... 37 °C for 1 h and furt her incubated for 6 h , the cellular levels of Hsp105a and Hsc70 (a constitutive isoform of Hsp70)increased, and t he expression of Hsp70 (an inducibleisoform of Hsp70) ... applications. In this study, we examined whether NSAIDs generallyinduce the expression of hsps and the resistance of cellsusing a COX inhibitor IND and a LOX inhibitor NDGA, and revealed that ... mMdithiothreitol,0.42MNaCl and 25% (v/v) g lycerol], incubated at 4 °C for 15 min, and vortexed for 15 min at 4 °C. Aftercentrifugation at 50 000 g for 5 min, aliquots of thesupernatant (15 lg protein) were incubated...