Báo cáo khoa học: Identification of alternative promoter usage for the matrix Gla protein gene Evidence for differential expression during early development in Xenopus laevis doc
... ª 2005 FEBS
Identification of alternative promoter usage for the matrix
Gla protein gene
Evidence for differential expression during early development
in Xenopus laevis
Nate
´
rcia Conceic¸a
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o
1
*, ... mater-
nally inherited, and we provide evidence for the pres-
ence of alternative promoter usage in this gene during
early X. l...
... attached
to the lamina by integral membrane proteins of the
INM. The INM proteins form complexes, transiently
or stably, with lamins, chromatin proteins and a vari-
ety of regulatory proteins, including ... occurs between the DNA ⁄ protein com-
plexes of the heterochromatin protein BAF and emer-
in s amino-terminal LEM domain (aa residues 2–44)
[38], which similarly exist...
... mutations in 14 distinct resi-
dues, all facing the inside of the cell. Five of the residues lie
within the previously characterized N-terminal regulatory
domain and two mutations are located within the ... avoiding any side
chain conflicts, and bringing the N-loop in as centrally as
possible to the pore cavity, rotating the ends of the N-loop
toward transmembrane d...
... C-terminal region of RGS3 comprised the functional
domain for negative regulation of Gas protein. Since
then, alternatively spliced RGS protein isoforms were
detected for many RGS proteins. RGS8 alternatively
spliced ... an RGS8s protein with nine
amino acid deletion in the N-termini of RGS8 protein
[19,20]. The N-terminal deletion of RGS8 resulted in a
remarkable...
... that RGG3 of EWS binds mainly to the
G-quadruplex.
To test whether formation of the G-quadruplex is
necessary for RGG3 binding, we assayed the binding
of RGG3 with Htelo in the presence of K
+
or ... RGG domain of nucleolin,
a DNA-binding protein contributing to the transcrip-
tion of ribosomal RNA, bind to the G-quadruplex
forming ribosomal DNA [26]. Moreover, n...
... a-galactosyltransferase for addition
of the terminal galactose of the LOS [25], and Schwin-
gel et al. found the lgt6 gene involved in the initial
assembly of the LOS [26]. However, for lipid A
biosynthesis of the ... containing the
various agents were plated on the lawn in triplicate at
37 °C for 18 h. Sensitivity was assessed by measuring the
diameter...
... to
thermal stress are known to involve major adjustments
in the expression of ribosomal genes and genes coding
for proteins involved in RNA metabolism and protein
synthesis. In fact, protein ... but only in gills. The upregula-
tion of CTSL, combined with the downregulation of
its reversible binding inhibitor cystatin B, implies that
active protein degradation wa...
... different
domains of the protein, to determine the calcium-binding domain of
the protein. (A) Schematic drawing of the recombinant proteins. Six
GST-fused recombinant proteins containing the three distinctive
domains ... [4–7]. We therefore exam-
ined proteins contained in aggregates within the
otolith matrix and identified a protein contained in
the HMW protein...
... and the genes encoding these
enzymes.
The biosynthesis of triterpene saponins involves the
initial cyclization of 2,3-oxidosqualene, a common pre-
cursor of all the sterol and triterpene biosyntheses, ... [4–6]. In
order to improve the biological production method, a
detailed understanding of the biosynthesis of triterpene
saponins is required, including the enzymes...
... imply-
ing that the STAT2 DNA binding domain is essential
for the IFN-triggered expression of these genes: TLR3,
RBMS3. Closer examination of the IFN-inducible
expression of the TLR3 gene using ... Thus,
IFN-inducible expression of these genes is impaired in
the absence of the STAT2 DNA binding domain, sug-
gesting that their IFN-inducible transcription requi...