... Journal
Open Access
Methodology
Discovery of herpesviruses in multi-infected primates using locked
nucleic acids (LNA) and a bigenic PCR approach
Sandra Prepens
1
, Karl-Anton Kreuzer
2
, Fabian ... of the second
round of the pan-herpes DPOL PCR. It overlaps with the
LNA-based and bigenic amplification of beta- and gammaherpesvirusesFigure 1
LNA-ba...
... Ashraf and Nirod Chandra Saha
Address: Health Systems and Infectious Diseases Division, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh
Email: Humayun Kabir* ... Sirajuddin AKM, Ashraf A: Impact of manage-
ment training on family planning and health services per-
formance in rural Bangladeh. J Int HRD 2000, 4:194-202.
13. Incorporation of commu...
... were located in Africa
(Chad, Côte d'Ivoire, Mozambique and Zimbabwe), one
in Asia (Sri Lanka), and one in the region of Latin America
and the Caribbean (Jamaica). As seen in Table 1, the coun-
tries ... having different
entrance criteria, curricula and durations of training, and
oversight regulations, then grouping them into categories
that are nationally and int...
... and maintenance of plant and machinery used to cultivate
cropland and maintain livestock housing, and indirectly, in the form of manufactured
mineral fertilizers and pesticides. The level of ... A distinction was made between ruminant
animals and monogastric animals. This calculation yielded the area of arable land and
grassland needed for animal feed production...
... experimental work (including the
preparation and characterizations of the sample and the analysis of experimental data). XY
participated in the analysis of the particle size, morphology, and PXRD ... results and the
drafting of the manuscript, and they read and approved the final version.
Authors' information
IY has a Ph.D. degree in Pharmaceutical Scienc...
... A1 0L (P 4a) gene was amplified by polymerase chain
reaction using oligonucleotides KH10 (5' CATGCCAT-
GGATGATGCCTATTAAGTCAATAGTTACT CTT-3') and
KH11 (5'-CCGCTCGAGTTATTCATCATCAAAAGAGACA-
GAGTC-3'), ... protease, and an ubiq-
uitin-degrading enzyme in yeast, as well as the identity of
a catalytic triad composed of histidine, cysteine, and
aspartic acid, I7L...
... overscores.
CGGGCGCACGCAGC
U
AG
U
G
U
GGCAGAGAC
U
A
U
GGCC
U
AC
UU
G
U
GGGACCAAAACCAAGCG
UU
G
UU
C
U
GG
UU
GGAG
UUU
GCGGCCCC
U
G
UU
GCC
U
GCA
U
CC
U
CA
U
CA
U
CACG
U
A
UU
GCC
U
C
AGAAACG
U
GC
U
G
U
G
UU
GC
U
G
U
AAGAGCC
UUU
C
UUUUUU
AG
U
GC
U
AC
U
GAGCC
U
CGGGGCAACCGCCAGAGC
UU
ACGAACA
UU
CGACAG
U
AA
U
GCCGAACG
U
GG
U
GGGG
UU
CCCG
U
A
U
AAG
RAHAASVAETMAYLWD
Q
N
Q
ALFWLEFAAPVACI L I I TYC...
... was determined by plotting ln (A
t
⁄ A
o
) (where A
o
and
A
t
denote the intensity of protein bands of intact unhydro-
lized protein at the beginning and at the fixed time of incu-
bation) against ... was incubated for
10 min at 37 °C and the reaction was started by rapid
heating up to 43 °C. Aggregation of insulin and rhodanase
was followed by measuring attenuance at D...