Báo cáo khoa học: Unusual ﬂuorescence of W168 in Plasmodium

Báo cáo khoa học: Unusual ﬂuorescence of W168 in Plasmodium falciparum triosephosphate isomerase, probed by single-tryptophan mutants docx

... 747 Monitoring of inhibitor binding to PfTIM and the mutants Quenching of Trp ﬂuorescence in all three proteins after binding of TIM inhibitor 2-phosphoglycolic acid was monitored at emission maxima of ... Biochem. 270) Ó FEBS 2003 Unusual ﬂuorescence of W168 in Plasmodium falciparum triosephosphate isomerase, probed by single-tryptophan mutants Pri...

Ngày tải lên : 31/03/2014, 07:20

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Báo cáo khoa học: Deciphering the key residues in Plasmodium falciparum b-ketoacyl acyl carrier protein reductase responsible for interactions with Plasmodium falciparum acyl carrier protein pptx

... report intensive studies on the direct interactions of Plasmo- dium FabG and Plasmodium ACP in solution, in the presence and absence of its cofactor, NADPH, by monitoring the change in intrinsic ﬂuorescence of ... number of cofactor-binding sites decreased in the presence of PfACP, the afﬁnity of PfFabG for NADPH increased, indicating a negative, heterotropic coope...

Ngày tải lên : 23/03/2014, 10:20

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Báo cáo khoa học: Unusual stability of human neuroglobin at low pH – molecular mechanisms and biological signiﬁcance docx

... Identiﬁca- tion of native and non-native structure in kinetic fold- ing intermediates of apomyoglobin. J Mol Biol 355, 139–156. 25 Jamin M & Baldwin RL (1996) Refolding and unfold- ing kinetics of the ... denaturation of Ngb and Mb. The denaturation of the proteins was monitored by recording the decrease in the ellipticity at 222 nm in the far-UV CD spectra of the pr...

Ngày tải lên : 07/03/2014, 02:20

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Tài liệu Báo cáo khoa học: Enhanced thermostability of methyl parathion hydrolase from Ochrobactrum sp. M231 by rational engineering of a glycine to proline mutation pdf

... quantiﬁcation of recombinant WT Ochr-MPH and mutants The N-terminus of each resulting recombinant protein was fused to a His6-tag that enabled puriﬁcation using a Ni-ni- trilotriacetic acid His-bindÔ resin ... September 2010) doi:10.1111/j.1742-4658.2010.07895.x Protein thermostability can be increased by some glycine to proline muta- tions in a target protein. However, not all glyc...

Ngày tải lên : 15/02/2014, 01:20

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Tài liệu Báo cáo khoa học: The occurrence of hemocyanin in Hexapoda docx

... proteins that evolved independently from hemocyanins [4]. Although hexamerins might be ubiquitous in insects [14,19–21], hemocyanin appears to be missing in eume- tabolous insects [22]. Here we investigate ... coordinated by three histidines in two distinct binding sites. Some hemocyanins assemble into large oligomers of up to 8 · 6 subunits [1]. The occurrence and properties of h...

Ngày tải lên : 18/02/2014, 08:20

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Tài liệu Báo cáo khoa học: The sequentiallity of nucleosomes in the 30 nm chromatin ﬁbre pptx

... the size of the original samples after digestion of the linkers, was sug- gested by Dorigo et al. [14] to indicate incomplete crosslinking. However, incomplete but random crosslinking should ... gradual decrease of the number of nucleosomes per chain in the main peak, with a corresponding increase in the Fig. 5. UV absorbance proﬁles of sucrose gradients of an oligonu- cle...

Ngày tải lên : 18/02/2014, 18:20

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Tài liệu Báo cáo khoa học: The role of antioxidants in the cytotoxicity of chemotherapeutic drugs pptx

... Quercetin did not inhibit ROS generation, and enhanced cytotoxicity of Topotecan in both cells. In contrast, NAC enhanced resistance against doxorubicine and vincristine in MRP1 overexpressing cells. ... their spleen. In addition, production of the cytokines interferon c, interleukin-6 and interleukin-2 is clearly different in wild type and in Ung-deﬁcient mice. Thus, Ung-prot...

Ngày tải lên : 19/02/2014, 07:20

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Tài liệu Báo cáo khoa học: Single phosphorylation of Tyr304 in the cytoplasmic tail of ephrin B2 confers high-afﬁnity and bifunctional binding to both the SH2 domain of Grb4 and the PDZ domain of the PDZ-RGS3 protein ppt

... 301–333) of ephrin B2 confers high-affinity binding to the SH2 domain of the Grb4 protein. Tyrosine phosphorylation at other candi- date sites resulted in only minor change of the binding of Tyr304-phosphorylated ... effect of tyrosine phosphorylations on the binding affinity of ephrinB2(301–333) to the SH2 domain of Grb4 and to the PDZ domain of PDZ-RGS3. In the combination ex...

Ngày tải lên : 19/02/2014, 12:20

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Tài liệu Báo cáo khoa học: "The Nature of Affixing in Written English" docx

... doubling rules in the inflection of English verbs C. The determination of word-breaking rules as used in end -of- the-line practices in type composition D. The determination of parts -of- speech ... leads to interest- ing results. In order to obtain a procedure for finding affixes, we will make use of one of the main results of reference 1. There we found that certain...

Ngày tải lên : 19/02/2014, 19:20

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Tài liệu Báo cáo khoa học: Gene transcription of fgl2 in endothelial cells is controlled by Ets-1 and Oct-1 and requires the presence of both Sp1 and Sp3 pdf

... proximal region of mouse chromosome 5 [14,15]. Fgl2 is a member of the fibrinogen family, which includes tenascin, cyto- toxin, and fibrinogen [16–18]. Fibrinogen-like protein family members all contain fibrinogen-related ... the pivotal role of fgl2 in a murine model for murine hepatitis virus strain 3 (MHV-3) induced fulminant hepatic failure [21,22]. Administration of neutralizing a...

Ngày tải lên : 20/02/2014, 11:20

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