Báo cáo y học: "Elevated expression of caspase-3 inhibitors, survivin and xIAP correlates with low levels of apoptosis in active rheumatoid synovium" potx
... design of the study,
interpretation of data and preparation of the manuscript.
Figure 4
Expression levels of caspase-3, survivin and xIAP mRNAs in inactive RA, active RA and OA patientsExpression levels ... between survivin and cleaved caspase-3 expression.
Additionally, our finding that the mRNAs of caspase-3, xIAP
and survivin were generally hig...
... cohort study,
the Altrincham Pain Study, that psychosocial factors, including
reporting other non-pain somatic symptoms, aspects of illness
behaviour and high levels of psychological distress and
fatigue, ... accuracy of the findings. More importantly, subjects with
chronic widespread pain, when compared to pain free con-
trols, have increased rates of other symptoms, includin...
... played by cytokines in the evolution of
insulin resistance. Could hyperhomocysateineamia be a result of derangement of certain cytokine
production?
Abbreviations
tHcy: total homocysteine; ...
homocysteine levels in subjects with hyperinsulinemia. J Intern Med. 2000;247(2):287-94.
66. Fonseca VA, Fink LM, Kern PA. Insulin sensitivity and plasma homocysteine concentrations...
... expression profiling of human
erythrocyte could be an important key for under-
standing the machinery of anucleate protein synthesis
and its meaning in the pathophysiology of diseases.
Acknowledgements ... profiling
of human platelets using microarray and serial analysis of gene
expression. Blood 2003;101:2285-2293.
16. Weyrich AS, Lindemann S, Tolley ND et al. Change i...
... bridges. Type 3 cystatins, the
kininogens, are larger proteins containing three tandemly
repeated type 2-like cystatin domains. Type 2 cystatins
identified in higher animals include cystatins C, ... suggested function of
cystatin F is hypothetical. Although most cystatins inhi-
biting cathepsin L also inhibit cathepsin S, an inhibitory
activity of cystatin F against cathepsin S has not y...
... (1997) The crystal structures of Sinapis alba myro-
sinase and a covalent glycosyl±enzyme intermediate provide
insights into the substrate recogn ition and active- site machinery of
an S-glycosidase. ... 2002
20 lL of the enzyme/inhibitor mixture to 180 lLof
substrate (4NPGlc), incubating for 30 min at 37 °C, and
measuring the rel ease of 4NP.
Protein assays and protein seque...
... ma intained by two s ystems. One is de novo
synthesis from b uilding blocks, glutamate, cysteine, and
glycine, via two ATP-con suming s teps involving c-g lut-
amylcysteine synthetase ( cGCS) and ... meas-
uring its enzymatic activity and examining the gene
expression and localization of the protein. Levels of GR
activity, the protein, and the corresponding mRNA were
the hig...
... cells,
which is often a goal in recombinant protein expression.
MATERIAL AND METHODS
Vectors and cloning of genes
Construction of the vector family pLNO and its use for
transfection and expression of immunoglobulin ... major
immunoglobulin of external secretions and is composed of
four different polypeptide chains produced by two distinct
cells. Polymeric IgA (pIgA)...
... analysis
of the interaction between CCT and b-tubulin by cryoelec-
tron microscopy allowed the identification of three regions
from the N-terminal, and five from the C-terminal domains
of b-tubulin, ... Gif-sur-Yvette, France
Mammalian tubulins and actins attain their native con-
formation following interactions with CCT (the cytosolic
chaperonin containing t-complex polypeptide 1...
... product cathenamine and involves 4,21-dehydrocory-
nantheine aldehyde as an intermediate. The enzymatic
hydrolysis of dolichantoside (Nb-methylstrictosidine) leads
to several products. One of them was ... condensation of tryptamine, the
decarboxylation product of tryptophan, and the monoter-
pene secologanin catalysed by the enzyme strictosidine
synthase (SS) [11]. The biosyntheti...