... Different stabilities of BIR family proteins in Xenopus inter-
phase egg extracts. (A) Stability of BIR family proteins in interphase
egg extracts. Purified MBP-tagged recombinants at 1 lgÆmL
)1
(upper) ... repeat, in which IAP
stands for inhibitor of apoptosis protein) domain is an
evolutionarily conserved zinc-binding motif. Many, but
not all, proteins conta...
... the binding of MP8 occurred through interactions
of its carboxylate substituents with amino acids of the
antibody, and that the protein provided a partial steric
hindrance of the distal face of ... of the Fe(II) spin state and no
replacement of any of the two axial ligands of the iron,
His18 or RNO, by an amino acid side-chain of the
antibody protein.
Binding site topology...
... 5. Increased plasma membrane stain-
ing of annexin II in G93A motor neurons.
Immunofluorescent staining of annexin II
and the neuronal marker neurofilament M
(NF-M) in spinal cord motor neurons in
90-day-old ... implicated in organizing
and compartmentalizing proteins involved in many
aspects of neurotransmitter signaling. These aspects
include transport of neurotransmitters...
... the
presence of heparin-binding motifs in various proteins
is well documented. This group of molecules includes
various laminin isoforms, fibronectin, coagulation factors,
growth factors, chemokines, ... report
c
a
Numbers indicate the position of the amino acids in the mature proteins.
b
Binding reported below 0.1
M
NaCl for the larger 15–42 peptide,
no binding for 18–31 [52].
c...
... (Beckton
Dickinson, Helsinki, Finland) of propidium iodide-stained
cells [70] or by the incorporation of [
3
H]thymidine in paral-
lel cell cultures.
UV cross-linking
UV cross-linking of proteins to ... to immuno-staining of pol d
followed by staining of PCNA (images A–E),
or immuno-staining of PCNA followed by
staining of pol e (images F–K). Immunolabel-
ling was visualized...
... chains of insulin [1] and
has an important structural role in the proper folding
and disulfide bonding in insulin [2]. After proinsulin
cleavage, it is released to the blood together with insu-
lin ... oligomers [12,13]. In the present
study, we found that, at 10 lm of insulin, the presence
Fig. 1. Oligomer formation of proinsulin
C-peptide is affected by metals and insulin.
Prosin...
... becom-
ing pupae in about a week after spinning (Fig. 1B).
Isovaleric acid seems to be the cause of prepupal
lethality because injection of isovaleric acid into
normal spinning larva induces ... the
sku mutant accumulates branched-chain amino acids in haemolymph, this
mutant may be useful in the investigation of unique branched-chain amino
acid catabolism in insects.
Abbreviatio...
... aimed to gain
further insight into the binding mode of different carbohydrates (cellobiose,
cellotetraose and cellohexaose) to the binding pocket of the family 11
CBM. The crystal structure of C. ... addition of CtCBM11.
In general, a progressive line broadening of all the
cellohexaose protons was observed during titration with
increasing amounts of protein, which can be under...
... chemokines stimu-
lated mainly by in ammatory cytokines, including IL-1b, TNF-a and
IFN-c. (B) Chemokines expressed in the joint recruit leukocytes into
the joints. In addition to functioning in ... examination of affected rheu-
matoid joints reveals extensive RANTES ⁄ CCL5
expression in the synovial lining and sublining layers
[22]. The expression of RANTES ⁄ CCL5 in cultured...
... that the N-terminal domain is involved in
protein–protein interactions with other hydrophilic domains
of neighboring integral membrane subunits, and interspecies
interactions are incompatible.
From ... the
bovine complex a large subdomain is made up of 20
integral membrane proteins contributing > 60 transmem-
brane segments. Some of these must be intimately involved
in proton...