... 2010
Accepted 19 April 2010
Available online 27 April 2010
Keywords:
Polysaccharide structure
Antioxidant assay
Tricholoma matsutake
abstract
In this study, structural features of Tricholoma matsutake ... available at ScienceDirect
International Journal of Biological Macromolecules
journal homepage: www.elsevier.com/locate/ijbiomac
Structure elucidation and antioxidan...
... 3279
Physicochemical characterization and biological activity
of a glycoglycerolipid from
Mycoplasma fermentans
Klaus Brandenburg
1
, Frauke Wagner
1
, Mareike Mu¨ ller
1
, Holger Heine
1
,Jo¨ rg Andra¨
1
, ... i.e. agonistically as well
as antagonistically, completely inactive. The lack of
antagonistic activity may be explained by the fact that
this lipid A does not intercala...
... trypsin, and the
digests were analyzed by LC-MS ⁄ MS analysis as in [40–42].
Catalytic assay
For plasmid substrates, uracil-containing plasmid DNA
was prepared by amplification of normal plasmid DNA
(pSUPERIOR-puro; ... serum at 1 : 180 000 dilution as primary anti-
body, and peroxidase-labeled secondary antibody. Extracts
from D. melanogaster and T. castaneum larvae were pre-
pared...
... that lack a capsular structure, which in itself provides
serum resistance. Recent data from our laboratory indicate
that the ability of acapsular strains of H. influenzae to
elaborate sialylated ... Encapsulated strains can cause invasive,
bacteraemic infections such as septicemia and strains
lacking a capsule, so-called nontypeable strains (NTHi),
are a common cause of otitis...
... (Uppsala, Sweden), a ZORBAX
300SB-C18 semipreparative column was from Agilent Tech-
nologies (Santa Clara, CA, USA), and trifluoroacetic acid
and acetonitrile used for HPLC were from Merck (Darms-
tadt, ... of conotoxins
and their analogs [24–27].
Most NOESY crosspeaks were assigned and inte-
grated, with concomitant cycles of structure calcula-
tions for evaluation of di...
... CTGATCTAGAGGTACCGGATCC
5RACF ATCCTCACGAACAAGCAG
5RACR GATCGCGATGCAGGCCTT
FLF1 GGACGACTACAGCGTCTTCAGTAGA
FLR1 TCCAAACAGTCAGTTTCTTAACCGT
Table 1. Purification of cellulase from abalone Haliotis discus hannai. ... The translational start codon ATG, termination codon TAA, and a putative polyadenylation signal AATAAA are boxed. A
putative signal peptide is indicated by a dotted underlin...
... We also found reactivity of some sera
to a 9- and a 15-kDa band. We could show that the
9-kDa band in the tomato extracts reacts with a specific
antibody against the LTP from cherry, Pru av 3 and ... N-terminal sequence of the mature protein: 5¢TAT
GCGTGGTCCAATGCTATGC, and FF 3A, matching
with the C-terminal sequence of the coding region: 5¢TTAC
AAGGACAAATTAATTGTGCCAG. For...
... substrate, with the concentration of O
2
as electron acceptor held constant. Kinetic data were determined at 30 °C
using the standard ABTS assay and air saturation.
Variant
D-Glucose D-Galactose
K
m
(mM) ... system
(Peqlab, Erlangen, Germany) and using the molecular mass
standards High Molecular Weight Calibration Kit for
native electrophoresis (Amersham Pharmacia, Piscataway,
NJ, USA)...
... Castro-Vazquez A
(2005) Control of the seasonal arrest of copulation and
spawning in the apple snail Pomacea canaliculata
(Prosobranchia: Ampullariidae): differential effects of
food availability, water ... Effects
of submersion and aerial exposure on clutches and
hatchlings of Pomacea canaliculata (Gastropoda: Amp-
ullariidae). Am Malacol Bull 20, 55–63.
3 Albrecht EA, Koch...