Báo cáo y học: "Expression of Human Globular Adiponectin-Glucagon-Like Peptide-1 Analog Fusion Protein and Its Assay of Glucose-Lowering Effect In Vivo"
... protein. Most of the protein was expressed in inclusion body. The fusion protein in inclusion body was purified by using High-Affinity Nickel Iminodiacetic Acid Resin and refolded in urea gradient ... of Human Globular Adiponectin-Glucagon-Like Peptide-1 Analog Fusion Protein and Its Assay of Glucose-Lowering Effect In Vivo Tongfeng Zhao1, Jing...
... Serotonin (5-hydroxytryptamine) interacts
with N-acetylneuraminic acid, which is often contained
in glycolipids and glycoproteins on the cell surface
[24]. We examined the synergistic effects of ... (1986) Involvement of
finger domain and kringle 2 domain of tissue-type plas-
minogen activator in fibrin binding and stimulation of
activity by fibrin. EMBO J 5, 3525–3530.
37 Bakk...
... layers of reticular subtype of oral lichen planus patient (streptavidin-biotin amplified system, x 400). Figure 2. Increased labelling of hMSH2 protein in basal and intermediate epithelial layers ... counterstained with Meyer’s haematoxylin and mounted in PermountTM. Negative controls consisted of omission of the primary or the secondary antibody or primary incubation in...
... classes.
Role of Tyr183 in catalysis
In previous crystallographic studies it was shown that the
hydroxyl side chain of Tyr183 is within hydrogen bonding
distance of the N-acetyl carbonyl oxygen of the ... activity of the pure recombinant protein was
80% of that of t he wild-type protein in both the lysozyme
and chitinase assays. Attempts to further purify the
recombi...
... the combination of the isoforms is yet to be determined.
We are now trying to express fusion proteins of
A. acetabulum proteolipid subunits and green fluorescent
protein in A. acetabulum and tobacco ... complementability of the group 2
proteins for the yeast vma3-mutant. A comparison of the
structures, especially in the four hydrophobic domains
(underlined sequences in Fi...
... PRISM
(http://www.graphpad.com).
Single turnover assay
Single turnover assays of the individual components
(10nmolofTomoH,20nmolofTomoCandTomoD
subunits) and of each of their possible combinations, were
performed by adding ... mass of about 10, 38 and 57 kDa, the
expected molecular size of recombinant subunits B, E and
A, respectively. The identity of the proteins was furt...
... acid synthesis. Indeed, synthesis of blood coagulation
proteins in vertebrates and toxins in the cone snail may be
secondary functions of this enzyme.
ACKNOWLEDGEMENTS
We especially appreciate ... (1997) The
active site region of the vitaminK-dependent carboxylase includes
both the amino-terminal hydrophobic and carboxy-terminal
hydrophilic domains of the protein. FEBS Lett....
... after 40 cycles
consisting of 1 min at 94 °C, 1 min at 44 °Cand1minat
72 °C in a Hybaid thermocycler. Subcloning in pZERO
(Invitrogen) using EcoRI and BamHI resulted in loss of a
part of the amplified ... guanine
nucleotide-binding proteins: the G proteins. G proteins
play a central role in a wide variety of signal transduction
pathways, mediating the perception of environm...
... the site of injection
Injection of microorganisms resulted in a dramatic decrease
in numbers of both circulating and tissue in ltrating
haemocytes within 3 h of injection. To study haemocyte
behaviour ... immunoreactivity observed at the site of
microbial injection precluded both clarification of the
mechanisms of penaeidin release from haemocytes, and
determination of...
... Kuhne,
Pierre Flandin, Jean-Paul Giacobino and Patrick Muzzin
Department of Medical Biochemistry, Faculty of Medicine, University of Geneva, Switzerland
Uncoupling protein- 3 (UCP3) is a mitochondrial inner-
membrane ... specific and total levels of
COX protein were not significantly different in IMF and SS
mitochondria. The yield in mitochondria, which was
determined by co...