... Publisher. All rights reserved Research Paper Prevalence of Overactive Bladder, its Under-Diagnosis, and Risk Factors in a Male Urologic Veterans Population Wellman W Cheung1, William Blank1, Dorota ... population is under-diagnosed and under-treated. Key words: overactive bladder, OAB, incontinence, male, urology, veterans INTRODUCTION Recent internat...
... once daily for 7 days, starting from 1 day before CCI surgery. Evaluation of tactile allodynia and thermal hyperalgesia The paw withdrawal latency (PWL) to radiant heat and paw withdrawal threshold ... Neuropathic pain is characterized by hyperalge-sia, allodynia and spontaneous pain. It often occurs as a result of injury to peripheral nerves, dorsal root ganglions (DRG), spinal c...
... a glycoprotein
(80 kDa) present in blood plasma with a concentration of
35 l
M
.Itisonly30%saturatedwithironinbloodplasma
and has the capacity for binding to other metal ions of
therapeutic and ... occurred. Although the signals were
broadened and shifted by Yb
3+
due to its paramagnetic
character, the appearance of a new signal at 2.120 p.p.m.
and the splitting of s...
... of domains
other than the Gla domain on membrane binding, we have
now investigated the membrane-binding properties of
coagulation factor X and Gla domain-containing frag-
ments of this protein.
MATERIALS ... binding of factor X and its Gla-containing fragments to membranes containing 25 mol% PtdSer in the presence of
10 m
M
Ca
2+
obtained by evaluation of association and...
... Evaluation of two biosynthetic pathways to d-aminolevulinic acid
in
Euglena gracilis
Katsumi Iida, Ippei Mimura and Masahiro Kajiwara
Department of Medicinal Chemistry, Meiji Pharmaceutical ... University, Kiyose-shi, Tokyo, Japan
d-Aminolevulinic acid (ALA), which is an intermediate in
the b iosynthesis o f c hlorophyll a, c an be biosynthesized via
the C5 pathway and the Shemin...
... 5¢-AAAAATGTTGCTGCC
CTG-3¢ for syndecan-4; 5¢-GAATGACTCGGAGCGTAC
ACTG-3¢ and 5¢-CCTTTGAGCACATTTCGGCAA-3¢
for glypican-1; 5¢-ACAGACTACCTCATGAAGAT-3¢
and 5¢-AGCCATGCCAAATGTCTCAT-3¢ for b-actin.
The ... regulation is
mainly transcriptional [8].
Syndecans and glypicans bind a variety of extracellular
matrix molecules and growth factors in a heparan sulfate
dependent manner [15]....
... [36], and pyruvate kinase [6].
Inhibition of pyruvate kinase. This enzyme catalyses the
regeneration of ATP from ADP and PEP, in the last step of
glycolysis. Due to its physiological relevance, pyruvate
kinase ... PEP analogues 1c,d, 2g and 3c in the
presence of Mg
2+
, and were then assayed for residual
catalytic activity with their natural substrates. Inactivation
of...
... frozen at )80 °C until the
insulin assays. Insulin was measured by the radioimmun o-
assay method of Herbert et al. [15], using rat insulin as a
standa rd.
RNA isolation and Northern-blot analysis
Total ... Ontogenesis of hexokinase I and glucokinase protein in rat pancreatic islets and liver. Western-blot analyses of glucokinase in pancreatic islets
(A) and liver (B) an...
... use.
Determination of branching enzyme activity on amylose
and amylopectin with the starch–iodine assay
Amylose (type III, Sigma) and amylopectin (Sigma) from
potato were typically dissolved at 10 mg
:
mL
21
in ... added to obtain increasing concen-
trations of phosphate. Incubations were terminated at
120 min and analysed by the starch –iodine assay.
Incubation of linear dextri...
... loop for DNA
binding by RecA ⁄ Rad51 family proteins was observed
by mutational and photocrosslinking analyses of RecA
[34–37] and a mutational analysis of HsDmc1 [22].
The Dmc1-F23 3A mutation, which ... compilation ª 2006 FEBS 3159
Roles of the human Rad51 L1 and L2 loops in DNA binding
Yusuke Matsuo
1
, Isao Sakane
2
, Yoshimasa Takizawa
1
, Masayuki Takahashi
3
and Hito...