Báo cáo khoa học: "Comparison of cardiac function and coronary angiography between conventional pigs and micropigs as measured by multidetector row computed tomography" docx
... lLof50mm potassium phosphate buffer
containing 1 mm EDTA-K
2
(pH 7.4), and the oxidation of
NADPH was spectrophotometrically measured for 3 min at
340 nm [74]. The activity of GPx and GRd was ... iNOS. Ten nanograms of RNA extracted from mouse heart
was used, and quantification of iNOS mRNA was performed by
real-time RT-PCR. The relative level was calculated as
the ratio o...
... system, as described in (B). The densi-
ties of the bands were determined and plotted as a function of the
amounts of LolB and mLolB. LolB and mLolB (each 1 lg) were
analysed by SDS–PAGE, followed by ... liposomes and mLolB. In contrast, Pal remained
soluble when either mLolB or liposomes were omitted.
The amount of Pal incorporated into liposomes was
determined and...
... constraints. As a
result, in this case, "passive" modification case frame is
selected as a best matching, and case role of each case
element is determined as follows :
casel= Object, case2 ... representations of
noun phrases or adverb phrases while the predicate has
that of verb or adjective or adjective verb. A noun
phrase is defined as the recursive concaten...
... MC was added
from an aqueous stock solution (10 mm). Incubations were
routinely performed at 30 °C.
Measurement of swelling and cytochrome c
release
Swelling of mitochondria was measured as decrease ... Ca
2+
concentration was measured
with CaG as Ca
2+
fluorochrome. Representative traces of a single
preparation out of three preparations are shown.
A
B
Fig. 4. Effect of MC...
... and the supernatant recovered
to give a final protein concentration of 2mgÆmL
)1
. Pro-
tein concentration was measured by Bradford Assay (Bio-
Rad), and radioactivity per lg protein was measured ... absence of Hsp90 function. As a conse-
quence of ansamycin pretreatment, the rate of Hsp
synthesis is maintained for a longer duration and
excess amounts of each of th...
... Gapped
BLAST and PSI-BLAST: a new generation of protein
database search programs. Nucleic Acids Res 25,
3389–3402.
23 Falk A, Ek B & Rask L (1995) Characterization of a
new myrosinase in Brassica ... been classified as a myrosinase-bind-
ing protein-like protein. Myrosinase is a glucosinolate-
degrading enzyme [4], and myrosinase-binding protein
(MyroBP) has been identified as a...
... transfection and luciferase activity
measured using the Dual-Luciferase Reporter Assay System
from Promega (Madison, WI, USA). Luciferase activity
was defined as the ratio of firefly luciferase activity ... by polymerase II promoters are
based on endogenous small RNAs ( 22 nucleotides)
known as microRNAs (miRNAs) that can also guide
cleavage of RNAs and ⁄ or translational inhibi...
... significance of the assembly of the
elementary unit of fibroin in ER
It was demonstrated in this study that the elementary
unit of fibroin, having the same molar ratio of H- and
L-chains, and fhx/P25 as of ... °Cfor1minandA
550
was measured. Fractions containing Golgi complex were
monitored by the assay for Golgi a-mannosidase II [13–15]
as follows. A 15-lL portion of ea...
... was produced [14]. This phenom-
enon was a lso d escribed for the Trametes villosa laccase
produced in A. oryzae [6]. Glycosylation was 0.5% of
the molecular mass of t he native laccase and and ... degrade the products of the cellulases [24,25].
Lignin degradation occurs through the action of oxidore-
ductases, such as manganese peroxidase (MnP), lignin
peroxidases (LiP) and lac...
... TMRM taken up by the cells as well
as the extent of the second phase of TMA-DPH fluo-
rescence quenching by TMRM (Figs 6 and 7). By con-
trast to over-expression of Pgp, over-expression of
MRP1 had ... addition of cells to a
medium containing TMA-DPH, the fluorescence of the
latter increased by at least a factor of 100 as a result
of adsorption of dye on the ou...