GENERATION AND CHARACTERIZATION OF HUMAN MONOCLONAL ANTIBODIES WITH NEUTRALIZING ACTIVITY FOR DENGUE VIRUS
GENERATION AND CHARACTERIZATION OF HUMAN MONOCLONAL ANTIBODIES WITH NEUTRALIZING ACTIVITY FOR DENGUE VIRUS
... classification for dengue severity The new classification for dengue severity is divided into Dengue without Warning Signs, Dengue with Warning Signs, and Severe Dengue 24 1.2 Molecular Biology of DENV ... Fisher and Prof Leo Yee Sin, thank you for recruiting patients for our study To Prof Mary Ng and Boon, thank you for providing us with technical advice and...
The generation of native human monoclonal antibodies with neutralising activity for dengue virus 2
... 1.1.5.4 Virus assembly and propagation 17 1.1.6 Phylogeny of Dengue Virus 19 1.1.7 Pathogenesis of Dengue Virus 22 1.1.8 Immune response to Dengue Virus 26 1.1.8.1 Innate immunity to DV 26 1.1.8 .2 ... culture 62 2.8 Source of primary CD 22+ B cells 63 2. 9 RNA extraction and RT-PCR for serotyping of patients 63 2. 10 Cloning of B cells from Dengue virus- i...
The generation of native human monoclonal antibodies with neutralising activity for dengue virus 3
... on the surface of the virus There are a number of different mechanisms postulated to prevent virus from entering the cell One way is for antibodies to alter the spatial distances 33 between the ... 6.0) of the trans Golgi network triggers dissociation of the prM/E heterodimers, which leads to the formation of 90 dimers that lie flat on the surface of th...
Báo cáo y học: " Generation and characterization of high affinity human monoclonal antibodies that neutralize staphylococcal enterotoxin B" ppsx
... al.: Generation and characterization of high affinity human monoclonal antibodies that neutralize staphylococcal enterotoxin B Journal of Immune Based Therapies and Vaccines 2010 8:9 Submit your ... HuMAbs neutralize SEB-induced cytokine production by human lymphocytes To examine the biological activity of HuMAbs in vitro, a cell-based assay was employed t...
Báo cáo khoa học: Engineering and characterization of human manganese superoxide dismutase mutants with high activity and low product inhibition potx
... hMnSOD has a low level of product inhibition, similar to that of the parent mutant Q143A hMnSOD, while exhibiting higher catalytic activity and efficiency Although even higher catalytic activity would ... efficiency and similarly low product inhibition compared with the Q143A hMnSOD parent Our results demonstrate the ability of directed evolution to engineer variants...
Báo cáo khoa học: Isolation and characterization of an IgNAR variable domain specific for the human mitochondrial translocase receptor Tom70 potx
... degree of affinity and specificity for Tom70, and with a KD of nM compares very well with affinities reported for camelid VHH domains (that vary in the range 2–300 nM [36]) and for scFv and disulphide ... generate The human Tom70 receptor is an important example of such refractory targets, and the generation of a high-affinity reagent specific for the...
Báo cáo y học: "The critical role of arginine residues in the binding of human monoclonal antibodies to cardiolipin" ppsx
... a single experiment only are shown in Figs 2,3,4 The importance of arginine residues in IS4VH As reported previously, the presence of the heavy chain of IS4 plays a dominant role in binding to ... 94 in CDR3 of UK4VL hinders DNA binding sterically A similar effect may be occurring with regards to the binding of UK4VL to CL The effect of point mu...
Báo cáo y học: " Full genome comparison and characterization of avian H10 viruses with different pathogenicity in Mink (Mustela vison) reveals genetic and functional differences in the non-structural gene" pot
... Zohari et al., Full genome comparison and characterization of avian H10 viruses with different pathogenicity in Mink (Mustela vison) reveals genetic and functional differences in the non-structural ... of the influenza virus [19] The amino acid composition of the receptor binding pocket of the HA protein for the H10 isolates...
Báo cáo y học: "Isolation and characterization of human cells resistant to retrovirus infection" pdf
... that one of the blocks to CYTOPLASM NUCLEUS Figure mutant 42-7 localization of proviral DNA in wild type and Synthesis andcells Synthesis and localization of proviral DNA in wild type and mutant ... efficiency of single round infection in cells of differing types and species [11,12] Therefore, to build upon and extend the rodent cell studies, and to identify cell...
Engineering and characterization of human renal proximal tubular cells for applications in vitro toxicology and bioartifical kidneys
... proximal tubular cells of human origin Therefore, human primary renal proximal tubular cells (HPTC) would be most suitable for such applications However, the application of primary human cells is ... effects on renal cells and possible applications will be discussed in detail 23 1.3 Genetic Engineering of HPTC and development of a BMP-7-producin...
Identification and characterization of proteins that interact with zonula occludens proteins
... IDENTIFICATION AND CHARACTERIZATION OF PROTEINS THAT INTERACT WITH ZONULA OCCLUDENS PROTEINS P JAYA KAUSALYA (B.Sc (Hons.), NUS) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY ... in TJ that are part of MAGUK family Fig 13: Structure of PDZ3 domain of PSD-95 with a peptide ligand Fig 14: Possible modes of interaction of PDZ containing protei...
Design, fabrication, and characterization of a solenoidsystem to generate magnetic field for an ECR proton source
... 2006 Acquisition and analysis of Langmuir probe characterization for ECR plasma Indian J Phys 80: 1011–1015 Jain S K, Jain A, Hannurkar P R, Kotaiah S 2007 Characterization of plasma parameter, ... distance for (a) mirror magnetic field, and (b) flat magnetic field the magnetic field can cause diffusion of the plasma particles to the wall of the plasma ch...
Identification and characterization of IFI30 as a glioblastoma specific promoter for glioma gene therapy
... astrocytoma grades I, II [astrocytoma], III [anaplastic astrocytoma] and IV [glioblastoma or GM]), oligodendrogliomas, ependymomas and mixed gliomas Gliomas constitute 77% of the primary malignant brain ... the characterization of a glioblastoma specific promoter This study deals with the identification, isolation and characterization of a glioblastoma specific...
Báo cáo sinh học: "Functional characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines" docx
... this article as: Lechner et al.: Functional characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse ... serum and regulated by association of a latency protein, precluded clear neutralization data Characterization of human CD33+ and CD1...
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