Báo cáo khoa học: Structure of the substrate complex of thymidine kinase from Ureaplasma urealyticum and investigations of possible drug targets for the enzyme pdf
Báo cáo khoa học: Structure of the substrate complex of thymidine kinase from Ureaplasma urealyticum and investigations of possible drug targets for the enzyme pdf
... Structure of the substrate complex of thymidine kinase from Ureaplasma urealyticum and investigations of possible drug targets for the enzyme Urszula Kosinska1*, ... posi-tion in the substrate complex from that in the complex with the feedbackinhibitor, having moved in closer to the 5¢-OH of the substrate to form ahydrogen bond. The phosphorylation of and inhibition ... anymember of the TK1 family, we have now determined the first structure of a member of the TK1 family in complex with the substrate dT. The possibilities for drug design have been investigated by enzyme...
- 8
- 392
- 0
Báo cáo khoa học: Val216 decides the substrate specificity of a-glucosidase in Saccharomyces cerevisiae doc
... locates on the long loop regionfollowed by Nb4, and the side chain of Val200 facestoward the inside of the c atalytic cleft [39]. Figure 4 shows the h ypothetical structure of the active site of S. ... notshown). These results indicate that the two recombinanta-glucosidases a re identical to their parent enzymes. Substrate specificities of chimeric enzymes The c omparison of the primary structures of ... from S. cerevisiae The production of maltase and isomaltase of S. cerevisiaewas induced by adding maltose and a-mg to the culturemedium, respectively [ 22]. In t he case of the cloning of the isomaltase...
- 7
- 452
- 0
Báo cáo khóa học: Interaction between the 2¢)5¢ oligoadenylate synthetase-like protein p59 OASL and the transcriptional repressor methyl CpG-binding protein 1 potx
... [12]. The products of these three genesare known, respectively, as the small (p42/p46), the medium(p69/p71) and the large (p100) forms of OAS [13], all of which are enzymatically active. The ... preylibrary from human leukocyte cDNA fused to the trans-activating domain of GAL4. The yeast strain L40 was used for screening the library, enabling selection of bait and preyplasmids by the TRP1 and ... critical for the covalent conjugation of Ub and UbLto cellular proteins [17]. Accordingly, the role of the p59OASL UbL is, as yet, unknown.An orthologue of p59 OASL exists in mice that, like the human...
- 9
- 419
- 0
Báo cáo khoa học: Kinetic studies on endo-b-galactosidase by a novel colorimetric assay and synthesis of N -acetyllactosamine-repeating oligosaccharide b-glycosides using its transglycosylation activity pptx
... 3. The catalytic efficiencies of 2 for bothenzymes were the highest among the synthetic substrates. The value of Vmax/Km of 2 for the E. freundii enzyme was1.7-times higher than that for ... °C. The enzyme solution was lyophilized and then used for synthesis of oligosaccharides without further purification. The crude enzyme preparation catalysed the transfer of a GlcNAcresidue from ... intensity of the enzyme was found tobe a plateau in the range of 20–40 mU for 15 min. The addition of 20 mU of coupled enzyme to the assay systemwas sufficient to obtain the maximal activity of endo-b-galactosidase...
- 11
- 365
- 0
Báo cáo khoa học: GHP, a new c-type green heme protein from Halochromatium salexigens and other proteobacteria potx
... [18] and an inacti-vated peptide deformylase [19]. The last of these is verylabile under oxygen stress, resulting in the oxidation of the catalytic Fe2+center of the deformylase into the inactive ... regions1–21 and 1–31, the former originating by incompletecleavage of a Glu-Val bond (Fig. 2). No evidence for cross-linking of the heme with other peptides of the protein has been found. The heme-containing ... 4B, which shows the MALDI MS ⁄ MS data for the precursor ion at 1975.0 Da. There is clearevidence for the presence of the further oxidationTable 1. Mass and sequence analysis of selected peptides...
- 11
- 517
- 0
Báo cáo khoa học: Schizosaccharomyces pombe positive cofactor 4 stimulates basal transcription from TATA-containing and TATA-less promoters through Mediator and transcription factor IIA ppt
... coding strand of the )35 to +6 region of Ad-MLP. (B) EMSAs used32P-labelled sin-gle-stranded DNA from the coding strand of the )35 to +6 region of Ad-MLP as the probe. The double- (dsDNA) and single-stranded(ssDNA) ... the stimulation of promoter escape as well as initiation in the presence of an activator. Therefore, we investigated the effect of PC4 on transcription at the level of preini-tiation complex formation, ... (asindicated at the bottom of the gel), for varying periods of time and then washed away. Transcription was then initiated by the addition of elongation mix. The lower band corresponds to an internalcontrol...
- 11
- 231
- 0
Báo cáo khoa học: Structure of a trypanosomatid mitochondrial cytochrome c with heme attached via only one thioether bond and implications for the substrate recognition requirements of heme lyase potx
... reason for the loss of one thioether bond from the mitochondrial cytochromes c of euglenozoans is alongstanding puzzle. It could be a means of altering the structure of these cytochromes c and ... details of the heme attach-ment. Notably, this similarity includes the stereochemistry of the covalentheme attachment to the protein. The structure has implications for the maturation of c-type ... chains that coordinate the heme iron, and the cysteine side chain that forms a thioether bondbetween heme and protein. Also shown is the methyl group of the alanine of the AXXCH heme-binding...
- 11
- 513
- 0
Tài liệu Báo cáo khoa học: Evidence that the assembly of the yeast cytochrome bc1 complex involves the formation of a large core structure in the inner mitochondrial membrane pdf
... addition of ISP and Qcr10p).On the other hand, in the transition from the 500 kDaband to the 670 kDa band, a structural rearrangement of the bc1 complex may occur due to the binding of ISP and ... 1909to the 500 kDa bc1sub -complex. Therefore, the pres-ence of both Qcr9p and Bcs1p is required for the inser-tion of ISP into the bc1sub -complex, but the presence of only one of these two ... describedpreviously [49,50]. Standard procedures were used for the preparation and ligation of DNA fragments, for the trans-formation of Escherichia coli and for the isolation of plas-mid DNA from bacterial...
- 15
- 639
- 0
Tài liệu Báo cáo khoa học: Structure of RNase Sa2 complexes with mononucleotides – new aspects of catalytic reaction and substrate recognition pptx
... molecule, the position of the guanine base and most of the phosphate groups of the first nucleotide (Gp) corresponded well with the mononucleotides in the crystal structures. The samewas true for the ... and the base of the third nucleotide are located in the vicinity of Thr61 and Arg67–Thr69. In the second cluster, the ribose and the base of the third nucleotide are close to Tyr32,Asn33, and ... corresponds to the position of the bound glucose. The presence of the third nucle-otide appears to influence the position of the base of the second nucleotide, which is turned by 90° and sandwiched...
- 13
- 523
- 0
Tài liệu Báo cáo khoa học: Structure of the putative 32 kDa myrosinase-binding protein from Arabidopsis (At3g16450.1) determined by SAIL-NMR docx
... steps per conformer wasperformed during the cyana structure calculations. In the final cycle of the cyana protocol, 100 conformers weregenerated and further refined using the amber 9 softwarepackage ... Research for Evolutional Science and Technology (CREST) of the Japan Science and Technology Agency (JST), by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science ... light of the possible biological function of this protein. Thisstudy demonstrates the power of the SAIL approachin determining the structure of a larger protein bysemi-automated means and with...
- 12
- 579
- 0
Từ khóa: Nghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngBáo cáo quy trình mua hàng CT CP Công Nghệ NPVchuyên đề điện xoay chiều theo dạngNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhPhát hiện xâm nhập dựa trên thuật toán k meansNghiên cứu tổng hợp các oxit hỗn hợp kích thƣớc nanomet ce 0 75 zr0 25o2 , ce 0 5 zr0 5o2 và khảo sát hoạt tính quang xúc tác của chúngNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Sở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXKiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)Tăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Nguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtChiến lược marketing tại ngân hàng Agribank chi nhánh Sài Gòn từ 2013-2015TÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲ