... number not for citation purposes)
Journal of Nanobiotechnology
Open Access
Short Communication
A novel method of cultivating cardiac myocytes in agarose
microchamber chips for studying cell synchronization
Kensuke ... on-chip single-
cell sorting method for cultivating particular cells chosen
from clued mixture of cells [7], and have found the adap-
tat...
... found in the intercellular
space between mesophyll cells. The few haustoria that
were observed were small and – like haustoria of P.
infestans – digit-like in shape. In late stages of infection,
hyphae ... Protein (GFP) tagged
P. brassicae transformant. Microscopical examination of
the infected tissues showed hyphal growth in leaves,
stems and roots (Figure 1B, D, and 1F). In...
... points using forward linear prediction extra-
polation [28,29].
MALDI-TOF analysis
MALDI mass spectra were carried out in the negative
polarity in linear or in reflector mode on a Voyager STR
instrument ... were measured in the
1
H-detected mode via single
quantum coherence with proton decoupling in the
13
C
domain, using data sets of 2048 · 512 points, and 64 scans
were acquired...
... reduced cofactor in the
enzyme–glutamate–reduced cofactor complexes. In the
absence of glutamate zinc appears to significantly
tighten NADPH binding. Interestingly in the presence
of norvaline zinc ... 2.
Effects of zinc on cofactor binding
The effects of zinc on the binding of the reduced co-
factor NADPH to the enzyme, at pH 8.0, in the pres-
ence or absence of glutamate...
... (num-
bering in red) of the 11 residues surrounding ATB107 within 5 A
˚
in
mIGPS (A) were also included in the 14 residues surrounding sub-
strate within 5 A
˚
(B). (C) Binding regions of substrate ... the inhibition
mechanism of ATB107.
To investigate the role of these residues in the inhib-
itory effect of ATB107, we compared the binding sites
of CdRP and of ATB107. Th...
... 100 100
Minus pantoate 2
Minus ATP 46 50
Minus ATP, minus pantoate 3
Minus ATP, minus P
i
45
Minus ATP, minus ADP 3
Eqn (1)
Complete system 20
Minus pantoate 1
Minus ATP ND
Minus ATP, minus pantoate ... different
protein folds: the hexokinase, ribokinase, and galacto-
kinase families of sugar kinases. Protein Sci
2, 31–40.
18 So
¨
ding J (2005) Protein homology detection by HMM–
HMM compari...
... inhibitor of transcription actinomycin D,
and changes in the actin cytoskeleton were monitored
by fluorescence using rhodamine phalloidin staining. As
shown in Fig. 1A, TGFb caused potent actin ... organization of the actin cyto-
skeleton, resulting in cell flattening and cell shape
change. Cells appeared elongated or spindle-shaped,
with a parallel arrangement of actin bundles...
... (1993)
The linkage between binding of the C-terminal domain
of hirudin and amidase activity in human alpha-throm-
bin. Biochem J 289, 475–480.
45 Baglia FA & Walsh PN (1996) A binding site for
thrombin ... thrombin in absence of dextran
sulfate. Activation of both proteins by human
a-thrombin followed pseudo-first-order kinetics, as
shown in Fig. 3. This was in agreement...
... mechanisms for
maximizing the amount of information that can he
expressed within a limited time and space. These
mechanisms include increased frequency of complex noun
sequences and also increased ... Sager. Analysis and Pro-
cussing of Compact Text. Proc. COLING 82, 201-206,
North Holland.
[Marsh 1083] Marsh, E. Utilizing Domain-Specific Infor-
mation for Processing Compact...