... reactions. The first round of PCR
reaction was performed with a forward primer UPM (a
mixture of primers 5¢-CTAATACGACTCACTATAGGGC
AAGCAGTGGTAACAACGCAGAGT-3¢ and 5¢-CTAAT
ACGACTCACTATAGGGC-3¢) and ... PCR
reaction was performed using a pair of specific primers
P3 (5¢-GGAAGAATACAGACACGGACAG-3¢) and P4
(5¢-ATAACAACAGTTTATACATCGCTTC-3¢) correspon-
ding to the 5¢-untranslated...
... 1297
Cloning, characterization and localization of a novel basic
peroxidase gene from Catharanthus roseus
Santosh Kumar, Ajaswrata Dutta, Alok K. Sinha and Jayanti Sen
National Centre for Plant Genome ... Skoog (MS)
3
basal medium by painting on
the adaxial surface of the leaves, and the tray containing
the leaves was sealed with saran wrap. In control experi-
ments, s...
... lectin on SDS/PAGE gave a single
band at apparent molecular mass of 34 kDa.
The binding affinity of the lectin in the hemolymph of
the freshwater crab, Paratelphusa jacquemontii, expressed
O-acetyl ... showing a( 2–6) linkage.
The sialic acid affinity of the Paratelphusa lectin was
further proved by its inability to inhibit sialidase-treated
BSM and bovine thyroglob...
... behavior is
typical of integral membrane proteins. The polypeptide with an
apparent molecular mass of 53 kDa appears as a double band in
unboiled samples (lanes A1 and B1).
Table 1. N-Terminal ... Materials and methods. (A) Data points
correspondtotheamplitudeofthetroughcenteredatg ¼ 1.951; as in
the low po tential range, the radical signal of the dyes overlap in t...
... binding of SmNR1(Ile247-Ser372) and SmRXR1(Glu251-Asn376) in vitro. DNA binding of a protein containing 20 amino acids at
the 5¢ end of the DBD, the DBD and the 40 amino acids at the 3¢ end of the ... of
A
DNA binding domain
B Ligand binding domain
Fig. 1. Sequence alignment. (A) Alignment
of DNA binding domain (C domain) and its
C-terminal extension. (B)...
... pull-down and
immunoblotting against anti-HA serum. Coomassie Brilliant Blue staining and anti-HA blotting of the input materials ascertain the equivalent
amounts of extracts and peptides between each ... transcriptional
activation (amino acids 1–63), N-terminal Elf-1
(amino acids 63–244), the basic (amino acids 244–
250), SPXX (amino acids 250–403), Q ⁄ P (amino acids
403–413...
... GSTs in insects [2]. The majority of GSTs
are in the Delta and Epsilon classes, and the remain-
ing enzymes are in the Omega, Sigma, Theta and Zeta
classes.
The German cockroach (Blattella germanica)isan
economically ... Point
Richmond, CA, USA).
Amino acid sequencing
Edman degradation, performed by Proteos, Inc. (Kalama-
zoo, MI, USA), was used to determine the am...
... 2).
Activation of class I PI3K inhibits autophagy
through activation of protein kinase B (Akt) and
mammalian target of rapamycin (mTOR). In contrast,
activation of class III PI3K in a complex with the
autophagy-associated ... 1-depen-
dent autophagy in yeast and mammalian cells [4].
Beclin 1, the mammalian ortholog of yeast
Atg6 ⁄ Vps30, was originally discovered in...
... 2957
Identification and characterization of a collagen-induced
platelet aggregation inhibitor, triplatin, from salivary
glands of the assassin bug, Triatoma infestans
Akihiro Morita
1
, Haruhiko Isawa
2
, ... or absence of 1.0 l
M triplatin-1 and analyzed by anti-FcR c-chain
(aFcR c-chain) and antiphosphotyrosine (aPY) immunoblotting.
A. Morita et al. Identification and chara...