Báo cáo khoa học: "Altered expression of thioredoxin reducta

Báo cáo khoa học: "Altered expression of thioredoxin reductase-1 in dysplastic bile ducts and cholangiocarcinoma in a hamster model" pptx

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Ngày tải lên : 07/08/2014, 18:21

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Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx

... caused by iron loading has an impact in protein integrity, as indicated by an increase in protein-bound acrolein adducts at the cell surface, which point to acrolein-adducts as a reliable marker ... 5¢-GGGCACTC AGCCAGGGGACATCCTGCCCAA-3¢ as forward and 5¢-GATACAAGTTTGCACACCTTTGCACTTCTG-3¢ as reverse [58]. The PCR ampliﬁcation was performed in a total volume of 50 lL reaction mix...

Ngày tải lên : 19/02/2014, 16:20

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Báo cáo khoa học: Altered expression of tumor protein D52 regulates apoptosis and migration of prostate cancer cells potx

... data were analysed using winmdi software (The Scripps Research Institute, La Jolla, CA, USA). Determination of caspase-3 and -9 activity Caspase-3 and -9 activities were measured 48 h after down- regulation ... determined the involvement of caspase activation and mitochondria membrane depolarization. In many cell types, activation of procaspase-3 is a distinguishing feature...

Ngày tải lên : 30/03/2014, 02:20

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Báo cáo khoa học: Temporal expression of heat shock genes during cold stress and recovery from chill coma in adult Drosophila melanogaster pdf

... (forward) TGGATGAACCCACACCCAATC 89 Hsp67Ba (reverse) CGAGGCAACGGGCACTTC Hsp68 (forward) GAAGGCACTCAAGGACGCTAAAATG 88 Hsp68 (reverse) CTGAACCTTGGGAATACGAGTG Hsp70Aa (forward) TCGATGGTACTGACCAAGATGAAGG ... CTCCTCGTGCTTCCCCTCTACC Hsp40 (forward) GAGATCATCAAGCCCACCACAAC 112 Hsp40 (reverse) CGGGAAACTTAATGTCGAAGGAGAC Hsp60 (forward) ACATCTCGCCGTACTTCATCAACTC 66 Hsp60 (reverse) GGAGGAGGGCATCTTGGAACT...

Ngày tải lên : 06/03/2014, 09:22

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Báo cáo khoa học: Molecular imprinting of cyclodextrin glycosyltransferases from Paenibacillus sp. A11 and Bacillus macerans with c-cyclodextrin pptx

... Degree of derivatization of the CGTases from A1 1 and BM obtained with different protein ⁄ itaconic anhydride ratios and remaining cyclization activity. Ratio a (w ⁄ w) Paenibacillus sp. A1 1 Bacillus ... Sunnyvale, CA, USA) to analyze and quantify the CD products. A Carbo- pac PA-100 analytical column (4 · 250 mm; Dionex Corp.) was used. A sample (25 lL) was injected and...

Ngày tải lên : 07/03/2014, 10:20

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Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt

... (5¢-TTT TTC TAG AGC AGG GTA GGT TGA TTT CAT GTC GAA TG-3¢; additional XbaI site underlined) and OB 3 (5¢-AAA AGA ATT CTT AGA AGT CCC AGT CAT CGT C-3¢; additional EcoRI site underlined). The ampliﬁed ... nrdF + gene was sequenced by a primer walking approach. For DNA analysis, dnastar software (DNAS- TAR Inc., Madison, WI, USA) and clone manager 5.0 (Scientiﬁc & Educational Software,...

Ngày tải lên : 15/02/2014, 01:20

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Tài liệu Báo cáo khoa học: Differential expression of duplicated LDH-A genes during temperature acclimation of weatherﬁsh Misgurnus fossilis Functional consequences for the enzyme ppt

... forward TGTGAAACGCAGTCTCTTCC H1F 122 (with H1F and H1R) 12 reverse CAAGGAGCGTTAGAATCTAAAG H1R 13 long forward TCTCCAAACCAGATCTCTACAG H2F 224 (with H2F and H2R) 14 reverse GATTTAAGTGGAGCGGAATGCTA ... 8 both forward ACAACACCACTGCTGCGGAGTTA J1F 9 short reverse ACATCAAGGAGCGTTAGAATCTAA J2R 1201 (with J1F and J2R) 10 long reverse GATTTAAGTGGAGCGGAATGCTA J3R 1385 (with J1F and J3R) Real tim...

Ngày tải lên : 19/02/2014, 02:20

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Tài liệu Báo cáo khoa học: Enhanced expression of Mcm proteins in cancer cells derived from uterine cervix docx

... Coomassie Brilliant Blue staining. Table 2. Quantitation and comparison of proteins in GM and WI-38 cells. From the data in Fig. 2 and others, the concentrations of Mcm proteins, ORC2 and PCNA in ... chromatin-bound proteins (Fig. 3 and Table 2). In contrast, the amounts of total Fig. 4. Abundance of Mcm2 mRNA in HeLa and WI-38 cells. (A) Total mRNA was puriﬁed f...

Ngày tải lên : 20/02/2014, 23:20

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Báo cáo khoa học: Increased expression of c-Fos by extracellular signal-regulated kinase activation under sustained oxidative stress elicits BimEL upregulation and hepatocyte apoptosis pot

... Se, 5¢-GGGAACAGAGCGGAUAGGATT-3¢; scrambled c-Jun siRNA2 Se, 5¢-GAAAGAUGGCAGAAUAGAATT-3¢; and scrambled c-Jun siRNA3 Se, 5¢-GAAAGCCUUAAGAA UUGUATT-3¢). The transfection of rat primary hepatocytes with siRNA(s) was carried ... immunoprecipitates including DNA were analyzed by PCR (primers: Fw, 5¢-CCAGACAATCGTCTCGCCCA-3¢; and Rv, 5¢-GGCTAGGTAACAGTTTAGCGAGGA-3¢). Rat genomic DNA extracted f...

Ngày tải lên : 06/03/2014, 00:21

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Báo cáo khoa học: Functional expression of olfactory receptors in yeast and development of a bioassay for odorant screening docx

... using primers (5¢-CGTCAAGGAGAAAAAAC CCCGGATCTAAAA AATGGAGC AGAAA CTCATCTC TGAAGAGGATCTG -3¢) and (5¢- GCATGC CTGCAGG TCGACTCTAGAGGATCTCAAGCCAGTGACCGCCT CCC-3¢), and checked for the presence and ... (5¢-AG CTGCCTGCAGGTCGACTCTAGAGGATCCTAACCAA TTTTGCTGCC-3¢); for OR17-40 (5¢-CGTCAAGGAG AAAAAACCCCGGATCTAAAAAATGGAGCAGAAAC TCATCTCTGAAGAGGATCTG-3¢) and (5¢-GCATG CCTGCAGGTCGACTCTAGAGGATCTCAAG...

Ngày tải lên : 07/03/2014, 16:20

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