báo cáo khoa học: "Energie métabolisable et digestibilité azotée chez les poulets en croissance : (1) influence du génotype, de l’âge et du régime" ppt
... comparing the importin-a:CLIC4 NLS
experimental electron density with density calculated
from the model. The CLIC4 NLS main chain fits the
density well, with an average RSCC of 0.96. Side
chains have greater ... difference density peak near the Tyr205 O–C
f
bond at the 9.1r map level, the strongest density peak
in the F
clic4nls
o
ÀF
apo
o
difference map. The presence of
Tyr205 is de nitive e...
... colorectal cancer detection and diagnosis or as
potential therapeutic targets. VDAC1, annexin A2 and
Keratin 8 variant have been identified [20]. Madoz-
Gurpide et al. tested seven potential markers ... protein
identification confidence interval of > 95% were confi-
dently assigned. When unique peptides were identified to
multiple members of a protein family, proteins with the
highest sequenc...
... zymogen is activated by a
GRASP55-dependent furin-dependent mechanism, with
the bulk of activation being GRASP55-independent.
The interaction observed between GRASP55 and PC5/
6B or PC7 together ... Protein concentration
was determined using a BCA protein assay kit (Perbio Sci-
ence UK Ltd, Cramlington, UK). The western blot presented
is a representative of three independent experiments.
Pro-...
... KLF4 is decreased in intestinal adeno-
mas of multiple intestinal neoplasia mice and in colo-
nic adenomas of familial adenomatous polyposis
patients. In this investigation, we first determined ... against the devel-
opment of atherosclerosis. By using the matinspector
Professional program (http://www.genomatix .de) and
the Transcription Element Search System (TESS;
http://www.cbil.upenn.edu),...
... rearrange
the pro-peptide. Generally, however, displacement of
the pro-peptide cannot be excluded although in cova-
lent peptide linkage, as the peptide region between the
pro-peptide and the mature ... S1). Similarly, using proBMP-2 under
identical assay conditions, only a low AP signal increase
and no concentration dependence was recorded
(Fig. 1B). Even when cells were stimulated with 1 lm...
... of
Shh-N; when palmitoylated, the N-terminal portion
would retain activity, even under denaturing condi-
tions. To understand, in full, the 5E1 epitope under
denaturing conditions, extensive analyses ... in the same blot.
Values were the mean ± SD of three
independent experiments. The difference
between total Shh-Np and modified Shh-Np
was determined using a paired t-test.
*, P < 0.01; NS,...
... nicotinamide to
move into a productive position [21]. This exchange
between Y308 and the NADP
+
nicotinamide has been
experimentally indicated as the enzyme rate-limiting
step [18]. Evidence has recently ... arrange-
ment of lumiflavin and phenol with the exact geometry
found between flavin and Y308 in the crystal structure
of pea FNR was used for the initial set-up. Then, dif-
ferent arrangeme...
... Pseudomonas fluores-
cens ACMSD revealed that the enzyme is metal-
dependent and catalyzes a novel type of nonoxidative
decarboxylation [21,33–35].
The human gene has been identified upon expression
in ... quantita-
ted as described in Experimental procedures, using the prim-
ers ⁄ probe sets reported in Table 1. Data are the mean of three
independent experiments and are presented as copies of...
... paralogue genes
(e.g. promoters, enhancers) which lead to temperature-
dependent mRNA levels have to be identified. Also,
for a more detailed understanding of the functional
and metabolic consequences, ... kJÆmol
)1
[20,21]
Pattern of heat denaturation (scanning
rate ¼ 2 °CÆmin
)1
between 10 and 110 °C)
There are three independent transition states during denaturation: tetramer fi
monomer fi dom...
... ROS dependent. Taking into account that NAC did
not reduce the size of inclusion bodies, the difference
in protection mediated by Hsps and NAC suggests a
correlation between the ROS-dependent ... disease gene: evidence against simple inac-
tivation due to an expanded CAG repeat. Somat Cell
Mol Genet 20, 27–38.
6 Petersen A, Mani K & Brundin P (1999) Recent
advances on the pathogenesis ......