... bind its natural ligand. For the host protein tofold it must be able to bring the N- and C-termini of theloop together. Thus, folding of the insert protein in such away as to bring its N- and ... solution structure of animmunoglobulin light chain-binding domain of protein L. Com-parison with the IgG-binding domains of protein G. Biochemistry33, 14011–14017.5. Kastern, W., Sjobring, U. & ... usingonly glycine, glutamate, leucine and lysine; arginine and proline were needed in the loops [16,17]. Hecht’s labshowed that generation of four helix bundles waspossible using only 11 of...