Báo cáo khoa học: Role of the N- and C-terminal regions of t

Tài liệu Báo cáo khoa học: Role of K22 and R120 in the covalent binding of the antibiotic fosfomycin and the substrate-induced conformational change in UDP-N-acetylglucosamine enol pyruvyl transferase docx

... with the K22V and K22E mutant proteins. This was interpreted as a lack of fosfomycin binding to these mutant proteins [12]. Binding of fosfomycin to either wild-type MurA or the K22 mutant proteins ... of R120 by lysine reduces the probability of forming the fully closed form of the protein. The results obtained with the K22V/R120K double mutant protein point towa...

Ngày tải lên : 19/02/2014, 13:20

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Báo cáo khoa học: Role of the hinge peptide and the intersubunit interface in the swapping of N-termini in dimeric bovine seminal RNase pptx

... Role of the hinge peptide and the intersubunit interface in the swapping of N-termini in dimeric bovine seminal RNase Carmine Ercole 1 , Francesca Avitabile 1 , Pompea Del Vecchio 1 , Orlando ... result also provides indirect evidence of correct pairing of the inter- chain disulﬁde bridges in the recombinant sample [11]. The kinetics of the N-terminal arms sw...

Ngày tải lên : 16/03/2014, 23:20

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Báo cáo khoa học: Mycobacterium tuberculosis ClpC1 Characterization and role of the N-terminal domain in its function ppt

... additional N-domain, homologous to the N-domains of ClpA or ClpB, and a linker domain homologous to, but half the size of, the linker domain of ClpB [19]. The N-terminal region contains two 32-amino ... shock proteins ClpP1, ClpC1, ClpX and ClpC2 have been annotated. Bearing in mind the importance of the Clp family of proteins in survival and virulence, it is o...

Ngày tải lên : 23/03/2014, 06:20

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Báo cáo khoa học: Role of the N- and C-terminal regions of the PufX protein in the structural organization of the photosynthetic core complex of Rhodobacter sphaeroides pptx

... between the RC and the cyt bc 1 in the presence of mutated PufX protein The role of the PufX protein in facilitating the ubiquinone/ ubiquinol exchange between the Q B site of the RC and the ubiquinone ... Role of the N- and C-terminal regions of the PufX protein in the structural organization of the photosynthetic...

Ngày tải lên : 31/03/2014, 09:20

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Tài liệu Báo cáo khoa học: Role of the cag-pathogenicity island encoded type IV secretion system in Helicobacter pylori pathogenesis pptx

... control the amount of intracellu- lar CagA PY . Cortactin, ezrin and vinculin are all Src substrates, and Src inactivation causes dephosphoryla- tion of these proteins and is crucial for triggering ... actin-binding proteins [6]. The phosphatases involved in this scenario, however, remain unknown and do not require Shp2. Instead, CagA PY can inhibit Src activity both by dire...

Ngày tải lên : 14/02/2014, 19:20

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Tài liệu Báo cáo khoa học: A novel c-N-methylaminobutyrate demethylating oxidase involved in catabolism of the tobacco alkaloid nicotine by Arthrobacter nicotinovorans pAO1 ppt

... C-terminal domain of MABO to other proteins of the sarcosine dehydrogen ase and oxidase family may indicate that this is the site of attachment of tetra- hydrofolate to the enzyme. c-Aminobutyrate ... generated during the catabolism of nicotine. The identiﬁcation of this enzyme reveals, for the ﬁrst time, the metabolic fate of the pyrrolidine ring of nicoti...

Ngày tải lên : 19/02/2014, 16:20

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Tài liệu Báo cáo khoa học: Local stability identiﬁcation and the role of a key aromatic amino acid residue in staphylococcal nuclease refolding pdf

... chain into N-terminal and C-terminal centers during refolding [21]. Since then, many scientists have been interested in demonstrating this concept by using N-terminal and C-terminal fragments of ... located in the C-terminal loop of SNase plays an important role in protein stability and conformational integrity. During protein unfolding ⁄ refolding, the addit...

Ngày tải lên : 20/02/2014, 01:20

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Báo cáo khoa học: Role of the C-terminal extension in a bacterial tyrosinase Michael Fairhead and Linda Thony-Meyer doc

... motif Arg40 Cys84 Tyr349 Tyr347 Lys370 Ala36 Ala36 Ala36 Val357 Phe518 B Laccase Tyrosinase β -sheet protein Fig. 1. Overview of the tyrosinase gene and surrounding genes in the genome of V. spinosum. (A) Showing the tyrosinase gene and those in its immediate vicinity in the ... meaning that the puriﬁcation of holo protein in this way was impracticable. C-ter...

Ngày tải lên : 06/03/2014, 11:20

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Báo cáo khoa học: Role of DptE and DptF in the lipidation reaction of daptomycin ppt

... between the hydroxyl group of Thr4 and the C-terminal Kyn13, resulting in a ten-membered ring [8]. More- over, several ORFs localized within the gene cluster are associated with the biosynthesis of ... of DptE, we expressed dptF using the pQTev vector in E. coli and puriﬁed the resulting ACP as an N-terminal His7 fusion protein (Fig. 3) with a yield of 9.5 mgÆL...

Ngày tải lên : 07/03/2014, 04:20

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Báo cáo khoa học: Role of different moieties from the lipooligosaccharide molecule in biological activities of the Moraxella catarrhalis outer membrane pot

... that the OSs from the wild-type strain consisted of terminal-, 2-, 4-, and 3,4,6-linked glucosyl residues, terminal- and 4-linked galactosyl residues, and a terminal-linked N-acetylglu- cosaminosyl ... mutant contained a truncated core OS and consisted of a linker (Glc-Kdo 2 )-lipid A structure. The phenotype of this mutant was examined to investigate the roles of di...

Ngày tải lên : 07/03/2014, 05:20

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