Báo cáo Y học: Purification and characterization of the thyrotropin-releasing hormone (TRH)-degrading serum enzyme and its identification as a product of liver origin doc
... derivatives as indicated in the table. The molar masses and the absolute amounts of the inhibitors and the maltodextrins
added to the filter paper discs are indicated. The size of the zones of growth ... to about half the area of the total cross-section [6].
Removal of the globular domain may increase the flexibility
of loop 4 and other surface expose...
... they are also known as the E-NTPDases.
Ecto-ATP-diphosphohydrolase (ecto-ATPDases) and ecto-ATPases
are two different subfamilies of the E-ATPases. E-type ATPases are
ATPases that have similar ... Lewis-Carl,S.&Kirley,T.L.(1997)Immunolocalizationofthe
ecto-ATPase and ecto-apyrase (ATPDase) in chicken gizzard and
stomach: purification and N-terminal sequence of the stom...
... soybean b-amylase (Cys82, Cys97, Cys208 and Cys343).
On the analogy of the soybean b-amylase, the active
site of the C. sepium b-amylase most probably consists
of a cleft located between the barrel ... against b-amylase and
C. sepium
RNase-related protein (CalsepRRP)
Polyclonal antibodies were raised against b-amylase and the
unglycosylated isoform of CalsepRRP [14...
... neuroprotection;
quinolinic acid; tryptophan metabolism.
Rapid progress in the pathophysiology of human diseases
has always been hampered by the availability of human
tissue, aesthetics, and ethical considerations. The ... strong anionic and cationic sepa-
rations. The purity of the enzyme was determined by SDS/
PAGE, and the molecular mass verified by MALDI-TOF
MS....
... assay
After each chromatographic step the inhibitory activity of
collected fractions was determined using papain as the
enzyme in the assay [6]. The same assay was used to
determine inhibitory activites ... kininogen carries biantennary and
triantennary N-glycans that are terminated by a2 ,3-linked
sialic acids, a high number of which are O-acetylated. About
1/3 of the...
... buffer
containing 0.2
M galactose.
Protein and carbohydrate analysis
The concentration of protein was measured by the method of
Lowry et al. [13] using BSA as the standard. The presence of
sugar in the ... Purification and characterization of three galactose specific lectins
from Mulberry seeds (
Morus
sp.)
Tanzima Yeasmin, Md Abul Kashem Tang, Abdur Razzaque and N...
... polymerase
(Stratagene) was used to amplify vanXY
C
usingpAT70 4as
template with primers A (5¢-GCTA
GGTCTCAATGAAC
ACATTACAATT-3¢)andB(5¢-TATG
GAATTCTCATG
CGAACTGCCTCA-3¢) that included BsaIandEcoRI
restriction ... bi[tris(hydroxymethyl)-meth-
ylamino] propane and 10 lL10m
MD
-Ala-
D
-Ala, incuba-
ted at 37 °C for 30 min, and assayed for
D
,
D
-dipeptidase
activity using the
D
-amino...
... enzyme
required the adenosylcobalamin coenzyme for catalytic
activity and the K
m
for the cofactor was 8 l
M
. Inactivation
of the enzyme was observed by both glycerol and cyano-
cobalamin. The ... °C.
Dehydratase assays
Two methods were used for the determination of the
activity. During the purification and characterization
enzyme steps, the assay was car...
... SE of n ¼ 3 separate determinations.
Table 2. Effect of various divalent metal cations and EDTA on the
activity of SAP1 and SAP2. The standard assay A was used except that
the phosphoenolpyruvate ... the hydrolysis of 1 lmol of
substrate per min at 25 °C.
Phosphatase assay A
For routine measurements of AP activity, the hydrolysis of
phosphoenolpyruvate to pyruva...
... °C.
Synthesis of XAC-agarose gel
The ligand af®nity gel was prepared based on the method
described by Nakata [15]. XAC was dissolved in dimethyl-
sulfoxide at a con centration of 0 .5 mgámL
)1
.Af®-Gel10
resin ... N-terminal protein
sequencer (Procise 494, Applied Biosystems).
Protein assay and amino-acid analysis
Protein concentrations were determined by the Amido black
assay [...