... autoradio-
graphy.
In vitro
transcription, translation, targeting
and cross-linking analysis
To generate truncated mRNA, plasmids (Table 1) encoding
truncated nascent chains were linearized and transcribed ... translocon [5,6]. At the onset of
translocation, SecB is released [7] and the preprotein is
translocated by an insertion–deinsertion cycle of SecA into
the SecYEG translocon...
... formation of
a- hydroxyhaem with haem oxygenase may be small and a
substantial amount of free a- hydroxyhaem may remain,
depending on the reconstitution conditions; this could lead
to a misinterpretation ... the
precipitation of free a- hydroxyhaem. These observations
indicated that a- hydroxyhaem might be apt to aggregate
during incubation at neutral pH, and consequently,...
... Further identification and quantification
of puromycin were achieved by a Pac enzymatic assay
[21].
Preparation of 3¢-amino-3¢-deoxyadenosine
3¢-amino-3¢-deoxyadenosine was obtained from Helmin-
thosporium ... letters.
The start and direction of each ORF are
indicated by horizontal arrows and named
accordingly. Putative )10 and )35 regions of
ata12 and ataPKS1 are overlined....
... the pyridoxal isonicotinoyl hydrazone class as
effective antiproliferative agents II. The mechanism of action of
ligands derived from salicylaldehyde benzoyl hydrazone and
2-hydroxy-1-naphthylaldehyde ... a plastic spatula to detach them.
Radioactivity was measured in both the cell pellet and
supernatant using a c-scintillation counter (LKB Wallace
1282 Compugamma, Finland).
Dete...
... CTTGCATGCCCTGCAGGTCG
Mutagenic L73G Forward GTATTCAAAAGTGGTCCCGGACAAAATGAG GACTTG
Reverse TCCGGGACCACTTTTGAATACTTTCAAGTGCATATATTTATT
P74G Forward CAAAAGTCTTGGCGGACAAAATGAGGACTTGGTAC
Reverse CATTTTGTCCGCCAAGACTTTTGAATACTT ... function as the second binding loops of cystatin B and
family 2 cystatins and also what residues of this loop in
cystatin A may participate in the interacti...
... mitoch ondrial F
1
-ATPase and inhibits
catalysis is bound at a catalytic site. Biochim. Biophys. Acta 1020,
43±48.
11. Muneyuki, E., Makino, M., Kamata, H., K agawa, Y ., Y oshida,
M. & Hirata, ... Masasuke Yoshida
Chemical Resources Laboratory, Tokyo Institute of Technology, Japan
F
1
-ATPase i s inactivated by entrapment of MgADP in
catalytic sites and reactivated by Mg...
... concentration of the reagent.
Table 1. Inactivation of Gra3P DH of EAC cells and rabbit muscle by TNBS or PP and reactivation of the enzymes by thiol-containing
compounds. Approximately 5 U of the EAC ... modification of a unique lysine residue of EAC cell
Gra3P DH and of a cysteine residue of rabbit muscle
Gra3P DH.
Protection of the activity of EAC cell Gra3
P...