... for citation purposes)
BMC Plant Biology
Open Access
Research article
Ascorbate metabolism and the developmental demand for tartaric
and oxalic acids in ripening grape berries
Vanessa J Melino
1,3
, ... insights into Asc metabolism in wine grapes (Vitis vinifera c.v. Shiraz.) and thus ascertain
whether the developmental demand for TA and OA synthe...
... probably induced by streng-
thening of the interaction between the structural TnC-
binding site and the C-domain of TnC accompanying
Ca
2+
binding to site IV of TnC. In vertebrate tropo-
nin, the ... 116–
131). In the relaxed state, the inhibitory region binds
to actin and inhibits actin–myosin interaction [11,12],
while in the contractile state, Ca
2+
-binding to site...
... [18].
The biochemical basis of the gastrin–transferrin inter-
action is still unknown. Knowledge of the regions of
transferrin required for the binding of gastrin, and of
the regions in gastrin ... explore
whether Ggly interacts with transferrin in vitro,to
investigate whether iron is required for the Ggly–trans-
ferrin interaction, to define the domains ⁄ residues of
G...
... (Clontech)
were transformed with the combination of
plasmids shown. The growth of single yeast
colonies containing these plasmids are
shown in (B) and (E) on SD lacking
L-histi-
dine and adenine and (C) on ... were transformed with
the combination of plasmids shown. The
growth of single yeast colonies containing
these plasmids are shown in (B) and (E) on
SD plus
L-histidin...
... provides the
a-mating factor signal for secretion of the recombinant
protein.
Transformation and selection of the productive clones
Prior to the transformation the vector was linearized with
SacI. The ... proteolytic
activation; substrate specificity; protein inhibitor.
The understanding of the digestive process in pest insects
is a key step in the design of many inse...
... DIMs
in the cell envelope raises the possibility that these
compounds act in vivo by interacting with some com-
ponent of the host cell and by modulating the host
immune response to contain the infection. ... postinfection, we observed a
marked difference between the number of CFUs of
the wild-type strain and that of the PMM56 mutant
strain in lungs and in the...
... modulated during both
early and late responses.
We also examined the expression of TRAF1 and TRAF2
because they interact and regulate the function of the 4-1BB
proteins to induce NF-jB [18]. In the absence ... 4-1BB-Ligand, it increased the
caspase activities. An increase in the caspase activities was
also observed in the HeLaTR cells transfected with 4-1BB
cDNA, an...
... expressed in
Escherichia coli and used in pull-down assays. In these
experiments, cardosin A binds consistently to the C2
domain (Fig. 5, lane 2), indicating therefore that this
domain of PLDa ... sequences present in
integrin-binding molecules because of its exposed loca-
tion. On the basis of these structural findings, it was
hypothesized that the interaction between PLDa an...
... obtained in a few years. The described study proto-
col and the documentation forms are open for the use by
other study groups. To further increase the number of
patients in order to obtain faster ... drafted the manu-
script. AS, HP, CR, BP, BT, RDK and KD participated in the
design of the registry study and are involved in continuing
optimization. NW is the s...
...
an
increase
in
the
F
allele
frequency
in
autumn
and
winter
months
(fig.
2).
Total
monthly
rainfall,
mean
daily
maximum
and
minimum
temperature
for
each
month
and
the ...
underlying
the
large
scale
latitudinal
cline
in
Australasia.
The
negative
temperature
association
we
observed
is
therefore
in
the
opposite
direc-
tion.
Also,
in
t...