Báo cáo y học: "Systematic analysis of transcribed loci in ENCODE regions using RACE sequencing reveals extensive transcription in the human genome" pptx
... have systematically analyzed the transcribed loci in
420 selected portions of the ENCyclopedia Of DNA Elements
(ENCODE) regions using 5' -RACE and 3' -RACE sequencing.
The ENCODE regions ... Biology 2008, 9:R3
Open Access
2008Wuet al.Volume 9, Issue 1, Article R3
Research
Systematic analysis of transcribed loci in ENCODE regions using
RAC...
... suggesting that if
the instance compound targets the instance protein,
inhibiting the heterozygous deletion strain, then co-
inhibiting compounds would be more likely to inhibit the
strain as ... absence of the target in
yeast. In the homozygous data, several drugs with no
obvious structural similarity clustered together (Figure
2c): rapamycin, calyculin A and wiskostatin....
... down-regulation in the autopsy
samples. Interestingly, we also see up-regulation of genes
involved in the ubiquitin cycle, protein ubiquitination, and
ubiquitin-dependent protein catabolism. This ... expression
patterns in postmortem human brains. Study of expression
levels of 14 genes in human brain autopsy and biopsy samples
found significant change in one of the...
... results in the formation of the branching epithelial tree
of the adult gland. The onset of puberty results not only in the
rapid growth of a ductal epithelial tree but also the appear-
ance of specialized, ... list).
Four out of five of the most highly upregulated transcripts
through the onset of puberty are members of the MUP family
of odorant-bindin...
... to identify key molecules in the course of hy-
poxia-induced apoptotic pathways. Such analysis of
trophoblastic cells will, in the near future, yield in-
sights into the mechanisms of preeclampsia ... proteins induced
by hypoxia in trophoblastic cells so as to clarify the mechanism of hypoxia-induced apoptosis by using the
PoweBlot, an antibody-based Western a...
... Biochemistry of the Charité, Berlin,
Germany using the ABI PRISM Big Dye Terminator
system (Applied Biosystems, Germany). Sequences
were analyzed using the Chromas software (Technely-
sium Pty Ltd, ... confirmed by sequencing DNA samples
of 51 healthy individuals. Interestingly, this SNP had
not yet been registered in any SNP databases despite
of its high frequency of...
... immunoglobulin
chains were also found to be significantly increased in
aged thymocytes. Understanding the changes in gene
expression in thymocytes with age may hold the key
in determining thymocyte ...
levels of many of these genes identified in the array
analysis. Moreover, Western blot analysis of thymo-
cyte lysates also confirmed that IgM increases with
age (...
... DM64 binds to the myotoxins through a myotoxic/
cytolytic site distinct from the catalytic site, as already
described for the inhibition of myotoxicity by heparin [44].
At least in the case of B. ... sequences. The conserved aromatic residues phenylalanine and tyrosine typical of the Ig-fold are bold in each domain.
Fig. 5. Inhibition of in vivo myotoxicity of myot...
... protein in the gel lane from
which the density measurement was made.
Centrifugation of p26 in sucrose gradients
Sucrose gradients were formed in 0.1
M
Tris/glycine
(pH 7.4) by layering 5 mL of ... signifi-
canceofasmallheatshock/a-crystallin protein (p26) in encysted
embryos of the brine s hrimp, Artemia franciscana. Am. Zool. 39,
836–847.
49. Liang, P. & MacRae, T.H. (...
... in ammogenic
activity of the intact molecule [6]. The presence of longer
(compared to the highly endotoxic lipid of E. coli)chain
fatty acids, the absence of phosphates and of an acyl group
at O-3 of a-GlcN ... (C)
p.p.m. can only be explained as resulting from the acylation
of A3withacyl
3
. O-Acylation of GlcN A at O-3 and of acyl
2
at O-3 agreed with the low-...