Báo cáo y học: " In silico modeling indicates the development of HIV-1 resistance to multiple shRNA gene therapy differs to standard antiretroviral therapy" docx

... 14RESEARC H Open Access In silico modeling indicates the development of HIV-1 resistance to multiple shRNA gene therapy differs to standard antiretroviral therapy Tanya Lynn Applegate1,2*, Donald ... directly to the development of an entire CD4+ Tcell population containing the therapeutic gene( s). It willlikely be contained within a minority of these cells.Hence gene therapy provides a very ... ar-bouring a reduction in fitness will not survive in the presence of an adequate gene therapy. The dynamics of virus outgrowth will be determine d by i) efficacy of the gene therapeutic inhibiting...

Báo cáo y học: "In silico experimentation with a model of hepatic mitochondrial folate metabolism" pps

... by the mitochondria iscut in half. Thus the elimination of mSHMT and externalglycine together is clearly very detrimental to the cell.Interestingly, the net flux of glycine remains from cytosol to ... being supplied by the mitochondriaso the rates of thymidylate synthesis and purine synthesisare greatly reduced. The rate of the cytosolic SHMTincreases approximately 18-fold and the cytosolic ... mitochondrial glycine, but the net flux remains from cytosol to mitochondria. On the other, if mSHMT is normal and we set the external glycine to zero, the concentrations of glycine in the cytosol...

Báo cáo y học: " In-Silico docking of HIV-1 integrase inhibitors reveals a novel drug type acting on an enzyme/DNA reaction intermediate" pdf

... [10,11]. IN inhibitors currently in clinical trials belong to the INSTI group. Chemically,they display a β-hydroxy carbonyl (Fig. 2), thought to bind the (possibly) two metal ions coordinating the ... transfer inhibitors (INSTIs) maps to the putative acceptor DNA binding sitePrevious studies showing a dependence of the inhibitoryactivity of INSTIs from the concentration of acceptor DNAled to the ... the 3D coordinates of the correspondingmetal in the aligned RSV IN. Molecular docking The 3D structures of well characterized IN inhibitorsincluding INSTIs in clinical trials were initially...

Báo cáo y học: "In silico evidence for the species-specific conservation of mosquito retroposons: implications as a molecular biomarker" ppt

... Structurally, introns are a six-moiety complex. Depending on the function of the proteinencoded within the IV moiety, the highly mobile class of group II introns or retroposons is sub-divided intotwo: ... to human spliceosomal elements To define the exact relationship of the hits obtained byquerying the C. pipien and An. sinensis retroposons against the human genome, theoretical functional inference ... pathogens, mosquito-targeted insecticidespraying predominates [6]. Nevertheless, control of the mosquito vector through insecticide spraying has proveddifficult in the past. In particular: (i)...

Báo cáo y học: " In silico analysis of chimeric espA, eae and tir fragments of Escherichia coli O157:H7 for oral immunogenic applications" doc

... prospec-tive engineering and re-engineering of protein therapeu-tics, reducing the risk of undesired immunogenecity andimproving the likelihood of success in clinical use.Finally, the conclusions ... SacI) were introduced at the ends of the syn-thetic gene for future cloning into plant expression vec-tors.Graphical depiction of the predicted minimum freeenergy for the synthetic gene showed ... secondary structure pre-diction was combined with comparative sequence analy-sis to determine the potential folding of the chimeric gene. The 5' terminus of the gene was folded in the waytypical...

Báo cáo y học: "Smoking and nicotine exposure delay development of collagen-induced arthritis in mice" pptx

... time-dependent immune-modulating effect of the sympa-thetic nervous system conferred by altering the cytokineprofile in the local lymph nodes and spleen of mice with typeII collagen-induced arthritis. ... (Figure2d).Ameliorating effect of cigarette smoke exposure on antibody productionTaking into consideration the role of antibody production in the pathophysiology of CIA, to determine whether cigarette ... nicotine on the production of pro-inflammatory cytokines, LPS (10 μg/ml) was used to stimulatenative NMRI splenocytes. As shown in Figure 4, cells incu-bated in the presence of nicotine with...

Báo cáo y học: " Protocol for a randomised controlled trial of risk screening and early intervention comparing childand family-focused cognitive-behavioural therapy for PTSD in children following accidental injury" pot

... trialexamining the efficacy of a tra uma focused, C BT earlyintervention for the treatment of PTSD in childrenfollowing exposure to accidental injury. Further, a con-trolled trial examining the ... improvements in psycho logical symptoms as well as health-related quality of life. Further, the design of the study will allow for anexamination of improvements and maintenance of gains(for the active ... unanswered,including the importance of parental involvement in treat-ment and optimal timing of interventions. The role of parents in the treatment of PTSD in childrenand adolescents The role of parenting...

Báo cáo y học: " Protocol for a randomised controlled trial of risk screening and early intervention comparing childand family-focused cognitive-behavioural therapy for PTSD in children following accidental injury" pptx

... controlledtrial of risk screening and early intervention comparing child- andfamily-focused cognitive-behavioural therapy for PTSD in childrenfollowing accidental injury. BMC Psychiatry 2011 11:15.Submit ... CH: Factors affecting the diagnosis and prediction of PTSD symptomatology in children andadolescents. American Journal of Psychiatry 2006, 163:644-651.3. Scheeringa MS, Zeanah CH, Myers L, ... screening and early interventioncomparing child- and family-focused cognitive-behavioural therapy forPTSD in children following accidental injury. BMC Psychiatry 2010, 10:92.2. Scheeringa MS, Wright...

Báo cáo y học: " In vitro bioassay as a predictor of in vivo response" pdf

... on [2]. But engineeredmodifications of hormones, growth factors or their ana-logs are likely to differ from the native analogues in theiraffinity for binding proteins. In view of this, an importantpractical ... define β (degree of conjugation) as the proportion of BAS that is free of binding proteins and is available to interact with cognate receptors. The larger is β, the larger the proportion of "free" ... equation (8) as the incoming signal, substitutedthis into equations (6) and (7) and solved analyticallyusing Math Cad 8 graphing software (MathSoft Inc., Cam-bridge, MA, USA) to predict in vivo responses...

Báo cáo y học: "Predictive network modeling of the highresolution dynamic plant transcriptome in response to nitrate" pps

... 4b). Interestingly, SPL9 seems to have aneffect on the vast majority of the genes in the reg ula-tory network that we have tested (Additional file 5). The diversity of the misregulation of gene ... processes dynamical models with nonlineardynamics to infer the profile of a single transcription factor (the tumor suppressor p53) and explained the activity of alarge c ollection of genes using that ... tran-scription factor in the control of genes involved in the NO3-primary response. We also further investi-gated the role of SPL9 over-expression on the tr an-scription levels of genes in the network...

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