... Effect of ibuprofen and warfarin on the allosteric properties of
haem human serum albumin
A spectroscopic study
Simona Baroni
1
, Marco Mattu
2
, Alessandro Vannini
2
, Rita Cipollone
2
, ... not appear to be
a likely candidate for the axial haem bonding.
CONCLUSIONS
The effect of ibuprofen and warfarin on the electronic
absorption spectrosc...
... (TGCCGAATTCTA
CCAGTGCCAGTGAAGGACATCTTTGCCCACGTTG
ACCC), 4 ( CAACGTCATAGACGATTACATTG CTAC
ATGGAGCTGTCTAGAGGATCCGA). A three-strand
junction was made by o mitting strand 4 and a 37-bp duplex
DNA by annealing ... junc tion, J0, labelled with biotin ( bio) at the 5¢ end of
one strand: 1 (bio-AAAAATGGGTCAACGTGGGCAA
AGATGTCCTAGCAATGTAATCGTCTATGACGTT),
2 ( GTCGGATCCTCTAGACAGCTCCATGTTCACTG...
... various charac-
terization methods: fractionation by size-exclusion and
anion-exchange HPLC, amino acid analysis, glycosami-
noglycan and calcium quantification, SDS/PAGE and
FTIR spectroscopy. ... to analyze
the amino acid composition of the water insoluble matrix
(WIM). Again we found a high content in Gly-Ala, and the
global composition was very similar to the WSM, and...
... Fourier-deconvoluted and the
secondary structure was determined by Gaussian curve-
fitting. The calculation of each fraction of the total band
area over the curve was performed with an overlap
method after ... presented an
intermediate degree of amyloid fibril formation.
Cysteine has the capacity to form disulfide bonds. As
disulfide bonding of the protein may participate in...
... Avda, Salamanca,
Spain;
2
Centro Hispano-Luso de Investigaciones Agrarias, Universidad de Salamanca, Edi®ci o Departamental, Avda, Salamanca,
Spain
The Phycomyces blakesleeanus wild-type is yellow, ... strain A4 86 complemented carA, carRA and carC
mutations. These observations allowed us to discard any
possible a lteration in genes carRA and carC in strain A4 86,
in agreement with...
... whereas the stimulation decreased at higher
concentrations. At 1 m
M
of S-decyl-glutathione, the meas-
ured ATPase activity represented approximately the basal
ATPase activity. This type of behaviour ... decanol did not show any
stimulation, and caused a small, but significant and
concentration-dependent inhibition of the ATPase activity.
The ATPase activity of MBP–NBD2...
... work was supported by grants from the Danish Medical
Research Council and the Novo Nordisk Foundation (AAJ, JLH, SPS
and HBO), by the Lundbeck Foundation (AAJ) and by the Danish
Heart Foundation ... GFP
2
-tagged CaRs and
AT
1a
Rs are similar.
To evaluate the cell surface expression, we tagged HA
and c-myc epitopes to the N-terminal of the CaR-GFP
2
and
CaR-Rluc...
... data were analyzed by Systat software (Sys-
tat Inc) and KaleidaGraph software. Variables were
presented as mean values ±SD. Statistical analysis was
done by linear regression model and paired ... syndrome [30]. All these abnormalities cre-
ate a state of constant and progressive damage to the
vascular wall, manifested by a low-grade progressive
inflammatory process and en...
... 2 analysis of variance (ANOVA). For the first data
analysis, treatment and time were the independent
factors using all 23 subjects. The second analysis sep-
arated and analyzed the data according ...
Abstract
Confusion and controversy exist regarding the cardiovascular effects of dietary supplements
containing caffeine and Citrus aurantium (bitter orange) extract....
... bp
[rA]
29
, [rA]
4
, and [rA]
1
as substrate. These oligomeric
substrates were prepared by hybridizing 1 lm of the
5¢-FAM-labeled 29 base DNA
13
-RNA
4
-DNA
12
(5¢-AATA
GAGAAAAAGaaaaAAGATGGCAAAG-3¢) and ... DNA
15
-
RNA
1
-DNA
13
(5¢-AATAGAGAAAAAGAAaAAAGATG
GCAAAG-3¢) with a 1.5 molar equivalent of the comple-
mentary DNA, respectively, as described previously [10]. In
these se...