... detected in controlplacenta, whole human skin, normal epidermal and immor-talized keratinocytes, dermal fibroblasts, squamous cellcarcinoma and five human melanomas. Thus, these dataclarify in detail ... plate w ithchloroform/methanol (1 : 1, v/v ), and the associatedradioactivity measured by scintillation counting.Side-chain cleavage of 7-DHC by placental and adrenalmitochondria. Incubations ... theagarose gel and purified using a GFX PCR DNA and gelband purification kit (Amersham-Pharmacia-Biotech).PCR fragments were cloned i n pGEM-T easy vector system (Promega) and purified with a plasmid...