I.Background: properties of hesperidin antiallergenic anticarcinogenic vasodilator properties antihypotensive antimicrobial Wilmsen P.; J Agric Food Chem 2005, 53, 4757−4761 I.Background: hesperidin's ability to scavenge free radicals and reactive oxygen species General about free radicals cancer cell Wilmsen P.; J Agric Food Chem 2005, 53, 4757−4761 I.Background: hesperidin's ability to scavenge free radicals and reactive oxygen species Experiment and result: orange juice mandarin juice → reduce in colon and lung cancer → study the antioxidant activity of hesperidin Wilmsen P.; J Agric Food Chem 2005, 53, 4757−4761 I.Background: method used to evaluate antioxidant capacity of hesperidin Method: in vitro Wilmsen P.; J Agric Food Chem 2005, 53, 4757−4761 II.Problem In vivo Wilmsen P.; J Agric Food Chem 2005, 53, 4757−4761 III.Methods & results: modified Yamaguchi et al method • using a modified Yamaguchi et al method → compare hesperidin with trolox - an antioxidant 250 µM of DPPH (radical) hesperidin & trolox dissolved in ethanol + Tris-HCl (diluted in DMSO) buffer ( difference concentration) Wilmsen P.; J Agric Food Chem 2005, 53, 4757−4761 maintained in dark 20min befor using spectrophometry method at 517 nm III.Methods & results: modified Yamaguchi et al method • result: reduced the level of the DPPH٠ in a similar way to the antioxidant trolox ( positive control) except 10 µM higher capacity of reducing the DPPH radical than trolox Figure DPPH٠ concentration reduced by hesperidin (■) and trolox () Wilmsen P.; J Agric Food Chem 2005, 53, 4757−4761 III Methods & results: using yeast cells Target Yeast use in method Evaluation of antioxidant capacity using yeast cells Advantages of method testing of various products fast sensitive reproducible reliable results Saccharomyces cereVisiae • possesses a variety of antioxidant defenses • in experiment: using superoxide-dismutase ( SOD) proficient and deficient strains Wilmsen P.; J Agric Food Chem 2005, 53, 4757−4761 III Methods & results: using yeast cells Procedure × 106 cells/mL exponential-phase hesperidin in DMSO S.cereVisiae ( 25 and 50 µM) cells Wilmsen P.; J Agric Food Chem 2005, 53, 4757−4761 stressing agents 1mM (paraquat herbicide & hydrogen peroxide) III Methods & results: using yeast cells incubated in YPD medium 6h, 28°C, 130 rpm diluted in 0.9% (w/v) NaCl, plated onto YPD agar incubated 72h, 28°C compare with control plates (beginning) Wilmsen P.; J Agric Food Chem 2005, 53, 4757−4761 III Methods & results: using yeast cells Result • protect cells from damage induced by stressing agents Table Survival of SOD Proficient and Deficient S cerevisiae Strains Treated and Untreated with the Antioxidant Hesperidin and/or the Stressing Agents Paraquat or Hydrogen Peroxide Wilmsen P.; J Agric Food Chem 2005, 53, 4757−4761 IV Conclusions & discussions • • the significant antioxidant activity of hesperidin both in vitro and in vivo assay hesperidin can be considered as a potentially active compound for use in conditions where reactive oxygen species are implicated Wilmsen P.; J Agric Food Chem 2005, 53, 4757−4761 ... scavenge free radicals and reactive oxygen species Experiment and result: orange juice mandarin juice → reduce in colon and lung cancer → study the antioxidant activity of hesperidin Wilmsen P.;... maintained in dark 20min befor using spectrophometry method at 517 nm III.Methods & results: modified Yamaguchi et al method • result: reduced the level of the DPPH٠ in a similar way to the antioxidant. .. using yeast cells Result • protect cells from damage induced by stressing agents Table Survival of SOD Proficient and Deficient S cerevisiae Strains Treated and Untreated with the Antioxidant Hesperidin