... Hegreness and Kishony http://genomebiology.com/2007/8/1/210
Genome Biology 2007, 8:201
Genome Biology 2007, 8:201
Minireview
Analysis of genetic systems using experimental evolution and
whole-genome ... Hegreness*
†
and Roy Kishony*
‡
Addresses: *Department of Systems Biology, Harvard Medical School, Longwood Avenue, Boston, MA 02115, USA,
†
Department of
Organismic...
... Genomewide analysis of mRNA
processing in yeast using splicing-specific microarrays.
Science 2002, 296:907-910.
22. Yeakley JM, Fan JB, Doucet D, Luo L, Wickham E, Ye Z, Chee MS, Fu
XD: Profiling alternative ... sample of 153 exon-skipping events predicted by the
microarray analysis, 73 were successfully validated by RT-
PCR and sequencing (a 48% validation rate). This initial
study h...
... pulmo-
nary fibrosis (IPF) is characterized by a predominance of
gene expression for Th2-type regulatory cytokines. This
pattern is associated with high levels of IL-4 and IL-5 and
a paucity of IFN-γ ... identical conditions and as previously
described [26,27].
Data Analysis
The genotype frequencies, allele carriage frequency and
allelic frequency were determined by direct count...
... therapy, focusing on a potential genetic clue to medication response.
Key words: asthma, genetic polymorphisms
O
ur understanding of the pathophysiology and treat-
ment of asthma is quickly advancing. ... majority
of studies indicate that the Gly16 SNP appears to carry the
greatest potential clinical impact. The wild genetic makeup
of the sixteenth amino acid position of the...
... Doyle A: Patterns of psychiatric comorbid-
ity, cognition, and psychosocial functioning in adults with
attention deficit hyperactivity disorder. Am J Psychiatry 1993,
150:1792-1798.
23. Ratey ... Consumer
and Specialty Pharmaceuticals.
Authors' contributions
JK and ML conceptualized and designed the study. ML
had primary responsibility for analysis of and interpreta-
tion...
... University.
Genetic analyses
DNA was extracted by the salting-out method described by
Miller and colleagues [18]. Analysis of genetic polymor-
phism was predominantly performed by polymerase chain
reaction ... previously
described [20].
Analysis of Fc
γ
RIIIa gene polymorphism
The analysis of the F/V polymorphism was performed
essentially as previously described [21].
MBL gene pol...
... analyses of the frequency of occurrence and
mutations of the highly mutable motifs RGYW and WRCY
revealed that mutations in nonproductive V
L
rearrange-
ments of B cells from the parotid gland ... nonproductive and productive repertoires of
these donors exhibited a comparable usage of Vκ and Jκ
as well as Vλ and Jλ gene elements [10,11].
Determination of Taq polymerase...
... cytotoxic granule and cytokine release), antigen presen-
tation and antibody-dependent cellular cytotoxicity [6]. Con-
versely, FcγRIIb contains an inhibitory motif in the cytoplasmic
tail and ... the collection
of clinical data and the recruitment of patients into the study.
DS, JR and VK undertook some of the genotyping assays on
DNA prepared in the laboratory of EAJ and...
... followed by 40 cycles of 95°C for
1 minute and 60°C for 15 seconds, as recommended by the
manufacturer (Applied Biosystems). Real-time data were ana-
lysed by using the Sequence Detection Systems software,
version ... 1
Standard curves generated for each assay by ten fold
serial dilutions of template,withreal-time data analysed by
using the Sequence Detection Systems software,...
... cultural examination of common bacteria and fungi, and of
direct acid-fast bacilli smears (Kinyoun method) and cultures
for mycobacteria, and for the microscopic examination of
Pneumocystis jiroveci ... of
pro-inflammatory cytokines, the influx of inflammatory cells,
and fibrinogenesis, whereas the downregulation of GSTP,
Cu–Zn SOD and cystatin SN could favor vascular...