... C-terminal sequence of the coding region: 5¢TTAC
AAGGACAAATTAATTGTGCCAG. For amplification
of the long isoform the same 5¢ primers were used, the 3¢
specific primer was FF3B: 5¢TTACAAGTCTTGCAA
AGGGAAGGAT. ... molecular mass protein allergens in
tomato extract (not shown).
Peptide map and glycan analysis of the natural
b-fructofuranosidase
Investigation of the carbohydr...
... intermediate
of 28 kDa (lane 4). PNGase F treatment of the
18 kDa subunit resulted in a partial shift towards a
lower molecular mass of 16 kDa (lane 4). Consider-
ing an apparent molecular mass of about ... using
the Scpep1 antiserum to detect the 35 kDa fragment and an anti-
Ctsa rat IgG2B to detect the 32 kDa heavy chain of Ctsa.
K. Kollmann et al. Functional...
... 5¢-CCTGATCGACGCACGAGT-3¢ and GT1-R,
5¢-TTTTGCAAGTCATAGTAATCAGTTT-3¢ for GT-
cDNA1 (2150 bp); and GT2-F, 5¢-CACCAGCAACTAC
CTGATCGA-3¢ and GT2-R, 5¢-CACAAAATATGCTT
CCAAGTGC-3¢ for GT-cDNA2 (3043 bp).
Construction and ... A, 5¢-TGTC
AGTCCTGTACAAAGAC-3¢ and Primer B, 5¢-CAT
CAGGCTTCCCCATA-3¢. Gene-specific nested primers
for 3¢-RACE were: Primer C, 5¢-CCAGTGTCCCCAT
CATCAG-3¢ and Prime...
...
Institutes of Health Research, DJC is a Research Chair of the Canadian
Institutes for Health Research, MAC holds a Career Investigator Award
from the Heart and Stroke Foundation of Canada, and PJD ... Regional Medical Associates
of McMaster University, Canada, and the Father Sean O'Sullivan
Research Center of St. Joseph's Hospital in Hamilton. We thank...
... running from midbrain to
hindbrain along the bilateral symmetry axis. The labe-
led specialized large and elongated cells of the anter-
ior part of the floor-plate were positioned at the base
of the ... cranial ganglia including the trigeminal
ganglia and their projections (Fig. 4A, B,D,E). The
hybridization signal was maintained in the ganglia up
to the larval...
... 5¢-GACGACGACA
AGATGGAGGTTAAGGATGAGTTG-3¢ and 5¢-GAG
GAGAAGCCCGGTCTATAACTCATCTTTGAGTAC-3¢
for GmPDIL- 3a, and 5¢ -GACGACGACAAGATGGAGG
TTGAGGATGAGTTGG-3¢ and 5¢-GAGGAGAAGCCCG
GTTCATAACTCATCTTTGACGAC-3¢ ... with a Thermal Cycler Dice Real
Time System (TaKaRa Bio Inc.). Forward primers 5¢-CG
TTTGAAGGGTGAGGAGGAAAA[FAM]G-3¢ and 5¢-CA
CAAGAGAGTTCTGCGATAACCTTG[FAM]G-3¢ (Invi-
trogen Corpora...