Báo cáo khoa học: Comparative analysis of carbohydrate-binding properties of two tandem repeat-type Jacalin-related lectins, Castanea crenata agglutinin and Cycas revoluta leaf lectin docx
... specificity of Jacalin-related lectins
Comparative analysis of carbohydrate-binding properties
of two tandem repeat-type Jacalin-related lectins,
Castanea crenata agglutinin and Cycas revoluta leaf lectin
Sachiko ... analysis of these large mJRLs, i.e. C. crenata
agglutinin (CCA) and C. revoluta leave lectin (CRLL)
revealed that they form...
... deglycosylation of the fractions H
1
,M
1
and T
1
show
the disappearance of peaks between 3800 and 4600 Da for
hLf, and between 3000 and 3700 Da for the two recombin-
ant lactoferrins. In contrast, ... N-glycosylation of maize and tobacco lactoferrin (Eur. J. Biochem. 270) 3237
MALDI mass spectra of the glycopeptide fractions reveal
the heterogeneity of these fractions s...
... UÆmg
)1
protein and recovery levels of 20–
30%. In all cases, the analysis by SDS ⁄ PAGE and
native PAGE of purified preparations showed only
one protein band of 20–22 kDa (Figs 1 and 2; supple-
mentary ... Rao and Dr J.
Josse for critical review of the manuscript, and Profes-
sor W. Lo
¨
ffelhardt (University of Vienna, Austria) for
his assistance in N-terminal protein s...
... gene expression.
For comparative studies, in order to elucidate the
molecular basis of biological processes, the analysis of
dynamic changes of the subcellular distribution of gene
products is ... different kinds of information
exceeding the mere identification of proteins. These
comprise of studies on different part of the nucleus of
eukaryotic cells to demonstrate how p...
... and the CFU counted. The per-
centage of surviving CFU was calculated by the formula
N ⁄ N
control
· 100, where N and N
control
were the numbers of
CFU obtained after 3 h of incubation with and ... (2006)
Secretion of serine peptidase by a clinical strain of
Candida albicans: influence of growth conditions and
cleavage of human serum proteins and extracellular
matrix co...
... the present analysis of enzymatic properties
of ChiG, or by our analysis of the ChiG and ChiC
structures.
Despite the deletion of the B-loop, the two putative
catalytic residues Glu68 and Glu77 ... violet) and a small, presumably
amorphous, subfraction of a-chitin and b-chitin, but was not capable of
degrading crystalline chitin completely. The crystal structures...
... molecular mass of 103 kDa (composed from two
52 kDa monomers). The purified enzyme had optimum
activity at pH 3.0 and 40 °C and an isoelectric point of
9.8. The periplasmic localization of the enzyme ... decomposition of tetra-
thionate was proposed by Steudel et al.[16],Hazeuet al.
[17] and Meulenberg et al. [18] in experiments with intact
cells of A. ferrooxidans and A. a...
... 2011
Stability and fibril formation properties of human and
fish transthyretin, and of the Escherichia coli
transthyretin-related protein
Erik Lundberg
1
, Anders Olofsson
2
, Gunilla T. Westermark
3
and ... molecular basis of thermal
stability from the analysis of ion-pair networks in the
glutamate dehydrogenase family. Eur J Biochem 255,
336–346.
68 Sonnhammer EL & Dur...
... Søren Andersen and Professor
Peter Roepstorff (University of Southern Denmark,
Odense) for recording the presented MALDI-TOF ⁄
TOF spectrum, and Tove Olafsen and Anna M. Wu
(University of California ... Buus
2
and Inger Sandlie
1
1 Department of Molecular Biosciences and Centre for Immune Regulation, University of Oslo, Norway
2 Institute of Medical Microbiology and Immu...
... the two redox states of the
cofactor and that the distances from Ser23O to O1¢ and O2¢
are slightly different (Table 1) because of the different
positions and hybridizations of the C6 atom of ... hydroxylation of the cosubstrates [21,41].
However, the sequence of binding of cofactor and substrate
is still a matter of discussion and may be different comparing
wild-ty...